Bacteria-enzyme synergistically fermented feed containing stevia rebaudiana residues and application of feed

A technology of synergistic fermentation and stevia dregs, applied in applications, animal feed, animal feed, etc., can solve the problems of low added value of feed product sales, rough texture of stevia dregs, low acid-soluble protein content, etc., to improve feed Use value, reduce morbidity, and reduce the effect of somatic cell count

Pending Publication Date: 2020-09-22
博益德(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, stevia slag is a high-humidity material, and the added value of being sold directly or as a feed product after drying is low. The enthusiasm of enterprises is not high, and it is urgent to develop high value-added products.
Moreover, stevia rebaudiana dregs have rough texture, poor palatability, low acid-soluble protein content, and high fiber content, which affect their feeding value

Method used

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  • Bacteria-enzyme synergistically fermented feed containing stevia rebaudiana residues and application of feed
  • Bacteria-enzyme synergistically fermented feed containing stevia rebaudiana residues and application of feed
  • Bacteria-enzyme synergistically fermented feed containing stevia rebaudiana residues and application of feed

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 Stevia rebaudiana slag self-attached screening of lactic acid bacteria

[0023] The stevia slag (DM=60%) stacked naturally in the factory area of ​​Shandong Zhucheng Haotian Pharmaceutical Co., Ltd. was used as the strain source to carry out the enrichment culture of lactic acid bacteria. Take 1.0g stevia residue and add 9mL MRS culture medium (sucrose 20g / L, peptone 10g / L, beef extract 10g / L, yeast extract powder 5g / L, sodium acetate 5g / L, ammonium citrate 2g / L, K 2 HPO 4 2g / L, MgSO 4 ·7H 2 O0.58g / L, MnSO 4 4H 2 (0.25g / L, Tween 80 1.0mL / L, pH adjusted to 6.3, sterilized at 115°C for 20min) for enrichment culture: after static cultivation at 30°C for 24h, the inoculum size was 1% (volume fraction ratio) The transfer culture was continuously subcultured for 20 generations to obtain a lactic acid bacteria group with stable properties and strains.

[0024] The enriched culture solution was diluted and spread on the MRS agar medium for 36 hours at 30°C, ...

Embodiment 2

[0032] Inoculate the preserved Lactobacillus casei BFC190201 in the MRS liquid medium, and culture it statically at 25-37°C for 18-24h to obtain the primary seed liquid; ) into the new MRS medium again for secondary activation, after standing for 18-24h, the secondary seed liquid was obtained; the secondary seed liquid was separately inserted into the In the culture medium of the fermenter, the temperature is 25-37° C., and the static culture is carried out for 18-24 hours to obtain the fermentation broth of Lactobacillus casei BFC190201. Centrifuge the fermentation broth of Lactobacillus casei BFC190201 at 4°C at 8,000r / min for 10 minutes to obtain the sludge, add skim milk to the sludge at a ratio of 1:2~4 (m / v), shake and mix on a vortex shaker homogeneously, carry out vacuum freeze-drying, obtain Lactobacillus casei BFC190201 freeze-dried powder, the number of effective viable bacteria ≥ 1 × 10 12 CFU / g.

[0033] Lactobacillus plantarum P-8 was deposited in the General M...

Embodiment 3

[0037] Embodiment 3 contains the processing of stevia rebaudiana slag fermented feed

[0038] (1) Stevia rebaudiana slag aerobic fermentation pretreatment

[0039] Stevia rebaudiana dregs (DM=30%), molasses and urea are fully mixed according to the mass ratio of 93:6:1, inoculated with Aspergillus niger, put into a cement pool or fermentation tank, aerobic fermentation is fermented for 42h, and the temperature rises to Turn over and throw at 40°C to ensure that the fermentation temperature is lower than 40°C. As the fermentation time prolongs, the true protein content increases and the crude fiber content decreases. The results are shown in Table 2. After 24h, 36h and 48h of fermentation, the true protein content increases by 13.77%, 36.96% and 51.12%, respectively.

[0040] Table 2

[0041] True protein % Crude fiber% Before fermentation 15.61 18.32 Fermentation 24h 17.76 17.54 Fermentation 36h 21.38 15.55 Fermentation 48h 23.59 12....

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Abstract

The invention provides bacteria-enzyme synergistically fermented feed containing stevia rebaudiana residues and an application of the feed. The feed comprises the following raw materials in parts by mass: 38-43 parts of the stevia rebaudiana residues after pre-fermentation with aspergillus niger, 6-12 parts of corn, 4.5-12 parts of soybean meal, 12-20 parts of DDGS, 11-20 parts of rice bran meal,0-15 parts of bran, 0-8 parts of stone powder, 0-0.3 part of 30% betaine, 0-0.7 part of calcium hydrophosphate, 0-0.5 part of sodium chloride and 1-2 parts of glucose. A complex microbial agent accounting for 0.05-0.1% of the stevia rebaudiana residues is added to be mixed uniformly, a mixture is put into a breathing bag, the breathing bag is sealed, and fermentation is performed at 25-30 DEG C for 5-7 days. The feed can improve the production performance of laying hens and improve the air quality in a laying hen house; the slaughtering rate and the lean meat percentage of pigs can be increased, and the backfat thickness and the incidence rate are reduced; and the dry matter intake and the milk yield of dairy cows can be effectively improved, the number of somatic cells in milk is reduced,and the incidence rate of dairy cow mastitis is reduced.

Description

technical field [0001] The invention belongs to the technical field of fermented feed, and in particular relates to a bacteria-enzyme synergistic fermented feed containing stevia rebaudiana dregs and an application thereof. Background technique [0002] Stevia sugar products will produce a large amount of stevia slag during the sugar making process. The stevia slag produced in my country is more than 200,000 tons every year. Stevia slag belongs to industrial waste, and the treatment cost is very high. At present, the main treatment methods of stevia waste residue are incineration and composting. Incineration pollutes the environment, and composting prevents the nutrients and active substances in it from being used reasonably and efficiently, resulting in waste of resources. How to effectively deal with stevia slag is not only related to environmental issues, but also affects the extension of the stevia deep processing industry chain and the benefits of enterprises. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/30A23K10/33A23K10/37A23K20/105A23K20/163A23K20/20A23K20/26A23K50/15A23K50/30A23K50/75
CPCA23K10/12A23K10/30A23K10/33A23K10/37A23K20/105A23K20/163A23K20/20A23K20/26A23K50/15A23K50/30A23K50/75A23V2400/125A23V2400/169Y02P60/87
Inventor 刘晶晶田子罡陈达傅杰任婉丽于继英
Owner 博益德(北京)生物科技有限公司
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