Bacillus subtilis and application thereof in fermentation treatment of flaxseed cake detoxification

A technology of Bacillus subtilis and linseed cake, which is applied in the field of microorganisms, can solve the problems of affecting the nutrient content of linseed, reducing the nutritional value, and the equipment is not yet perfect, so as to inhibit the reproduction of animal gastrointestinal pathogens, increase the content of polypeptides, and promote the intestinal tract. The effect of micro-ecological balance

Inactive Publication Date: 2020-09-22
FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although various detoxification methods can significantly reduce the content of cyanogenic glycosides in flaxseed, these methods all have defects in varying degrees, such as the detoxification rate is not high enough, or the equipment is not perfect, or the cost is high, or cause secondary pollution. Pollution, it is difficult to apply in actual production, most of the processing technology will affect the nutritional content of flaxseed and reduce the nutritional value

Method used

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  • Bacillus subtilis and application thereof in fermentation treatment of flaxseed cake detoxification
  • Bacillus subtilis and application thereof in fermentation treatment of flaxseed cake detoxification
  • Bacillus subtilis and application thereof in fermentation treatment of flaxseed cake detoxification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, the separation and purification of Bacillus subtilis and identification

[0059] 1.1 Isolation and purification of Bacillus subtilis

[0060] 1.1.1 Test materials

[0061] Flaxseed cake was purchased from Hohhot Mengguxiang Biotechnology Co., Ltd.

[0062] Sheep rumen juice was collected from the pilot base of the Chinese Academy of Agricultural Sciences and stored at 4°C.

[0063] The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores.

[0064] 1.1.2 Medium

[0065] Bacterial screening solid medium (escin-LB solid medium): yeast extract 0.5g, agar powder 1.5g, peptone 1g, sodium chloride 1g, aescin 0.05g, ferric citrate 0.01g, distilled water 100mL, Sterilize at 121°C for 20 minutes.

[0066] Bacterial basal liquid medium (LB liquid medium): 0.5g of yeast extract, 1g of peptone, 1g of sodium chloride, 100mL of distilled water, sterilized at 121°C for 20min.

[0067] ...

Embodiment 2

[0096] Example 2. Research on the function of degrading cyanogenic glycosides by Bacillus subtilis BS fermented flaxseed cake

[0097] 2.1 Determination of BS growth curve of Bacillus subtilis

[0098] Streak culture on the isolated Bacillus subtilis BS plate, pick a single colony on the plate and put it in 10mL LB liquid medium and place it on a shaking table at 37°C and 200r / min for 24h to make a seed solution, and add 1% The inoculum size was inoculated into 250mL liquid LB medium and placed on a shaking table at 37°C and 200r / min for 36h. Samples were taken every 2h, and the optical density (OD) value was measured at 600nm with a UV-visible spectrophotometer. 600 The value represents the growth of the bacteria, and the OD is plotted 600 Graph versus time.

[0099] image 3 is the growth curve of Bacillus subtilis BS. Such as image 3 As shown, Bacillus subtilis BS went through a short slow growth period, and was in the logarithmic growth phase from 2h to 24h, reached ...

Embodiment 3

[0123] Example 3, Process Optimization of Bacillus subtilis BS Fermentation Treatment of Flaxseed Cake Degradation of Cyanogenic Glycosides

[0124] 3.1 Measuring indicators and methods

[0125] Refer to the colorimetric method in GB / T13084-2006 to determine the content of cyanogenic glycosides, use Dumas automatic azotometer to measure crude protein content, use filter bag method to determine crude fiber content, and use Soxhlet extraction method to determine crude fat Content, refer to the determination method of GB / T22492-2008 soybean peptide powder to determine the peptide content.

[0126] The results of all the above determination indicators are based on absolute dry mass.

[0127] 3.2 Data statistics and analysis

[0128] The experimental data was established using Excel as a database, and analyzed by one-way analysis of variance in SPSS 19.0 software. Except for the orthogonal test, the results of other tests are expressed in the form of mean ± standard error, and P...

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Abstract

The invention discloses bacillus subtilis BS. The preservation registration number of the bacillus subtilis BS is CGMCC No.18229, and the bacillus subtilis BS is preserved in China General Microbiological Culture Collection Center with the address of No.3, No.1 Yard, Beichen West Road, Chaoyang District of Beijing, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China, the postcode is 100101, and the preservation time is July 15, 2019. The invention also discloses a culture of the bacillus subtilis BS and a bacterial suspension of the bacillus subtilis BS. The invention further discloses a product, and the active ingredient of the product is bacillus subtilis BS, a culture of the bacillus subtilis BS or the bacterial suspension of the bacillus subtilis BS. The invention also discloses application of the bacillus subtilis BS, the culture of the bacillus subtilis BS or the bacterial suspension of the bacillus subtilis BS, and the application is as follows: a) degrading cyanogenic glycoside, or b) degrading macromolecular protein in the flaxseed cake, or c) producing polypeptide.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a strain of Bacillus subtilis and the application of the Bacillus subtilis in the aspect of fermentation treatment for detoxification of linseed cake. Background technique [0002] Flax (Linum ustitatissimum L.), also known as flax, belongs to the family Linaceae and the genus Flax. Flax is one of the oldest fiber crops in the world. There are many varieties, but they can be roughly divided into three categories: flax for oil, flax for fiber and flax for oil and fiber. One of the oil crops. According to statistics, the global annual output of flaxseed in 2018 was about 2.6 million tons, ranking seventh in the world's total oil production. Flax for oil is mainly produced in Canada, China, Argentina, and the United States. [0003] As one of the important oil crops in my country, flax for oil is an important economic crop in my country, and it is also the main oil c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L5/20A23K10/18A23K10/12C12R1/125
CPCA23K10/12A23K10/18A23L5/28C12N1/205C12R2001/125
Inventor 蔡辉益郭宝珠刘国华
Owner FEED RESEARCH INSTITUTE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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