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Application of vitamin D3 to aquaculture

A technology for aquaculture and vitamins, applied in application, fish farming, climate change adaptation, etc., can solve the problems of drug-resistant Vibrio, antibiotic abuse, overuse, etc., to reduce the generation of drug-resistant bacteria and promote the expression level , the effect of improving the ability of infection

Active Publication Date: 2020-11-06
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of bacterial diseases of prawns caused by Vibrio mainly relies on antibiotics, but the abuse or overuse of antibiotics in the aquaculture industry has brought about serious environmental pollution and the emergence of drug-resistant Vibrio.

Method used

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  • Application of vitamin D3 to aquaculture
  • Application of vitamin D3 to aquaculture
  • Application of vitamin D3 to aquaculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] The prawns were divided into two groups, the experimental group and the control group, each group was divided into 6 subgroups, and each subgroup had 10 P. japonicus. In the experimental group, each prawn was orally infected with 50 μL of Vibrio parahaemolyticus V.parahaemolyticus (about 1×10 8 CFU); in the control group, each prawn was orally fed with the same volume of 50 μL sterile PBS solution (2mM KH 2 PO 4 ,10mMNa 2 HPO 4 , 2.7mM KCl, 137mM NaCl, pH 7.4). After 24 hours, the gut contents of each prawn in the subgroup were collected, pooled, and immediately frozen in liquid nitrogen. Finally, 30 mg of intestinal contents were taken from each subgroup and submitted to the company for LC / MS metabolome analysis to analyze the small molecule metabolites in the intestinal contents of prawns. The small molecular metabolites in the experimental group orally infected with Vibrio parahaemolyticus and the control group fed with sterilized PBS were compared, and the diff...

Embodiment 2

[0107] Dissolve commercial vitamin D3 in ethanol at a concentration of 10 mg / mL, and dilute it 500 times in sterile PBS at a concentration of 20 μg / mL. Penaeus japonicus were divided into two groups with 35 prawns in each group. The experimental group was fed with vitamin D3, and each prawn was fed with 1 μg (50 μL, 20 μg / mL) of vitamin D3; the control group was fed with sterilized PBS, and each prawn was fed with 50 μL of PBS. After 24 hours, each prawn was orally infected with Vibrio parahaemolyticus (1×10 8 CFU). Then count the number of dead prawns every day. The experimental group fed vitamin D3 was compared with the control group fed sterilized PBS. After feeding vitamin D3 to prawns, feeding Vibrio parahaemolyticus to prawns can increase the survival rate of prawns by 20-30%.

Embodiment 3

[0109] Penaeus japonicus were divided into two groups with 10 prawns in each group. The experimental group was fed with vitamin D3, and each prawn was fed with 1 μg (50 μL, 20 μg / mL) of vitamin D3; the control group was fed with sterilized PBS, and each prawn was fed with 50 μL of PBS. After 24 hours, each prawn was orally infected with Vibrio parahaemolyticus (1×10 8 CFU). After 6 hours, the stomach and intestinal tract of the prawns were dissected, and the contents in the stomach and intestinal tract were separated, diluted with sterilized PBS, and spread on LB plates (1% NaCl, 1% peptone, 0.5% yeast extract , 1.5% agar, pH 7.0), count the number of colonies on the plate. After feeding vitamin D3 to prawns, oral infection with Vibrio parahaemolyticus can promote the clearance of bacteria in the digestive tract of prawns and reduce the number of Vibrio parahaemolyticus in the stomach and intestines of prawns by 50-70%.

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Abstract

The invention provides an application of vitamin D3 to aquaculture, and belongs to the technical field of aquaculture. According to the vitamin D3 screened based on metabonomics, the fatality rate ofvibrio pathogens to prawns can be reduced, the removal efficiency of intestinal flora of the prawns is improved, and the expression level of antibacterial peptide in digestive tracts of the prawns ispromoted. The application of the vitamin D3 to bacterial disease resistance of the prawns does not cause generation of drug-resistant vibrio, does not cause the problem of environmental pollution, hasno adverse effect on the normal life activity of the prawns, and can improve the vibrio infection resistance of the prawns by adding the vitamin D3 as an immunopotentiator into a prawn feed. The useof antibiotics and pollution to the environment are reduced, and the generation of drug-resistant bacteria in the environment is reduced, so that the vitamin D3 has a good practical application value.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to the application of vitamin D3 in aquaculture. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Shrimp aquaculture is an important pillar industry of marine aquaculture in my country, and occupies an important economic position in aquatic product aquaculture in coastal areas of China. However, the epidemic outbreak of shrimp diseases has brought serious economic losses to my country's aquaculture industry, such as early death syndrome / acute hepatopancreatic necrosis syndrome caused by Vibrio parahaemolyticus and hepatopancreatic necrosis of shrimp caused by Nodam...

Claims

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Application Information

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IPC IPC(8): A01K61/13A01K61/59A23K20/174A23K50/80
CPCA01K61/13A01K61/59A23K50/80A23K20/174Y02A40/81
Inventor 王金星石秀贞杨明冲
Owner SHANDONG UNIV
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