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Application of psoralen A in preparation of medicine for preventing or treating cochlear hair cell damage

A technology of psoralen A and cochlear hair cells, which is applied in drug combination, drug delivery, and pharmaceutical formulation to achieve the effect of protecting hearing and preventing ear damage

Active Publication Date: 2022-06-17
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing auricular point therapy only stimulates points through physical means such as "pressure" and "magnetism", and does not combine effective drug percutaneous absorption for treatment

Method used

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  • Application of psoralen A in preparation of medicine for preventing or treating cochlear hair cell damage
  • Application of psoralen A in preparation of medicine for preventing or treating cochlear hair cell damage
  • Application of psoralen A in preparation of medicine for preventing or treating cochlear hair cell damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Evaluation of the effect of psoralen in preventing and protecting cochlear hair cell damage

[0027]Studies have shown that high-intensity noise exposure or exposure to ototoxic drugs can cause apoptosis, degeneration, necrosis, and central auditory processing dysfunction of cochlear hair cells. pathological changes. Among them, the accumulation of reactive oxygen species (ROS) is the cause of hearing impairment. Therefore, after the prevention and protection of psoralen A, the cochlear hair cell (HEI-OC1) injury model after stimulation by the ototoxic drug paracetamol was subjected to MTT cell activity detection, flow cytometric apoptosis detection and intracellular reactive oxygen species ROS. To verify the protective effect of psoralen A on ear injury prevention.

[0028] 1. Materials:

[0029] Preparation of psoralen A stock solution: 0.0050 g of psoralen A was taken and dissolved in 500 μl of dimethyl sulfoxide (DMSO) to prepare a 20 mM psoralen A sto...

Embodiment 2

[0050] Example 2: Preparation of Psoralen A drug-loaded pellets

[0051] 1. Materials

[0052] The carrier is medical stone pellets with a diameter of about 2mm. The scientific name is quartz monzonite (purchased from Hebei Hongyao Mineral Products Company). Inorganic aluminosilicates with high spherical hardness and microporous surface (see figure 2 Shown in A and B), with strong adsorption, it is an ideal framework material for drug-loaded pellets; Opadry (purchased from Shanghai Colorcon Coating Technology Co., Ltd.) is a common drug coating material, It can optimize appearance, improve odor, effectively prevent light and moisture, and increase drug stability.

[0053] 2. Instruments: electronic balance, precision booster electric stirrer, 300ml beaker, fluidized bed (Glatt, Germany).

[0054] 3. Preparation of psoralen A drug-loaded pellets

[0055] (1) Drug loading by fluidized bed liquid lamination method

[0056] Psoralen A coating solution formula 1: psoralen A 0...

Embodiment 3

[0071] Example 3: Preparation of Psoralen Hydrogel

[0072] 1. Quality composition of psoralen A hydrogel:

[0073] Quality composition of psoralen A hydrogel: the final concentration of psoralen A is 0.002%, sodium polyacrylate (AH-105X) 5.4%, aluminum glycerol 0.19%, tartaric acid 0.23%, glycerin 15.77%, polyethylene glycol Alcohol (PEG 400) 4.5%, Sodium Carboxymethyl Cellulose (CMC-NA) 0.33%, Disodium EDTA-2NA 0.25%, Polyvinylpyrrolidone (PVPK90) 0.62%, Azone 0.6% , the balance is deionized water, and the total amount is 100%.

[0074] 2. Preparation process:

[0075] (1) Instruments: electronic balance, precision booster electric stirrer, 300ml beaker, spatula, mortar.

[0076] (2) Preparation method: according to the formula amount, mix 5.4g of sodium polyacrylate, 0.33g of CMC-NA, 0.25g of EDTA-2NA and 0.19g of aluminum glycerol, grind them with a mortar, and then add 15.77g of glycerol, 4.5g PEG400, stir evenly at low speed (preferably without foaming), which is the...

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Abstract

The invention discloses an application of psoralen A in the preparation of a drug for preventing or treating cochlear hair cell damage, the drug is auricular point sticking beans for preventing noise-induced hearing loss; the ear point sticking beans of the invention is psoralen Auricular acupoint sticking beans with psoralen A hydrogel, the psoralen A hydrogel auricular point sticking beans are obtained by pasting psoralen A drug-loaded pellets on psoralen A hydrogel and drying in the shade at room temperature for 24 hours The active ingredient of psoralen A hydrogel auricular point sticking beans of the present invention is derived from the kidney-tonifying traditional Chinese medicine psoralen, which is combined with traditional Chinese medicine auricular point treatment methods to make auricular point sticking beans. After pharmacodynamic verification, the preparation Targeted protection against cochlear cell damage caused by various reasons can not only press and stimulate ear points, but also stably and effectively absorb drugs through the skin to jointly protect hearing.

Description

(1) Technical field [0001] The invention relates to an ear acupoint sticking bean, which can prevent ear damage caused by recreational noise. (2) Background technology [0002] Noise-induced hearing loss, also known as noise-induced deafness, is a slow, progressive sensorineural hearing loss caused by long-term exposure to noise stimuli. Recreational noise damage refers to the ear damage caused by the unreasonable use of mobile phones, MP3 and other audio equipment with headphones, and in KTV, bars and other noisy entertainment places. Studies at home and abroad have proven that prolonged exposure to recreational noise at high volume will cause serious damage to cochlear hair cells. The number of cochlear hair cells in each person is fixed, and they have no regeneration ability. Once lost, it will cause irreversible damage to the user's hearing. Excessive hearing loss can have a profound impact on us both individually and as a society, including physical and functional, so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/352A61K9/06A61K9/50A61K47/32A61K47/38A61K47/10A61P27/16
CPCA61K31/352A61K9/06A61K9/5047A61K9/0046A61K47/32A61K47/38A61K47/10A61P27/16
Inventor 高建莉沈佳曼丁船贺唯易
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY