Preparation method of urea determination reagent ball, reagent ball and detection chip

A urea and reagent technology, applied in the field of urea detection, can solve the problems of narrow effective measurement range and poor thermal stability of urea reagent, and achieve the effect of wide effective detection range, not easy to deteriorate, and good thermal stability

Pending Publication Date: 2020-09-25
GENRUI BIOTECH INC
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Problems solved by technology

[0005] In order to overcome the problems of poor thermal stability and narrow effective measurement range of urea reagents, the embodiment of the present invention provides ...

Method used

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  • Preparation method of urea determination reagent ball, reagent ball and detection chip
  • Preparation method of urea determination reagent ball, reagent ball and detection chip
  • Preparation method of urea determination reagent ball, reagent ball and detection chip

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preparation example Construction

[0037] The embodiment of the present invention provides a method for preparing a urea assay reagent ball, which can prepare a spherical urea assay reagent. Please refer to figure 1 , the method includes the following steps:

[0038] S11. Mix buffer, disodium edetate, α-ketoglutarate, glutamate dehydrogenase, urease, reduced nicotinamide adenine dinucleotide and excipients with water to form a mixture liquid, and adjust the pH value of the mixed solution;

[0039] Specifically, measure a certain volume of buffer solution and add it to a container containing 800ml of water; after the buffer solution is completely dissolved in water, add a certain amount of disodium edetate and α-ketoglutaric acid and reduced nicotinamide adenine dinucleotide; after adjusting the pH value of the solution in the container to 7.0, add glutamate dehydrogenase and urease to the container, and then add excipients; finally add water to the container , to adjust the volume of the solution in the conta...

Embodiment 1

[0053] In this embodiment, the buffer solution is composed of sodium dihydrogen phosphate dihydrate and disodium hydrogen phosphate dodecahydrate, and the excipients are composed of trehalose and sodium cholate. The preparation method of the urea determination reagent ball is as follows: a certain amount of sodium dihydrogen phosphate dihydrate buffer, disodium hydrogen phosphate dodecahydrate buffer, disodium edetate, α-ketoglutaric acid, glutamic acid Dehydrogenase, urease, reduced nicotinamide adenine dinucleotide, trehalose and sodium cholate are mixed with water to form a mixed solution, and the pH value of the mixed solution is adjusted to 7.0; An ice ball with a volume of about 4.0 ul was formed in liquid nitrogen; the ice ball was freeze-dried to obtain the urea assay reagent ball. And the urea detection chip was prepared by using the urea determination reagent ball.

[0054] Specifically, the mixed solution in this embodiment includes the following components: 9.12 g...

Embodiment 2

[0056] In this embodiment, the buffer is tris, and the excipients include dextran 40,000 and mannitol. The preparation method of the urea determination reagent ball is as follows: a certain amount of Tris buffer solution, disodium edetate, α-ketoglutaric acid, glutamate dehydrogenase, urease, reduced nicotinamide Adenine dinucleotide, dextran 40,000 and mannitol are mixed with water to form a mixed solution, and the pH value of the mixed solution is adjusted to 7.0; the droplets of the mixed solution are dropped in liquid nitrogen to form ice balls with a volume of about 3.0 ul of ice balls; the ice balls were freeze-dried to obtain the urea assay reagent balls. And the urea detection chip was prepared by using the urea determination reagent ball.

[0057] Specifically, the mixed solution in this embodiment includes the following components: 24.2 g / L of trishydroxymethylaminomethane, 3.87 g / L of disodium edetate, 4.72 g / L of α-ketoglutarate, gluten Amino acid dehydrogenase 5...

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Abstract

The invention relates to the technical field of urea detection, in particular to a preparation method of a urea determination reagent ball, the reagent ball and a detection chip. The embodiment of theinvention provides a preparation method of a urea determination reagent ball. The method comprises the following steps of mixing a certain dosage of buffer solution, disodium ethylene diamine tetraacetate, alpha-ketoglutarate, glutamate dehydrogenase, urease, reduced nicotinamide adenine dinucleotide and excipient with water to form a mixed solution, dropwise adding the mixed solution into liquidnitrogen to enable liquid drops of the mixed solution to form ice balls, and freeze-drying the ice balls to obtain spherical urea determination reagent balls. The urea determination reagent ball prepared by the method has good thermal stability, can be stored for 8 days at a high temperature of 37 DEG C, is not easy to deteriorate in the storage process, has a wide effective test range on urea inblood, and can test the highest concentration of blood urea as high as 35mmol/L.

Description

technical field [0001] The invention relates to the technical field of urea detection, in particular to a method for preparing a urea determination reagent ball, a reagent ball and a detection chip. Background technique [0002] Amino acids in the human body are decomposed into α-keto acids and NH by deamination 3 , NH 3 Enter the urea cycle and CO in hepatocytes 2 This produces urea. The urea produced in the liver is mainly excreted by the kidneys through the blood circulation. [0003] Under normal circumstances, the non-protein nitrogen in the blood is mainly filtered out by the glomeruli and excreted with the urine. Non-protein nitrogen in blood refers to nitrogen-containing compounds other than plasma protein, such as urea, uric acid, creatine, creatinine, and amino acids, among which urea nitrogen accounts for about 50%. When the renal parenchyma is damaged, the concentration of non-protein nitrogen in the blood increases due to the decrease of the filtration rate...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N1/38B01L3/00
CPCG01N21/33G01N1/38B01L3/502707
Inventor 汪晨宇黎广来高玉丹
Owner GENRUI BIOTECH INC
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