Bacillus subtilis and application thereof in fermentation treatment of flaxseed cake detoxification
A technology of Bacillus subtilis and linseed cake, which is applied in the field of microorganisms, can solve the problems of affecting the nutrient content of linseed, reducing the nutritional value, and the equipment is not yet perfect, so as to inhibit the reproduction of animal gastrointestinal pathogens, increase the content of polypeptides, and promote the intestinal tract. The effect of micro-ecological balance
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Embodiment 1
[0058] Embodiment 1, the separation and purification of Bacillus subtilis and identification
[0059] 1.1 Isolation and purification of Bacillus subtilis
[0060] 1.1.1 Test materials
[0061] Flaxseed cake was purchased from Hohhot Mengguxiang Biotechnology Co., Ltd.
[0062] Sheep rumen juice was collected from the pilot base of the Chinese Academy of Agricultural Sciences and stored at 4°C.
[0063] The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores.
[0064] 1.1.2 Medium
[0065] Bacterial screening solid medium (escin-LB solid medium): yeast extract 0.5g, agar powder 1.5g, peptone 1g, sodium chloride 1g, aescin 0.05g, ferric citrate 0.01g, distilled water 100mL, Sterilize at 121°C for 20 minutes.
[0066] Bacterial basal liquid medium (LB liquid medium): 0.5g of yeast extract, 1g of peptone, 1g of sodium chloride, 100mL of distilled water, sterilized at 121°C for 20min.
[0067] ...
Embodiment 2
[0096] Example 2. Research on the function of degrading cyanogenic glycosides by Bacillus subtilis BS fermented flaxseed cake
[0097] 2.1 Determination of BS growth curve of Bacillus subtilis
[0098] Streak culture on the isolated Bacillus subtilis BS plate, pick a single colony on the plate and put it in 10mL LB liquid medium and place it on a shaking table at 37°C and 200r / min for 24h to make a seed solution, and add 1% The inoculum size was inoculated into 250mL liquid LB medium and placed on a shaking table at 37°C and 200r / min for 36h. Samples were taken every 2h, and the optical density (OD) value was measured at 600nm with a UV-visible spectrophotometer. 600 The value represents the growth of the bacteria, and the OD is plotted 600 Graph versus time.
[0099] image 3 is the growth curve of Bacillus subtilis BS. Such as image 3 As shown, Bacillus subtilis BS went through a short slow growth period, and was in the logarithmic growth phase from 2h to 24h, reached ...
Embodiment 3
[0123] Example 3, Process Optimization of Bacillus subtilis BS Fermentation Treatment of Flaxseed Cake Degradation of Cyanogenic Glycosides
[0124] 3.1 Measuring indicators and methods
[0125] Refer to the colorimetric method in GB / T13084-2006 to determine the content of cyanogenic glycosides, use Dumas automatic azotometer to measure crude protein content, use filter bag method to determine crude fiber content, and use Soxhlet extraction method to determine crude fat Content, refer to the determination method of GB / T22492-2008 soybean peptide powder to determine the peptide content.
[0126] The results of all the above determination indicators are based on absolute dry mass.
[0127] 3.2 Data statistics and analysis
[0128] The experimental data was established using Excel as a database, and analyzed by one-way analysis of variance in SPSS 19.0 software. Except for the orthogonal test, the results of other tests are expressed in the form of mean ± standard error, and P...
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