Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation and application of recombinant porcine epidermal growth factor

A technology of epidermal growth factor and gene, applied in the field of genetic engineering, to avoid drug resistance and environmental pollution, improve economic benefits, and promote intestinal epithelial cell proliferation

Inactive Publication Date: 2020-09-25
WUHU YINGTE FEIER BIOLOGICAL PROD IND RES INST CO LTD +1
View PDF4 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Technical problem to be solved by the present invention: solve many problems existing in existing piglet feed additives

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and application of recombinant porcine epidermal growth factor
  • Preparation and application of recombinant porcine epidermal growth factor
  • Preparation and application of recombinant porcine epidermal growth factor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1: prepare the preparation of novel active protein as follows:

[0041] 1.1 Construction of recombinant porcine EGF expression plasmid

[0042] The relevant gene and nucleotide sequence of recombinant porcine epidermal growth factor (SEQ ID NO.1) were obtained through GenBank query, and E. 28a-rpEGF and pET-28a-SUMO-rpEGF expression plasmids.

[0043] 1.2 Induced expression of recombinant porcine EGF engineering bacteria

[0044] The constructed expression plasmids pET28a-rpEGF, pET32a-rpEGF and pET28a-SUMO-rpEGF were respectively transferred into competent cells prepared by BL21 / DE3.0. : 100 (for example, 2ml strains are inoculated into 100ml medium) inoculated in LB liquid medium containing resistance, cultured at 37°C with shaking at 220r / min until OD 600 When it is 0.5 to 0.6, add IPTG (to make the final concentration 0.5mmol / L) to induce expression for about 5 hours, take 1ml of the bacterial liquid induced to express, centrifuge at 12000r / min at 4°C ...

Embodiment 2

[0049] Embodiment 2: the application of novel active protein in feed additive:

[0050] EGF involves the regulation of a large number of ion channels and transport proteins, and promotes the absorption of nutrients in the intestine, such as regulating the absorption of phosphorus in the small intestine, maintaining normal cell functions, and promoting the absorption of glutamine by the epithelium. The special structure of EGF makes it highly resistant to heat and acid, and can resist digestion by trypsin and pepsin, which determines that it can act on the gastrointestinal tract for a long time. After oral administration of EGF to young piglets, it still retains its biological activity in the intestinal tract, promoting the growth and maturation of the gastrointestinal tract after birth.

[0051] Relevant literature shows that pEGF is beneficial to the early embryonic development of pigs, increasing the litter size of sows, improving the survival rate of piglets and promoting t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses preparation and application of a recombinant porcine epidermal growth factor, and relates to the field of gene engineering. The recombinant porcine epidermal growth factor is prepared through expression of a prokaryotic expression system. The EGF prepared by genetic engineering is expressed by thallus breaking supernatant, purification is easy, the production period is short, mass production can be achieved through downstream high-density fermentation, the cost is saved, the purity and activity of protein are high, and no toxic and side effects exist. The recombinant porcine EGF is directly fed, so that the operation process can be simplified, extra damage caused by injection fault or stimulation is reduced, and the economic benefit is improved. The recombinant porcine EGF protein is used for replacing traditional antibiotics to relieve weaning stress, so that the diarrhea rate can be reduced, proliferation of intestinal epithelial cells is promoted, the damagedintestinal tracts are repaired, and drug resistance and environmental pollution caused by long-term use of the antibiotics are avoided.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to the preparation and application of a recombinant porcine epidermal growth factor. Background technique [0002] During the development of China's feed additive industry, plasma protein powder is also an excellent feed material with high protein, high energy and high minerals. However, due to the uneven technological level and product quality of various manufacturers of blood products for feeding, the complete inactivation of pathogenic microorganisms cannot be guaranteed, so the risk of infection is extremely high. [0003] Drug feed additives will all be withdrawn in 2020. Obviously, antibiotic reduction / replacement in animal husbandry is the general trend. With the development of feed additives in the direction of green, high efficiency, safety and multi-function, the development of new safe additives with specific biological activities and functions by modern biotechnology ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/37A23K20/10A23K20/105A23K20/147A23K20/153A23K50/30C07K14/485C12N15/70C12N1/21C12R1/19
CPCA23K50/30A23K20/153A23K20/147A23K20/10A23K20/105A23K10/37C07K14/485C12N15/70Y02P60/87
Inventor 单雪芹刘家炉刘洁许高涛李媛媛袁蕾缪圣孔国庆张超夏兵兵
Owner WUHU YINGTE FEIER BIOLOGICAL PROD IND RES INST CO LTD