Andrias davidianus collagen peptide, preparation method of andrias davidianus collagen peptide, and preparation containing andrias davidianus collagen peptide

A collagen peptide and non-collagen technology, which is applied in the field of deep processing of giant salamander, can solve the problems of lack of target efficacy, indistinguishability, and difficulty in ensuring the content and activity of specific functional peptides

Active Publication Date: 2020-10-16
张兆熙
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The composition of polypeptide products after enzymatic hydrolysis of giant salamander collagen is very complex, including functionally active peptides with known or unknown sequences, but also a large number of peptides that do not have the target effect or even any substantial effect. The existence of these peptides will interfere with The absorption and utilization of functionally active peptides form a competitive relationship, which is not conducive to the absorption and utilization of functiona

Method used

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  • Andrias davidianus collagen peptide, preparation method of andrias davidianus collagen peptide, and preparation containing andrias davidianus collagen peptide

Examples

Experimental program
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Effect test

Embodiment 1

[0066] This embodiment provides a giant salamander collagen peptide, which is prepared by the following method:

[0067] 1. Pretreatment of giant salamander raw materials: after the whole giant salamander fish is cut into pieces and the skin, meat and fat are separated, the skin and meat are soaked in 0.1mol / L sodium hydroxide solution for 4 hours to remove non-collagen protein. The ratio (g:ml) is 1:8, and the NaOH solution is replaced once during soaking; after soaking, wash with deionized water until neutral, drain and set aside;

[0068] 2. Enzymolysis: Put the pretreated giant salamander raw material in step 1 into the enzymolysis tank, add deionized water according to the material-liquid ratio of 1:1.5 (g:ml), start stirring, heat up to 100°C, and keep warm with a slight boil 60min.

[0069] 1) Lower the temperature to 60°C, adjust the pH to 8.0 with calcium hydroxide, add alkaline protease according to 3‰ of the mass of the material, and perform enzymatic hydrolysis fo...

Embodiment 2

[0079] This example provides a giant salamander collagen peptide, the difference between its preparation method and Example 1 is only step 2, and the details of step 2 are as follows:

[0080] Enzymolysis: Put the pretreated giant salamander raw material in step 1 into the enzymolysis tank, add deionized water according to the material-to-liquid ratio of 1:1.5 (g:ml), start stirring, raise the temperature to 100°C, and keep it warm for 60 minutes.

[0081] 1) Cool down to 60°C, adjust the pH to 6.5 with calcium hydroxide, add alkaline protease according to 1‰ of the mass of the material, and perform enzymatic hydrolysis for 1.5 hours, maintaining a pH of 6.0-9.0 during the enzymatic hydrolysis;

[0082] 2) Cool down to 50°C, add neutral protease with a material quality of 1‰ at natural pH, and continue enzymatic hydrolysis for 2.5 hours, maintaining pH 6.0-8.0 during the enzymatic hydrolysis process;

[0083] 3) Add flavor protease with material quality of 1‰, continue enzymol...

Embodiment 3

[0085] This embodiment provides a composition, which consists of the following components by weight: 4 parts of giant salamander collagen peptide in Example 1, and 1 part of vitamin C.

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Abstract

The invention relates to the technical field of deep processing of andrias davidianus, and in particular relates to andrias davidianus collagen peptide, a preparation method of the andrias davidianuscollagen peptide, and a preparation containing the andrias davidianus collagen peptide. Raw materials of andrias davidianus, fat and non-collagen protein of which are removed, are subjected to three-stage enzymolysis of alkaline protease, neutral protease and flavourzyme in sequence; then, enzymolysis substances are filtered and refined to obtain the andrias davidianus collagen peptide; the average molecular weight of the andrias davidianus collagen peptide is below 1000 Da; the protein content is above 95%; the ash content is below 2%; furthermore, the andrias davidianus collagen peptide hasa function of improving skin cell proliferation; the content of small molecule active peptide is high; and thus, the andrias davidianus collagen peptide can excellently promote skin cell proliferativeactivity. The invention further provides a composition containing the andrias davidianus collagen peptide and vitamin C or animal and plant extracts rich in vitamin C, and a preparation thereof; andthe composition and the preparation thereof can improve the skin cell activity and proliferative capability, and effectively relieve skin aging, adermotrophia and the like.

Description

technical field [0001] The invention relates to the technical field of giant salamander deep processing, in particular to a giant salamander collagen peptide, its preparation method, application, composition and preparation containing the giant salamander collagen peptide. Background technique [0002] The giant salamander (Andrias davidianus) is a tailed amphibian belonging to the family Cryptogillidae and the genus Giant salamander, also known as the giant salamander. Collagen is a fibrous protein whose main constituents are amino acids, which are hydrolyzed to produce collagen peptides and by-product amino acids. In recent years, collagen peptides have been increasingly used in various skin care products, health products, medicines and foods due to their significant biological functional activities. The skin and meat of giant salamanders are rich in collagen. In addition to the general collagen peptide's many physiologically active functions such as replenishing water, ...

Claims

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Application Information

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IPC IPC(8): A23L33/18A23L33/105A23L33/125A23L33/15A23L33/16A23L33/175C12P21/06C07K14/78C07K1/34A61K38/01A61K8/65A61P17/00A61Q19/08
CPCA23L33/18A23L33/105A23L33/125A23L33/15A23L33/16A23L33/175C12P21/06C07K14/78A61K38/012A61K8/65A61P17/00A61Q19/08A23V2002/00A23V2200/318A23V2250/55A23V2250/262A23V2250/6402A23V2250/6422
Inventor 张兆熙
Owner 张兆熙
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