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Amide type cross-linked polymer, preparation method thereof and application thereof in biological protein precipitation

A cross-linked polymer and amide-type technology, which is applied in the field of biological protein precipitation, can solve the problems of time-consuming denaturation or renaturation, and achieve the effects of improving separation speed, good application prospects, and good thermal stability

Active Publication Date: 2020-10-16
NINGBO AJCORE BIOSCIENCES INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These protein removal methods have three main disadvantages. First, the material removed from the medium is not pure protein. Instead, when the medium contains a complex chemical mixture (such as when the medium is a cell lysate), the use of these methods may cause Causes removal of substances other than protein
Second, proteins removed by these methods are often irreversibly denatured or renatured only through time-consuming procedures such as dialysis

Method used

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  • Amide type cross-linked polymer, preparation method thereof and application thereof in biological protein precipitation
  • Amide type cross-linked polymer, preparation method thereof and application thereof in biological protein precipitation
  • Amide type cross-linked polymer, preparation method thereof and application thereof in biological protein precipitation

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Experimental program
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Effect test

Embodiment 1

[0032] The preparation of embodiment 1 amide type cross-linked polymer

[0033] 1. Sources of raw materials for this application:

[0034] raw material source Copolymer of methyl vinyl ether and maleic anhydride Hubei Xinjing New Material Co., Ltd. 1,2 diaminoimidazole Jiangsu Aikang Biomedical Research and Development Co., Ltd. acetone Sinopharm Chemical Reagent Co., Ltd. hydrochloric acid Sinopharm Chemical Reagent Co., Ltd. sodium hydroxide Sinopharm Chemical Reagent Co., Ltd.

[0035] 2. The reaction scheme of the application experiment is as follows:

[0036]

[0037] Among them, n=200.

[0038] 3. Specific reaction steps

[0039] Methyl vinyl ether and maleic anhydride copolymer (II) and 1,2 diaminoimidazole (III) are respectively dissolved in acetone, the concentration is 0.5 mol / L, according to the copolymer (II) and imidazole based diamine ( III) The molar ratio is 1:2, the acetone solution dissolved with imidaz...

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Abstract

The invention relates to the technical field of new materials for biological protein precipitation, in particular to a diamine dicarboxylic acid cross-linked polymer, a preparation method thereof andapplication of the diamine dicarboxylic acid cross-linked polymer in biological protein precipitation. According to thediamine dicarboxylic acid cross-linked polymer, an imidazolyl diamine is adoptedto replace a common diamine, so that the polymer has better thermal stability and antibacterial property, can greatly facilitate biological detection operation, and is convenient to store and transport in a wider temperature range. Therefore, the production cost of biological detection is reduced.

Description

technical field [0001] The present application relates to the technical field of new materials for biological protein precipitation, in particular to an amide type cross-linked polymer, its preparation method and its use in biological protein precipitation. Background technique [0002] Gene amplification detection is based on polymerase chain reaction (PCR) technology, which is an important analysis method in the field of molecular diagnosis, and its standardization requirements have been paid more and more attention by people. PCR technology has been widely used in clinical disease diagnosis and genetic disease screening. In clinical practice, blood samples are generally used as test samples for quantitative and qualitative analysis of target DNA by PCR technology. Hemoglobin in blood cells has an inhibitory effect on the PCR reaction, so it is often necessary to remove the hemoglobin in the sample in the nucleic acid detection of blood samples. Currently, proteins are tr...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08L35/08C08K5/3445C07K1/30
CPCC08J3/24C07K1/30C08J2335/08C08K5/3445
Inventor 王德明周杰锋
Owner NINGBO AJCORE BIOSCIENCES INC