Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of blueberry proliferation medium and its proliferation culture method

A proliferation medium and a technology for proliferation culture, applied in the field of blueberry proliferation medium and its proliferation culture, can solve the problems of blueberry tissue culture seedlings being weak, low transplanting survival rate, low utilization rate, etc., achieve low production cost, improve proliferation coefficient, the effect of increasing utilization

Active Publication Date: 2021-03-05
ZHEJIANG LANMEI AGRI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems in the prior art, to propose a blueberry proliferation medium and its proliferation culture method, which solves the problem of blueberry tissue culture seedlings being thin and weak, with low differentiation rate, long culture period, low utilization rate, and subsequent transplantation. The problem of low planting survival rate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] a) Preparation of culture medium:

[0019] Add 0.8mg / L of ZT (zeatin), 0.08mg / L of IBA (indole butyric acid), 25mg / L of sucrose and 5.5mg / L of agar in the basic medium to obtain the primary culture medium. 0.4 mg / L of ZT, 0.04 mg / L of IBA, 25 mg / L of sucrose and 5.5 mg / L of agar were added to the medium to obtain a subculture medium. Both the primary culture medium and the secondary culture medium are prepared in 5 L, and sterilized for later use.

[0020] Among them, the basic medium includes 675mg / L NH 4 NO 3 , K of 445mg / L 2 SO 4 , 570mg / L of KNO 3 , 280mg / L of MgSO 4 ·7H 2 O, KH of 125mg / L 2 PO 4 , 250mg / L Ca(NO 3 ) 2 4H 2 O, 175mg / L of Cacl 2 2H 2 O, 5mg / L of H 3 BO 3 , 15mg / L of MnSO 4 ·H 2 O, 7.5mg / L ZnSO 4 ·7H 2 O, 0.15mg / L of Na 2 MoO 4 2H 2 O, 0.15mg / L CuSO 4 ·5H 2 O, 35mg / L Na 2 EDTA, FeSO 25mg / L 4 ·7H 2 O, 80mg / L inositol, 0.4mg / L vitamin B 5 , 0.4mg / L of pyridoxine hydrochloride, 0.8mg / L of thiamine hydrochloride and 1.5mg / L of ...

Embodiment 2

[0034] Add 1 mg / L of ZT, 0.1 mg / L of IBA, 30 mg / L of sucrose and 6.5 mg / L of agar in the basic medium to obtain the primary culture medium, add 0.5 mg / L of ZT, 0.05 mg / L of IBA, 30 mg / L of sucrose and 6.5 mg / L of agar were used to obtain the subculture medium. Both the primary medium and the subculture medium were configured in 5 L, and sterilized for use.

[0035] Among them, the basic medium includes 750mg / L NH 4 NO 3 , K of 495mg / L 2 SO 4 , 633mg / L of KNO 3 , 308mg / L of MgSO 4 ·7H 2 O, KH of 142mg / L 2 PO 4 , 278mg / L Ca(NO 3 ) 2 4H 2 O, 195mg / L of Cacl 2 2H 2 O, 6.2 mg / L of H 3 BO 3 , 16.9mg / L of MnSO 4 ·H 2 O, 8.6mg / L ZnSO 4 ·7H 2 O, 0.25mg / L of Na 2 MoO 4 2H 2 O, 0.25mg / L CuSO 4 ·5H 2 O, 37.3mg / L of Na 2 EDTA, 27.8mg / L FeSO 4 ·7H 2 O, 100mg / L inositol, 0.5mg / L vitamin B 5 , 0.5mg / L of pyridoxine hydrochloride, 1mg / L of thiamine hydrochloride and 2mg / L of glycine.

[0036] Others are the same as embodiment one.

Embodiment 3

[0038] Add 1.2mg / L of ZT, 0.12mg / L of IBA, 35mg / L of sucrose and 7.5mg / L of agar in the basic medium to obtain the primary medium, add 0.6mg / L of ZT in the basic medium , the IBA of 0.06mg / L, the sucrose of 35mg / L and the agar of 7.5mg / L thereby obtain subculture medium, primary medium and subculture medium are all configured 5L, sterilized for standby.

[0039] Among them, the basic medium includes 825mg / L NH 4 NO 3 , K of 545mg / L 2 SO 4 , 690mg / L of KNO 3 , 340mg / L of MgSO 4 ·7H 2 O, 155mg / L of KH 2 PO 4 , 310mg / L Ca(NO 3 ) 2 4H 2 O, 215mg / L of Cacl 2 2H 2 O, 7mg / L of H 3 BO 3 , 20mg / L of MnSO 4 ·H 2 O, 9.5mg / L ZnSO 4 ·7H 2 O, 0.35mg / L of Na 2 MoO 4 2H 2 O, 0.35mg / L CuSO 4 ·5H 2 O, 40mg / L Na 2 EDTA, 30mg / L FeSO 4 ·7H 2 O, 120mg / L inositol, 0.6mg / L vitamin B 5 , 0.6mg / L of pyridoxine hydrochloride, 1.2mg / L of thiamine hydrochloride and 2.5mg / L of glycine.

[0040] Others are the same as embodiment one.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses a blueberry proliferation culture medium and method. The blueberry proliferation culture medium includes a primary culture medium and a successive culture medium; the primary culture medium includes a basal culture medium, 0.8-1.2 mg / L ZT, 0.08-0.12 mg / L IBA, 25-35 mg / L sucrose and 5.5-7.5 mg / L agar; and the successive culture medium includes a basal culture medium,0.4-0.6 mg / L ZT, 0.04-0.06 mg / L IBA, 25-35 mg / L sucrose and 5.5-7.5 mg / L agar. The blueberry proliferation culture method includes: a) preparation of a culture medium; b) induction of lateral bud germination; and c) proliferation culture. The prepared culture medium is low in preparation cost; and during use, the survival rate of cultured seedlings reaches 100%, the differentiation rate is 1.5 times as high as that of a commercially available culture medium, the culture period is short, tissue culture seedlings may reach about 8 cm after being cultured for 50 days and have thick and strong stem segments, not only is the utilization ratio of the tissue culture seedlings increased, but also the survival rate of subsequent transplantation is increased; and by using a tissue culture method, the proliferation coefficient may be greatly increased within a short time.

Description

【Technical field】 [0001] The invention relates to the technical field of blueberry multiplication, in particular to the technical field of a blueberry multiplication medium and a method for multiplication and cultivation thereof. 【Background technique】 [0002] Blueberry, belonging to Rhododendronaceae, Vaccinium plant, originated in North America, is a perennial shrub small berry fruit tree. Because blueberry has extremely high economic value and nutritional value, it has attracted people's attention. Blueberries not only contain nutrients such as fructose, fiber and vitamins, but also rich in antioxidants, folic acid and anthocyanins, which help to remove free radicals in the human body, inhibit and reverse human aging, and are listed by the International Food and Agriculture Organization as one of the five major human health one of food. [0003] With the continuous increase in the international demand for blueberries, the cultivation area of ​​blueberries in the world ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005A01H4/008
Inventor 杨姣杨曙方仪传冬王琦赵荣芳洪麒明
Owner ZHEJIANG LANMEI AGRI