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Gelatin/gellan gum/hydroxyapatite composite hydrogel and preparation method thereof

A composite hydrogel and hydroxyapatite technology, which is applied in the fields of pharmaceutical formula, medical science, prosthesis, etc., can solve the problems of large cell toxicity, difficulty in complete degradation, unfavorable tissue cell attachment and proliferation, etc.

Active Publication Date: 2020-10-20
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, synthetic polymers are usually accompanied by high cytotoxicity, which is not conducive to the attachment and proliferation of tissue cells, and is not easy to be completely degraded, which poses challenges to the research and application of tissue engineering.

Method used

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  • Gelatin/gellan gum/hydroxyapatite composite hydrogel and preparation method thereof
  • Gelatin/gellan gum/hydroxyapatite composite hydrogel and preparation method thereof
  • Gelatin/gellan gum/hydroxyapatite composite hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Preparation and Characterization of Oxidized Gellan Gum

[0057] One, the preparation method of oxidized gellan gum

[0058] The preparation process of oxidized gellan gum (OG) is as follows:

[0059] (1) Weigh a certain amount of low-acyl gellan gum (GG), disperse it in 200 mL of distilled water at 90 °C, stir for 30 min to obtain a uniform GG solution, and gradually cool down to 40 °C.

[0060] (2) Add a certain amount of NaIO to the GG solution 4 , under the condition of avoiding light, control the oxidation temperature and oxidation time, and vigorously stir to obtain the oxidized gellan gum mixture.

[0061] (3) Pour the oxidized gellan gum mixture into the dialysis bag, the molecular weight of the dialysis bag cut-off molecule is 8-14kDa, dialyze with distilled water at room temperature for 5 days to remove the residual NaIO 4 With reaction by-products, the mixed solution after dialysis is suction-filtered, and the solution obtained by suction filtrat...

Embodiment 2

[0079] Example 2 Preparation and Characterization of Aminated Hydroxyapatite

[0080] 1. Preparation of Aminated Hydroxyapatite

[0081] The preparation process of aminated hydroxyapatite includes the synthesis of hydroxyapatite (Hap) and the process of preparing aminated hydroxyapatite (mHap) from hydroxyapatite. The specific process is as follows:

[0082] (1) Synthesis of hydroxyapatite

[0083] (1) Dissolve calcium nitrate and diammonium hydrogen phosphate in distilled water respectively to obtain 1.2M Ca 2+ solution, 0.72M HPO 4 2- solution.

[0084] (2) At 40°C, the Ca 2+ The solution is slowly dropped into HPO according to the volume ratio of 1:1 4 2- In the solution, at the same time, ammonia water was used to stabilize the pH of the mixed solution at 10.5, and the reaction was continued for 2 hours.

[0085] (3) Let the reaction solution stand at 50°C for 12 hours, collect the white precipitate by centrifugation, wash the precipitate with absolute ethanol and ...

Embodiment 3

[0109] Example 3 Preparation and Characterization of Gelatin / Oxidized Gellan Gum Hydrogel

[0110] One, the preparation method of gelatin / oxidized gellan gum hydrogel

[0111] The gelatin / oxidized gellan gum (Gel-OG) hydrogel was prepared as follows:

[0112] (1) Weigh 1 g of Gel and dissolve it in distilled water at 50°C to prepare a 20 wt% Gel solution.

[0113] (2) Weigh a certain amount of OG and dissolve it in distilled water at 40°C to prepare 1wt%, 1.5wt%, 2wt%, 2.5wt% and 3wt% OG solutions respectively; among them, oxidized gellan gum (OG) Prepared by the conditions optimized in Example 1, that is, GG and NaIO in the preparation process of OG 4 The mass ratio is 1:0.9, the oxidation temperature is 40°C, and the oxidation time is 8h.

[0114] (3) Mix different concentrations of OG solution and Gel solution at a mass ratio of 1:1 at 40°C.

[0115](4) Pour the mixed solution into five glass test tubes, each of which has an inner diameter of 13mm and a depth of 100mm, ...

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Abstract

The invention belongs to the technical field of tissue engineering, and relates to a new material applied to cartilage substitutes, in particular to gelatin / gellan gum / hydroxyapatite composite hydrogel and a preparation method thereof. The composite hydrogel is prepared from gelatin, low-acyl oxidized gellan gum and aminated hydroxyapatite, and the weight ratio of the gelatin to the low-acyl oxidized gellan gum is (10-30): (1-3); wherein the addition amount of the aminated hydroxyapatite is 0.5%-2% of the total weight of the gelatin and the oxidized gellan gum. The particle size of the modified mHap is obviously reduced, which is closer to nanoscale, the distribution of the mHap in the composite hydrogel is more uniform; moreover, after mHap is introduced into Gel-OG, the network of the formed hydrogel is more compact; the introduction of the mHap has a promoting effect on the proliferation of cells, so that the Gel-OG / mHap has good biocompatibility, degradability and self-healing property, and the application of the Gel-OG / mHap composite hydrogel in the field of cartilage tissue engineering is facilitated.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering, and relates to a new material applied to cartilage substitutes, in particular to a gelatin / gellan gum / hydroxyapatite composite hydrogel and a preparation method thereof. Background technique [0002] Cartilage tissue is a highly ordered, fiber-reinforced and cell-attached hydrogel-like tissue, which mainly contains cartilage tissue cells and extracellular matrix (ECM), without lymph, blood vessels and other organs. Cartilage tissue contains about 70% water, 20% collagen and 10% protease, and the tissue is gradually divided into four layers inwardly, namely the surface, middle, deep and calcified cartilage areas, each layer has different physical properties. chemical and biological properties. Cartilage tissue covers the ends of bone joints, and has the functions of decompressing loads and protecting joints from direct mechanical damage. It is very important for the life activities of ...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L89/00C08L5/00C08K9/06C08K3/32A61L27/52A61L27/46A61L27/50A61L27/58
CPCC08J3/075A61L27/52A61L27/46A61L27/50A61L27/58C08J2389/00C08J2405/00C08K9/06C08K3/32A61L2400/06A61L2430/06
Inventor 汤克勇王绿阳李萌雅王芳刘捷郑学晶
Owner ZHENGZHOU UNIV
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