Method for preparing magnetic biochar material through iron-rich microorganism conversion
A bio-carbon material and microbial conversion technology, applied in chemical instruments and methods, alkali metal oxides/hydroxides, inorganic chemistry, etc., can solve problems such as increased costs, and achieve easy cultivation, simple production process, easy recycling and The effect of reuse
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Embodiment 1
[0022] Use distilled water to prepare liquid medium, the main component is peptone 0.5 g L -1 , glucose 1 g L -1 , yeast extract powder 0.5 g L -1 , anhydrous calcium chloride 0.222 g L -1 , anhydrous magnesium sulfate 0.396 g L -1 , aliquot 100 mL into Erlenmeyer flasks, and maintain at 115 °C for 30 min for sterilization. Prepare ferric ammonium citrate mother liquor 100 g L -1 , sterilized under the same conditions. Put Phanerochaete chrysosporium spores into the liquid medium in a clean operating bench, and then add 0.2 mL ferric ammonium citrate mother solution. The culture medium was placed in a constant temperature shaker, cultured at 180 rpm and 30 °C for 72 h, collected and freeze-dried to remove water to obtain mycelial balls. Add 1 g of dried mycelium balls into the crucible, set the temperature at 400 °C, and carbonize at high temperature for 2 h under a protective atmosphere. After cooling to room temperature, take it out and grind it into powder to obtain ...
Embodiment 2
[0024] Use distilled water to prepare liquid medium, the main component is peptone 0.5 g L -1 , glucose 1 g L -1 , yeast extract powder 0.5 g L -1 , anhydrous calcium chloride 0.222 g L -1 , anhydrous magnesium sulfate 0.396 g L -1 , aliquot 100 mL into Erlenmeyer flasks, and maintain at 115 °C for 30 min for sterilization. Prepare ferric ammonium citrate mother liquor 100 g L -1 , sterilized under the same conditions. Put Phanerochaete chrysosporium spores into the liquid medium in a clean operating bench, and then add 3 mL ferric ammonium citrate mother solution. The culture medium was placed in a constant temperature shaker, cultured at 180 rpm and 30 °C for 72 h, collected and freeze-dried to remove water to obtain mycelial balls. Add 1 g of dried mycelium balls into the crucible, set the temperature at 400 °C, and carbonize at high temperature for 2 h under a protective atmosphere. After cooling to room temperature, take it out and grind it into powder to obtain th...
Embodiment 3
[0026] Use distilled water to prepare liquid medium, the main component is peptone 0.5 g L -1 , glucose 1 g L -1 , yeast extract powder 0.5 g L -1 , anhydrous calcium chloride 0.222 g L -1 , anhydrous magnesium sulfate 0.396 g L -1 , aliquot 100 mL into Erlenmeyer flasks, and maintain at 115 °C for 30 min for sterilization. Prepare ferric ammonium citrate mother liquor 100 g L -1 , sterilized under the same conditions. Put Phanerochaete chrysosporium spores into the liquid medium in a clean operating bench, and then add 1 mL ferric ammonium citrate mother solution. The culture medium was placed in a constant temperature shaker, cultured at 180 rpm and 30 °C for 72 h, collected and freeze-dried to remove water to obtain mycelial balls. Add 1 g of dried mycelium balls into the crucible, set the temperature at 400 °C, and carbonize at high temperature for 2 h under a protective atmosphere. After cooling to room temperature, take it out and grind it into powder to obtain th...
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