Application of vitamin d3 in aquaculture
A vitamin and aquatic product technology, applied in the application field of vitamin D3 in aquaculture, can solve the problems of drug-resistant vibrio, antibiotic abuse, overuse, etc., to reduce the generation of drug-resistant bacteria, promote the expression level, improve The effect of the ability to infect
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Embodiment 1
[0105] The prawns were divided into two groups, the experimental group and the control group, each group was divided into 6 subgroups, and each subgroup had 10 P. japonicus. In the experimental group, each shrimp was orally infected with 50 μL of V. parahaemolyticus (about 1×10 8 CFU); in the control group, each shrimp was orally fed the same volume of 50 μL sterile PBS solution (2 mM KH 2 PO 4 ,10mMNa 2 HPO 4 , 2.7 mM KCl, 137 mM NaCl, pH 7.4). After 24 hours, the gut contents of each shrimp in the collected subgroup were mixed and immediately frozen in liquid nitrogen. Finally, 30 mg of intestinal contents were taken from each subgroup and submitted to the company for LC / MS metabolome analysis to analyze the small molecule metabolites in the intestinal contents of shrimp. The experimental group infected with Vibrio parahaemolyticus orally and the control group fed with sterilized PBS were compared with the small molecule metabolites in shrimp, and the differential small...
Embodiment 2
[0107] Commercially available vitamin D3 was dissolved in ethanol at a concentration of 10 mg / mL, and diluted 500 times into sterile PBS at a concentration of 20 μg / mL. Penaeus japonicus was divided into two groups of 35 prawns each. The experimental group was fed with vitamin D3, and each shrimp was fed with 1 μg (50 μL, 20 μg / mL) of vitamin D3; the control group was fed with sterilized PBS, and each shrimp was fed with 50 μL of PBS. After 24 hours, each prawn was orally infected with V. parahaemolyticus (1×10 8 CFU). The number of dead prawns was then counted every day. The experimental group fed with vitamin D3 was compared with the control group fed with sterilized PBS. After feeding prawns with vitamin D3, feeding prawns with Vibrio parahaemolyticus can improve the survival rate of prawns by 20-30%.
Embodiment 3
[0109] Penaeus japonicus was divided into two groups of 10 prawns each. The experimental group was fed with vitamin D3, and each shrimp was fed with 1 μg (50 μL, 20 μg / mL) of vitamin D3; the control group was fed with sterilized PBS, and each shrimp was fed with 50 μL of PBS. After 24 hours, each prawn was orally infected with V. parahaemolyticus (1×10 8 CFU). After 6 hours, the stomach and intestines of prawns were dissected and separated, and the contents in the stomach and intestines were separated. After gradient dilution with sterilized PBS, they were spread on LB plates (1% NaCl, 1% peptone, 0.5% yeast extract). , 1.5% agar, pH 7.0), count the number of colonies on the plate. After feeding prawns with vitamin D3, oral infection of prawns with Vibrio parahaemolyticus can promote bacterial clearance in the digestive tract of prawns and reduce the number of Vibrio parahaemolyticus in the stomach and intestines of prawns by 50-70%.
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