Bacteroidetes and their applications
A technology of Bacteroides and Bacteroides saccharides, applied in the field of microbiology, can solve the problems of no research discovery and small research scale, and achieve the effect of weight control and blood lipid improvement
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Embodiment 1
[0027] Isolation and Purification of Bacteroide
[0028] Using aseptic operation, scrape 50 mg of fresh feces from healthy volunteers with a glass slide, place it in a glass test tube containing 9 mL of sterile saline, shake it well, and then suck 0.5 mL of the mixture into a sterile 4.5 mL test tube. In a test tube of normal saline, this dilution is 10 -1 , repeat the above process for 10-fold dilution to 10 -6 Dilution, choose 10 -4 , 10 -5 , 10 -6 Three dilutions, 0.1 mL of bacterial droplets were drawn on the MRS medium plate, and anaerobic culture method (5% CO) was used after the plate was coated. 2 ) Place the coated petri dish in a 37°C incubator for 48h.
[0029] Using the four-division streaking method, use the inoculation loop to pick colonies with different shapes on the MRS agar medium for streak separation and culture. After 48 hours of culture, select the colonies with good separation effect in the four-division zone and inoculate the MRS with the inoculati...
Embodiment 2
[0032] Effects of Bacteroides on ob / ob blood glucose, blood lipids and body weight in obese and diabetic model mice
[0033] Material:
[0034] The tested strains are: Bacteroides xylanisolvens (abbreviation: BX), Bacteroides dorei (abbreviation: BD), Bacteroides ovatus (abbreviation: BO).
[0035] The amount of gavage bacteria was determined as 0.15mL / 10g body weight, and the bacterial concentration was 1×10 7 cfu / mL, the concentration after concentration is 1×10 9 cfu / mL, the frequency is once daily.
[0036] The bacterial liquid should be cultured in advance, activated every week to ensure freshness, and the concentration should be determined separately.
[0037]Ob / ob mice were purchased from Institute of Zoology, Chinese Academy of Medical Sciences and Peking Union Medical College, temperature 20-24 degrees Celsius, constant humidity 50-60%, light for 12 hours (8:00-20:00), sound insulation, free food and water , the experiment was carried out after one week of acclima...
Embodiment 3
[0053] Effects of Bacteroides xylanisolvens and its probiotic composition on ob / ob blood glucose, body weight, blood lipids and liver function in hyperglycemic mice
[0054] Material:
[0055] The tested strains were Bacteroides xylanisolvens (BX for short), Lactobacillus gasseri (Lactobacillus gasseri), Lactobacillus rhamnosu GM-020, Clostridium butyricum (Clostridium butyricum), double The amount of Bifidobacterium lactis BB12 by gavage was determined as 0.15mL / 10g body weight, and the frequency was once a day.
[0056] The bacterial liquid should be cultured in advance, activated every week to ensure freshness, and the concentration should be determined separately.
[0057] Ob / ob mice were purchased from Institute of Zoology, Chinese Academy of Medical Sciences and Peking Union Medical College, temperature 20-24 degrees Celsius, constant humidity 50-60%, light for 12 hours (8:00-20:00), sound insulation, free food and water , the experiment was carried out after one week ...
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