Bacillus culture medium

A technology of bacillus and culture medium, applied in the field of agricultural microorganisms, can solve problems such as troubles and increased costs

Pending Publication Date: 2020-10-09
GUANGXI VETERINARY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are very cumbersome to operate in practice, and also increase the cost

Method used

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  • Bacillus culture medium
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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1, be used for the screening of the basal medium of cultivating Bacillus licheniformis / Bacillus subtilis

[0045] The preparation of described basal medium

[0046] 1. Preparation of nutrient broth medium Weigh 19.0 g of nutrient broth medium powder into 1 L of distilled water, heat and boil until completely dissolved, then dispense into test tubes, 10 mL / tube, autoclave at 121 °C for 15 min After cooling to room temperature, the basal culture medium was obtained.

[0047] 2. Preparation of TSB Broth Medium Weigh 30.0 g of TSB medium powder into 1 L of distilled water, heat and boil until completely dissolved, then dispense into test tubes, 10 mL / tube, autoclave at 121 °C for 15 min, and cool After reaching room temperature, the basal culture medium was obtained.

[0048] 3. Preparation of LB broth medium Weigh 25.0 g of LB medium powder into 1 L of distilled water, heat and boil until completely dissolved, then dispense into test tubes, 10 mL / tube, autocla...

Embodiment 2

[0055] Embodiment 2, the selection of optimal carbon source

[0056] The medium to be tested consists of TSB broth basal medium containing 2 parts by weight of carbon sources (glucose, sucrose, lactose).

[0057] 1. Inoculate 100 μL of the seed solution into a test tube containing 10 mL of the medium to be tested (the inoculum volume is 1% (v / v)), then place it on a constant temperature shaker, and incubate with shaking at 37 °C and 200 rpm for 18 h. Obtain a fermented broth.

[0058] 2. After completing step 1, measure the OD of the fermentation broth by ultraviolet spectrophotometry 600nm value.

[0059] The test results are shown in Table 2. The results showed that when the carbon source in the medium to be tested was glucose, the OD of the fermentation broth 600nm highest value. It can be seen that compared with other carbon sources (sucrose, lactose), B. licheniformis / B. subtilis has a higher utilization rate of glucose.

[0060] Table 2

[0061]

Embodiment 3

[0062] Embodiment 3, the selection of optimal nitrogen source

[0063] The medium to be tested is composed of TSB broth basal medium containing 1 weight part of nitrogen source (peptone, yeast extract powder, beef extract powder).

[0064] 1. Inoculate 100 μL of the seed solution into a test tube containing 10 mL of the medium to be tested (the inoculation volume is 1% (v / v)), then place it on a constant temperature shaker, and culture it with shaking at 37°C and 200 rpm for 18 hours to obtain fermentation broth.

[0065] 2. After completing step 1, measure the OD of the fermentation broth by ultraviolet spectrophotometry 600nm value.

[0066] The test results are shown in Table 3. The results showed that when the nitrogen source in the medium to be tested was peptone, the OD of the fermentation broth 600nm highest value. It can be seen that compared with other nitrogen sources (yeast extract powder, beef extract powder), Bacillus licheniformis / Bacillus subtilis has a hig...

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Abstract

The invention discloses a bacillus culture medium, which is prepared from the following raw materials: peptone soybean broth, glucose, peptone, sodium dihydrogen phosphate, calcium chloride, biotin and water. According to the bacillus licheniformis culture medium provided by the invention, an OD600nm value of bacillus licheniformis fermentation liquor and the OD600nm value of bacillus subtilis fermentation liquor are respectively increased by 43.3% and 35.1%; the biomass of the bacillus licheniformis/bacillus subtilis is obviously increased; the result shows that the culture medium provided bythe invention is more suitable for the growth of bacillus licheniformis/bacillus subtilis, and the bacillus licheniformis/bacillus subtilis in the optimized culture medium enters a logarithmic phaseafter being fermented for 4 hours, so that the fermentation time can be obviously shortened, and the production cost is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a bacillus culture medium. Background technique [0002] Due to the long-term and large-scale use of antibiotics, the normal intestinal flora of animals is imbalanced, and the residues of antibiotics in livestock and poultry products directly affect human health and the ecological environment. Food safety incidents that have occurred continuously in recent years have caused people to increase their awareness of health and pay attention to green and healthy food. The demand for harmless feed and antibiotic substitutes are urgent problems to be solved. Probiotic preparations are the most promising alternatives to antibiotics, and the physiological efficacy of probiotics has been unanimously recognized in both food and feed. [0003] The "Catalogue of Feed Additives (2013)" issued by the Ministry of Agriculture of my country allows Bacillus lichenifo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/10C12R1/125
CPCC12N1/20
Inventor 钟舒红李军彭昊张美晨潘艳冯世文吴翠兰李常挺贺会利
Owner GUANGXI VETERINARY RES INST
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