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Preparation and application of composite gold SERS substrate

A technology for substrate and carbon synthesis, applied in the field of analytical chemistry, can solve the problems of subcutaneous tissue necrosis, long time consumption, low detection limit, etc., and achieve the effects of simple operation, good reproducibility and high sensitivity

Inactive Publication Date: 2020-10-16
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Rhodamine B (Rhodamine B), also known as rose red B, or basic rhodamine, commonly known as flower pink, is a kind of artificially synthesized dye with bright pink color, which is often used as a fluorescent staining agent for cells in laboratories, and has also been used As a food additive, but it can cause necrosis of subcutaneous tissue, it has been banned for food dyeing
However, there is no national detection standard for rhodamine B at present, and the commonly used high-performance liquid chromatography detection method takes a long time, so it is of great significance to establish a platform with high sensitivity, low detection limit, fast detection speed and wide detection range

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Slowly add 0.8 g of graphite and 3.0 g of potassium permanganate into the concentrated sulfuric acid solution, mix them and transfer them to a reaction kettle, place them in a thermostat blast drying oven at 100 ºC for 1.5 h, and add the product A large amount of water and a certain amount of hydrogen peroxide solution were stirred, and the supernatant was discarded, and washed with hydrochloric acid and deionized water for several times until the solution was close to neutral, and graphene oxide GO was obtained.

[0018] (2) Synthesis of nitrogen-doped graphene oxide: Mix the above-prepared graphene oxide solution, ammonia water and deionized water, transfer it to a reaction kettle, and react in an oven at 180 ºC for 12 h to obtain nitrogen Doped graphene oxide NGO.

[0019] (3) Add 20, 40, 60, 80 and 100 ml of 250 mM HAuCl aqueous solution into the boiling carboxylated NGO-COOH suspension, respectively, and stir for 20 min.

[0020] (4) Put the Au@NGO solution sy...

Embodiment 2

[0023] (1) Slowly add 0.5 g of graphite and 2.5 g of potassium permanganate into the concentrated sulfuric acid solution, mix them and transfer them to a reaction kettle, place them in a thermostat blast drying oven at 100 ºC for 1.5 h, and add the product A large amount of water and a certain amount of hydrogen peroxide solution were stirred, and the supernatant was discarded, and washed with hydrochloric acid and deionized water for several times until the solution was close to neutral, and graphene oxide GO was obtained.

[0024] (2) Synthesis of nitrogen-doped graphene oxide: Mix the above-prepared graphene oxide solution, ammonia water and deionized water, transfer it to a reaction kettle, and react in an oven at 180 ºC for 12 h to obtain nitrogen Doped graphene oxide NGO.

[0025] (3) Activation of the above NGO: NaOH (1.2 g) and chloroacetic acid (1 g) were added to the NGO suspension (2 mg / mL) and sonicated for 3 h, OH groups Conversion to COOH groups; neutralization ...

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Abstract

The invention discloses preparation and application of a nitrogen-doped graphene oxide coated gold nanoparticle SERS substrate. The preparation comprises the following steps: synthesizing nitrogen-doped graphene oxide by an improved method, performing in-situ nucleation and growth of gold nanoparticles by virtue of a large specific surface area and a large number of oxygen-containing functional groups on the surface of the nitrogen-doped graphene oxide, and realizing coating; the prepared Au@NGO being subjected to film forming and being transferred to a silicon wafer, and dropwise adding a rhodamine B solution to the silicon wafer for SERS detection and signal acquisition. Compared with common graphene oxide, the nitrogen-doped graphene oxide has higher stability and uniformity, the surface of the nitrogen-doped graphene oxide is more uniform after the nitrogen-doped graphene oxide is coated with the gold nanoparticles, an Au@NGO solution is transferred to the silicon wafer after beingsubjected to film formation, and the stability of an SERS substrate and the reproducibility and reliability of SERS signals are improved.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a SERS substrate and a preparation method thereof. Background technique [0002] The surface-enhanced Raman scattering (SERS) effect refers to that in some specially prepared metal good conductor surfaces or sols, in the excitation region, due to the enhancement of the electromagnetic field on the surface or near the surface of the sample, the Raman scattering signal of the adsorbed molecules is larger than that of the ordinary Raman scattering signal. Mann scattering (NRS) signal greatly enhanced phenomenon. Surface-enhanced Raman spectroscopy can provide rich fingerprint information based on molecular structure, high sensitivity, low detection limit, and even ultra-high sensitivity at the single-molecule level, and is widely used in material characterization, trace analysis detection and biomolecular analysis and many other fields. [0003] Graphene oxide is a promising na...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65B82Y30/00B82Y40/00
CPCG01N21/658B82Y30/00B82Y40/00
Inventor 颜梅郝梦娇张晶冯晓雯魏全勇孙荣环赵悦英于京华
Owner UNIV OF JINAN
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