Andrias davidianus collagen peptide, preparation method of andrias davidianus collagen peptide, and preparation containing andrias davidianus collagen peptide
A collagen peptide and non-collagen technology, which is applied in the field of deep processing of giant salamander, can solve the problems of lack of target efficacy, indistinguishability, and difficulty in ensuring the content and activity of specific functional peptides
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Embodiment 1
[0066] This embodiment provides a giant salamander collagen peptide, which is prepared by the following method:
[0067] 1. Pretreatment of giant salamander raw materials: after the whole giant salamander fish is cut into pieces and the skin, meat and fat are separated, the skin and meat are soaked in 0.1mol / L sodium hydroxide solution for 4 hours to remove non-collagen protein. The ratio (g:ml) is 1:8, and the NaOH solution is replaced once during soaking; after soaking, wash with deionized water until neutral, drain and set aside;
[0068] 2. Enzymolysis: Put the pretreated giant salamander raw material in step 1 into the enzymolysis tank, add deionized water according to the material-liquid ratio of 1:1.5 (g:ml), start stirring, heat up to 100°C, and keep warm with a slight boil 60min.
[0069] 1) Lower the temperature to 60°C, adjust the pH to 8.0 with calcium hydroxide, add alkaline protease according to 3‰ of the mass of the material, and perform enzymatic hydrolysis fo...
Embodiment 2
[0079] This example provides a giant salamander collagen peptide, the difference between its preparation method and Example 1 is only step 2, and the details of step 2 are as follows:
[0080] Enzymolysis: Put the pretreated giant salamander raw material in step 1 into the enzymolysis tank, add deionized water according to the material-to-liquid ratio of 1:1.5 (g:ml), start stirring, raise the temperature to 100°C, and keep it warm for 60 minutes.
[0081] 1) Cool down to 60°C, adjust the pH to 6.5 with calcium hydroxide, add alkaline protease according to 1‰ of the mass of the material, and perform enzymatic hydrolysis for 1.5 hours, maintaining a pH of 6.0-9.0 during the enzymatic hydrolysis;
[0082] 2) Cool down to 50°C, add neutral protease with a material quality of 1‰ at natural pH, and continue enzymatic hydrolysis for 2.5 hours, maintaining pH 6.0-8.0 during the enzymatic hydrolysis process;
[0083] 3) Add flavor protease with material quality of 1‰, continue enzymol...
Embodiment 3
[0085] This embodiment provides a composition, which consists of the following components by weight: 4 parts of giant salamander collagen peptide in Example 1, and 1 part of vitamin C.
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