Laminar flow liquid for gynecological secretion analysis instrument and preparation method of laminar flow liquid
A technology for analyzing instruments and secretions, which is applied in the field of laminar flow liquid for gynecological secretion analysis instruments and its preparation, can solve the problems of lack of automation, standardization, high clinical experience requirements, low sensitivity, etc., and achieve good real-time stability, Avoid damage to production personnel and the environment, and facilitate the preparation
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[0059] A preparation method of laminar flow liquid for a gynecological secretion analysis instrument of the present invention comprises the following steps:
[0060] Step 1. Stir and fully dissolve buffer, surfactant, chelating agent and stabilizer with purified water;
[0061] Step 2. Pour the organic solvent into the solution obtained in Step 1, continue to stir and fully dissolve;
[0062] Step 3, add antibacterial agent to the solution obtained in step 2, add purified water to constant volume;
[0063] The pH value of the solution obtained in step 4 and step 3 is 5.5-8.0, the conductivity is 10-12mS / cm, the osmotic pressure is 240-300mmol / L, and the refractive index is 1.333-1.345;
[0064] Step 5: Filter the prepared solution to obtain a laminar flow solution, which is stored at 2° C. to 30° C.
[0065] It should be noted that in step 2, the organic solvent needs to be fully dissolved.
[0066] The invention provides a laminar flow liquid suitable for the flow cytometr...
Embodiment 1
[0071] Embodiment 1 Preparation of laminar flow liquid for gynecological secretion analysis instrument
[0072] 1) Stir and fully dissolve 0.05% acetate buffer, 0.5% Bolesham 188, 0.02% EDTA-2Na, 0.15% stabilizer (sodium chloride and potassium chloride) in purified water;
[0073] 2) Pour 0.05% glycerol into the solution obtained in step 1), continue to stir and fully dissolve;
[0074] 3) Continue to add 0.2% of 2-methyl-4-isothiazolin-3-one to step 1), and add purified water to constant volume;
[0075] 4) The pH value of the solution obtained in step 3) is 5.5-8.0, the conductivity is 10-12 mS / cm, the osmotic pressure is 240-300, and the refractive index is 1.333-1.345;
[0076] 5) Filter the prepared solution with a filter to obtain a laminar flow liquid, which is stored at 2°C to 30°C.
Embodiment 2
[0077] Example 2 Performance evaluation of laminar flow liquid for gynecological secretion analysis instrument
[0078] 1) pH value: take an appropriate amount of laminar flow liquid, measure its pH value at 25±1°C with an acidity meter with an accuracy of 0.01, and measure it three times in a row.
[0079] 2) Conductivity: use an osmometer to measure the osmotic concentration value of the laminar fluid, and measure three times continuously.
[0080] 3) Osmotic concentration: measure the refractive index / specific gravity of the laminar fluid with a hydrometer, and measure three times continuously.
[0081] 4) Refractive index: measure the refractive index / specific gravity of the laminar fluid with a hydrometer, and measure three times continuously.
[0082] 5) Blank value: Empty the original reagents inside the matching automatic gynecological secretion analysis system, introduce the laminar flow fluid to be tested, fill it twice, and conduct blank measurements continuously f...
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