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Silk fibroin sericin composite membrane and preparation method of composite membrane

A technology of sericin and composite membranes, which is applied in medical science, tissue regeneration, prostheses, etc. It can solve the problems of poor mechanical properties of regenerated silk protein membranes, large damage to macromolecular structures, and difficulty in meeting replacement requirements.

Active Publication Date: 2020-10-27
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The regenerated silk (silk fibroin, sericin) protein film studied above also has poor mechanical properties, and it is difficult to meet the requirements of hard tissue replacement such as dura mater and dura mater. The macromolecular structure of fibroin and sericin is greatly damaged, and the degree of polymerization (molecular weight) of the macromolecule is an extremely important internal factor determining the performance of the macromolecule

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0027] Aiming at the problems in the prior art that the silk fibroin macromolecules are severely damaged or the silk fibroin sericin composite solution is prepared separately, which affects the toughness and strength of the silk fibroin sericin composite film, the present application provides a silk fibroin sericin composite film, This kind of composite film is prepared from silk fibroin sericin composite solution. In the preparation process of silk fibroin sericin composite solution, a large molecular weight and high viscosity silk fibroin sericin protein composite solution is prepared by using deionized water temperature and degumming, and one-step dissolution method. This method can avoid the destruction of silk fiber macromolecules, and finally make the silk fibroin in the prepared silk fibroin sericin composite solution have higher viscosity and large molecular weight, and then the silk fibroin sericin composite film prepared therefrom Has good toughness and strength. Spe...

Embodiment 1

[0042] 1. Take silkworm raw silk that has been boiled and reeled as raw material, take 1 kg of silkworm raw silk, take 50L of deionized preheated to 60°C according to the bath ratio of 1:50 (g / mL), put the raw silk into the raw silk at a constant temperature of 60°C and stir for treatment 1 to 10 minutes, then take it out and wash the silk with deionization at 25 to 30°C, dehydrate it, and dry it with hot air at 40 to 60°C;

[0043] 2. Put the dried silk in 90°C preheated deionized water according to the bath ratio of 1:50 (g / mL) and cook at a controlled constant temperature for 3 hours, then take out the silk containing sericin and wash it thoroughly with deionized water at 25-30°C , after dehydration, dry with hot air at 40-60°C;

[0044] 3. The degumming rate of silk measured by sampling weighing method is 14%;

[0045] 4. The dried silkworm silk was weighed and dissolved in 9.3M lithium bromide aqueous solution at a bath ratio of 1:25 (g / mL), and treated at 65°C until the...

Embodiment 2

[0050] 1. Take silkworm raw silk that has been boiled and reeled as raw material, take 1 kg of silkworm raw silk, take 50L of deionized preheated to 60°C according to the bath ratio of 1:50 (g / mL), put the raw silk into the raw silk at a constant temperature of 60°C and stir for treatment 1 to 10 minutes, then take it out and wash the silk with deionization at 25 to 30°C, dehydrate it, and dry it with hot air at 40 to 60°C;

[0051] 2. The dried silk is placed in 90°C preheated deionized water according to the bath ratio of 1:50 (g / mL) and boiled at a controlled constant temperature for 5 hours, and the silk containing sericin is taken out and fully cleaned with deionized water at 25-30°C , after dehydration, dry with hot air at 40-60°C;

[0052] 3. The degumming rate of silk measured by sampling weighing method is 21%;

[0053] 4. Weigh the dried silkworm silk and dissolve it in a 9.3M lithium bromide aqueous solution at a bath ratio of 1:25 (g / mL), and treat at 65°C until t...

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Abstract

The invention provides a preparation method of a silk fibroin sericin composite membrane. The method comprises the following steps: (A) putting silk fibroin in warm water for pretreatment, then placing the silk fibroin in water at 65-95 DEG C, and conducting drying to obtain a pre-treated silk; (B) treating the initially-treated silk in a neutral salt solution, then purifying the obtained silk fibroin sericin composite solution, and obtaining a silk fibroin sericin composite solution; and (C) concentrating the silk fibroin sericin composite solution into a film, placing the obtained initial composite film in a small molecule monohydric alcohol for treatment, then conducting washing and drying, and obtaining the silk fibroin sericin composite film. According to the application, the silk fibroin sericin composite solution is prepared by adopting mild degumming and one-step dissolution method, the composite membrane prepared by the silk fibroin sericin composite solution has high strengthand high toughness, and the neck sticking technical problem of insufficient mechanical properties of tissue repair materials derived from natural biopolymers for hard tissue repair such as endocranium and dura mater, is solved.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a silk fibroin sericin composite membrane and a preparation method thereof. Background technique [0002] The natural dura mater and dura mater structure is a relatively strong connective tissue film. The dura mater and dura mater repair materials currently used come from autologous tissue, allogeneic, xenogeneic biological materials and artificial synthetic materials. Product-based, expensive. Autologous tissue and allogeneic transplantation have the best treatment effects, but there are many factors such as limited sources, damage to the donor site, and complications. Domestic clinics often use xenogeneic biological materials such as bovine pericardium, sheep pericardium, porcine peritoneum, mesentery, etc., which have a wide range of sources and low prices. However, due to the processing of xenogeneic antigen proteins, certain toxicity will remain and prevent cel...

Claims

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Application Information

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IPC IPC(8): A61L27/22A61L27/50A61L27/58
CPCA61L27/227A61L27/50A61L27/58A61L2430/32A61L2430/40C08L89/00
Inventor 王建南裔洪根许建梅
Owner SUZHOU UNIV
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