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Chitosan-human recombinant collagen electrostatic spinning nanofiber scaffold and preparation method

A recombinant collagen, electrospinning technology, applied in electrospinning, conjugated synthetic polymer man-made filament, cellulose/protein conjugated man-made filament, etc., can solve the problem of high failure rate, expensive, autologous bone transplantation Material source defects, etc., to achieve the effect of remarkable effect, good fiber morphology, good biocompatibility and biodegradability

Inactive Publication Date: 2020-10-27
青海创铭医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the repair effect of autologous bone graft is better than that of allograft, there are serious defects in the material source of autologous bone graft.
Bone extraction from autologous heterotopic patients is prone to complications after surgery, and the failure rate is as high as 10-30%, which seriously restricts its wide application in clinical practice; allogeneic bone transplantation is quite difficult, expensive, and easy in terms of material selection and storage. Produce immune rejection, may carry some pathogens, cause the occurrence of body diseases, cause certain damage to the body, etc., and the failure rate is higher

Method used

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  • Chitosan-human recombinant collagen electrostatic spinning nanofiber scaffold and preparation method
  • Chitosan-human recombinant collagen electrostatic spinning nanofiber scaffold and preparation method
  • Chitosan-human recombinant collagen electrostatic spinning nanofiber scaffold and preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation method of chitosan-human recombinant collagen electrospinning nanofiber scaffold, specifically:

[0020] Calculated by mass percentage, human recombinant collagen is 8%, chitosan is 2.5%, polycaprolactone is 30%, and hexafluoroisopropanol is 59.5%. The three components of human recombinant collagen, chitosan and polycaprolactone were prepared into hexafluoroisopropanol spinning solution, the spinning solution was sucked into the syringe, and the syringe was placed on the electrospinning machine for electrospinning , the spinning voltage was 11kV, the distance between the needle and the receiving plate was 18cm, the propulsion speed of the propulsion pump was 0.5ml / h, and the spinning ambient temperature was 35°C, the electrospun nanofiber scaffold was prepared, and then passed through Aldehyde steam cross-linked for 3 hours to obtain the chitosan-human recombinant collagen electrospun nanofiber scaffold.

[0021] In the experiment, the receiving device wa...

Embodiment 2

[0024] A preparation method of chitosan-human recombinant collagen electrospinning nanofiber scaffold, specifically:

[0025] Calculated by mass percentage, human recombinant collagen is 10%, chitosan is 4%, polycaprolactone is 25%, and hexafluoroisopropanol is 61%. The three components of human recombinant collagen, chitosan and polycaprolactone were prepared into hexafluoroisopropanol spinning solution, the spinning solution was sucked into the syringe, and the syringe was placed on the electrospinning machine for electrospinning , the spinning voltage was 12kV, the distance between the needle and the receiving plate was 20cm, the propulsion speed of the propulsion pump was 0.6ml / h, and the spinning ambient temperature was 38°C. Electrospinning nanofiber scaffolds were prepared by electrospinning technology, and then crosslinked by glutaraldehyde steam for 3 hours to obtain chitosan-human recombinant collagen electrospinning nanofiber scaffolds.

[0026] figure 2 It is a ...

Embodiment 3

[0029] A preparation method of chitosan-human recombinant collagen electrospinning nanofiber scaffold, specifically:

[0030] Calculated by mass percentage, human recombinant collagen is 10%, chitosan is 3%, polycaprolactone is 30%, and hexafluoroisopropanol is 57%. The three components of human recombinant collagen, chitosan and polycaprolactone were prepared into hexafluoroisopropanol spinning solution, the spinning solution was sucked into the syringe, and the syringe was placed on the electrospinning machine for electrospinning , the spinning voltage was 13kV, the distance between the needle and the receiving plate was 23cm, the propulsion speed of the propulsion pump was 0.8ml / h, and the spinning ambient temperature was 40°C. Electrospinning nanofiber scaffolds were prepared by electrospinning technology, and then crosslinked by glutaraldehyde steam for 3 hours to obtain chitosan-human recombinant collagen electrospinning nanofiber scaffolds.

[0031] Wherein, the molecu...

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Abstract

The invention relates to the technical field of tissue engineering and biological manufacturing, in particular to a chitosan-human recombinant collagen electrostatic spinning nanofiber scaffold and apreparation method. The specific preparation method comprises the following steps: preparing a hexafluoroisopropanol spinning solution with a certain concentration from three components, namely humanrecombinant collagen, chitosan and polycaprolactone according to a certain ratio; and preparing chitosan, humanized recombinant collagen and polycaprolactone electrostatic spinning nanofiber scaffoldsfrom the prepared solution through an electrostatic spinning technology, then cross-linking the nanofiber scaffolds through a cross-linking agent, and applying the cross-linked scaffolds to cartilagetissue regeneration. The chitosan-human recombinant collagen electrostatic spinning nanofiber scaffold prepared by the invention has high fiber morphology, high biocompatibility and biodegradability,has the capability of guiding cartilage regeneration, is remarkable in effect, and can be used for repairing cartilage tissue defects.

Description

technical field [0001] The invention relates to the fields of tissue engineering and biomanufacturing, and the specific field is a nanofiber scaffold. Background technique [0002] There are more than 3 million patients with bone defects or dysfunction in my country every year. Bone injury and bone defect are very common clinically, and at present, autologous bone or allogeneic bone graft is usually used for repair. Although the repair effect of autologous bone graft is better than that of allograft, there are serious defects in the material source of autologous bone graft. Bone extraction from autologous heterotopic patients is prone to complications after surgery, and the failure rate is as high as 10-30%, which seriously restricts its wide application in clinical practice; allogeneic bone transplantation is quite difficult, expensive, and easy in terms of material selection and storage. Produce immune rejection, may carry some pathogens, cause the occurrence of body dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/24A61L27/20A61L27/18A61L27/50D01F8/02D01F8/18D01F8/14D06M13/123D01D5/00D06M101/32
CPCA61L27/24A61L27/20A61L27/18A61L27/50D01F8/02D01F8/18D01F8/14D06M13/123D01D5/003D01D5/0061A61L2430/06A61L2400/12D06M2101/32
Inventor 莫秀梅冯文浩朱世辉盛嘉隽樊璐露高佩
Owner 青海创铭医疗器械有限公司
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