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Application of vitamin d3 in aquaculture

A vitamin and aquatic product technology, applied in the application field of vitamin D3 in aquaculture, can solve the problems of drug-resistant vibrio, antibiotic abuse, overuse, etc., to reduce the generation of drug-resistant bacteria, promote the expression level, improve The effect of the ability to infect

Active Publication Date: 2022-07-01
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of bacterial diseases of prawns caused by Vibrio mainly relies on antibiotics, but the abuse or overuse of antibiotics in the aquaculture industry has brought about serious environmental pollution and the emergence of drug-resistant Vibrio.

Method used

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  • Application of vitamin d3 in aquaculture
  • Application of vitamin d3 in aquaculture
  • Application of vitamin d3 in aquaculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] The prawns were divided into two groups, the experimental group and the control group, each group was divided into 6 subgroups, and each subgroup had 10 P. japonicus. In the experimental group, each shrimp was orally infected with 50 μL of V. parahaemolyticus (about 1×10 8 CFU); in the control group, each shrimp was orally fed the same volume of 50 μL sterile PBS solution (2 mM KH 2 PO 4 ,10mMNa 2 HPO 4 , 2.7 mM KCl, 137 mM NaCl, pH 7.4). After 24 hours, the gut contents of each shrimp in the collected subgroup were mixed and immediately frozen in liquid nitrogen. Finally, 30 mg of intestinal contents were taken from each subgroup and submitted to the company for LC / MS metabolome analysis to analyze the small molecule metabolites in the intestinal contents of shrimp. The experimental group infected with Vibrio parahaemolyticus orally and the control group fed with sterilized PBS were compared with the small molecule metabolites in shrimp, and the differential small...

Embodiment 2

[0107] Commercially available vitamin D3 was dissolved in ethanol at a concentration of 10 mg / mL, and diluted 500 times into sterile PBS at a concentration of 20 μg / mL. Penaeus japonicus was divided into two groups of 35 prawns each. The experimental group was fed with vitamin D3, and each shrimp was fed with 1 μg (50 μL, 20 μg / mL) of vitamin D3; the control group was fed with sterilized PBS, and each shrimp was fed with 50 μL of PBS. After 24 hours, each prawn was orally infected with V. parahaemolyticus (1×10 8 CFU). The number of dead prawns was then counted every day. The experimental group fed with vitamin D3 was compared with the control group fed with sterilized PBS. After feeding prawns with vitamin D3, feeding prawns with Vibrio parahaemolyticus can improve the survival rate of prawns by 20-30%.

Embodiment 3

[0109] Penaeus japonicus was divided into two groups of 10 prawns each. The experimental group was fed with vitamin D3, and each shrimp was fed with 1 μg (50 μL, 20 μg / mL) of vitamin D3; the control group was fed with sterilized PBS, and each shrimp was fed with 50 μL of PBS. After 24 hours, each prawn was orally infected with V. parahaemolyticus (1×10 8 CFU). After 6 hours, the stomach and intestines of prawns were dissected and separated, and the contents in the stomach and intestines were separated. After gradient dilution with sterilized PBS, they were spread on LB plates (1% NaCl, 1% peptone, 0.5% yeast extract). , 1.5% agar, pH 7.0), count the number of colonies on the plate. After feeding prawns with vitamin D3, oral infection of prawns with Vibrio parahaemolyticus can promote bacterial clearance in the digestive tract of prawns and reduce the number of Vibrio parahaemolyticus in the stomach and intestines of prawns by 50-70%.

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Abstract

The invention provides the application of vitamin D3 in aquaculture, and belongs to the technical field of aquaculture. Based on the vitamin D3 screened out by metabolomics, the invention can reduce the lethality of Vibrio pathogens to prawns, improve the clearance efficiency of prawn intestinal flora, and promote the expression level of antimicrobial peptides in the digestive tract of prawns. The application of vitamin D3 in the anti-bacterial disease of prawns will not lead to the production of drug-resistant Vibrio, will not bring about environmental pollution problems, and has no adverse effect on the normal life activities of prawns. Use this vitamin D3 as an immune enhancer Adding it to shrimp feed can improve the ability of shrimp to resist Vibrio infection, reduce the use of antibiotics and environmental pollution, and reduce the production of drug-resistant bacteria in the environment, so it has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, in particular to the application of vitamin D3 in aquaculture. Background technique [0002] The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Shrimp aquaculture is an important pillar industry of marine aquaculture in China, and occupies an important economic position in aquatic product aquaculture in my country's coastal areas. However, the epidemic outbreak of shrimp diseases has brought serious economic losses to my country's aquaculture industry, such as early death syndrome / acute hepatopancreatic necrosis syndrome caused by Vibrio parahaemolyticus and shrimp hepatopancreatic necrosis disease / Shrimp diseases such ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/045A01K61/13A01K61/59A23K50/80A23K20/174
CPCA01K61/13A01K61/59A23K50/80A23K20/174Y02A40/81
Inventor 王金星石秀贞杨明冲
Owner SHANDONG UNIV
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