Preparation method and application of temperature-sensitive antler cartilage matrix hydrogel material

A cartilage matrix, hydrogel technology, applied in medical science, tissue regeneration, prosthesis, etc., can solve problems such as incomplete cell removal

Active Publication Date: 2020-10-20
TAIZHOU UNIV
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Problems solved by technology

[0004] The present invention provides a preparation method and application of a temperature-sensitive antler cartilage matrix hydrogel...

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  • Preparation method and application of temperature-sensitive antler cartilage matrix hydrogel material
  • Preparation method and application of temperature-sensitive antler cartilage matrix hydrogel material
  • Preparation method and application of temperature-sensitive antler cartilage matrix hydrogel material

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specific Embodiment approach 1

[0049] Embodiment 1: The preparation method of a temperature-sensitive velvet cartilage matrix hydrogel material of the present embodiment is carried out according to the following steps:

[0050] 1. Use a vacuum pump to remove the blood in the antler, then cut the antler skin along the longitudinal direction, and tear off the skin;

[0051] 2. Soak the deer antler treated in step 1 in PBS buffer containing 2U / mL aprotinin, and then place it in a closed pressurizing device (deep-sea seabed water pressure experimental device, Jinan Simingte), at a pressure of 100MPa , the temperature is 30℃ and the pressure is maintained for 15min;

[0052] 3. Cut the deer antler processed in step 2 into 5mm thin slices with a slicer, and then grind it with a bone crusher to a particle size of 0.1 mm to obtain deer antler bone mud;

[0053] 4. ① Put the deer antler bone mud and freeze-thaw buffer (10mM Tris-HCl, 1.5wt% Triton X, pH 8) in a metal airtight container according to the ratio of mat...

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Abstract

The invention discloses a preparation method and application of a temperature-sensitive antler cartilage matrix hydrogel material, belongs to the technical field of tissue engineering, and aims to solve the technical problem of incomplete cell removal of an acellular matrix material for antler cartilage at present. The method comprises the following steps of removing blood and skin of pilose antler, soaking the pilose antler in a PBS buffer solution containing aprotinin, pressurizing the pilose antler in a closed pressurizing device, slicing and grinding the pilose antler, mixing the ground pilose antler and a freeze-thaw buffer solution in a metal closed container for incubation, quickly freezing a mixture with liquid nitrogen, unfreezing the mixture, and treating the mixture with trypsindigestive juice, an EDTA decontaminant, a nucleic acid remover and a bacterial remover in sequence, carrying out vacuum freeze drying, and performing digestion by pepsase digestive juice to obtain temperature-sensitive antler cartilage matrix hydrogel. Compared with an existing antler cartilage decellularization treatment method, the treatment method has a better cell removal effect, extracellular matrix components are not seriously lost through decellularization treatment, gaps of a matrix material obtained after decellularization are uniform, and the vascular lumen structure is still reserved.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering; in particular, it relates to a preparation method and application of a temperature-sensitive deer antler cartilage matrix hydrogel material. Background technique [0002] At present, articular cartilage defects caused by trauma and various diseases are very common in clinical practice, but the self-repair ability of articular cartilage is extremely limited. At present, the methods used in the clinical treatment of articular cartilage injury (cartilage, periosteum transplantation, subchondral bone drilling, arthroplasty, etc.) have their limitations, and none of them can achieve long-term cartilage repair. The emergence of tissue engineering methods provides a new material for tissue repair and reconstruction, that is, acellular matrix materials, especially acellular matrix materials for cartilage tissue. However, the current cartilage acellular matrix scaffold is only suitable for the...

Claims

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Application Information

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IPC IPC(8): A61L27/52A61L27/36
CPCA61L27/52A61L27/3608A61L27/3654A61L27/3687A61L27/3691A61L2430/06A61L2430/40
Inventor 褚文辉付永前席晶
Owner TAIZHOU UNIV
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