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Glycoside compound derived from raw medicinal materials, extraction method and application thereof

A compound and glycoside technology, applied in the field of glycoside compounds, can solve the problems of drug stability and effectiveness to be improved, easy to breed microorganisms, unfavorable storage and transportation of drug dosage control, etc., to achieve a significant effect of reducing uric acid

Active Publication Date: 2020-11-06
西藏甘露藏药股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the raw extracts extracted in the prior art are troublesome to store, easy to breed microorganisms, and easy to deteriorate, which is not conducive to storage and transportation and the control of the dosage, and the stability and effectiveness of the medicine need to be improved.

Method used

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  • Glycoside compound derived from raw medicinal materials, extraction method and application thereof
  • Glycoside compound derived from raw medicinal materials, extraction method and application thereof
  • Glycoside compound derived from raw medicinal materials, extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] Embodiment 1: a kind of raw and so on dry ointment:

[0084] Such as figure 2 with image 3 As shown, the dry paste of raw materials is prepared through the following steps:

[0085] (1) After cleaning and slicing 20kg of raw and other medicinal materials, add 120L of water to a 500L sandwich pot for the first decoction. The decoction temperature is 190°C and the decoction time is 90min. Stir once every 20min. , stirred at 15r / min for 1min; after decocting, filter for the first time (100 mesh sieve) to obtain medicinal residues I and medicinal liquid I;

[0086] (2) Add 80 L of water to the medicinal dregs I, and decoct for the second time. The decoction temperature is 190° C., the decoction time is 120 min, stirred once every 20 min, and stirred at 15 r / min for 1 min; after decoction, filter for the second time ( 100 mesh sieve), to obtain medicinal residues II and medicinal liquid II, and discard medicinal residues II;

[0087] (4) Mixing the medicinal liquid I a...

Embodiment 2

[0103] Embodiment 2: a kind of glycoside compound:

[0104] On the basis of Example 1, the glycoside compound was prepared by using the raw and other dry paste obtained, the specific steps are as follows:

[0105] 1) Take the raw raw paste described in Example 1, add 10 times the amount of an aqueous solution containing 65% ethanol and 1% glycerol, mix well, heat up to 85°C, extract 3 times, each time for 3h, and combine the extracts Concentrate under reduced pressure until there is no alcohol smell, and then sequentially extract with petroleum ether (60-90°C), chloroform, ethyl acetate, and water-saturated n-butanol;

[0106] 2) Take the part of ethyl acetate in step 1), separate it by column chromatography on silica gel (200-300 mesh), and elute with chloroform-methanol-water gradient, with volume ratios of 9:1:1 and 8:2: 1. The mixed solution of 7:3:1, 6:4:1, and 5:5:1 is eluted, and the eluted fractions 1 to 5 are collected correspondingly;

[0107] 3) Take fraction 5 (t...

Embodiment 3～12

[0111] Embodiment 3~12: a kind of glycoside compound:

[0112] On the basis of Example 1, the glycoside compound was prepared by using the raw and other dry paste obtained, the specific steps are as follows:

[0113] 1) Take the raw and other dry extracts described in Example 1, add 10 times the amount of aqueous solution, mix uniformly and then heat up to 85 ° C, extract 3 times, each time for 3 hours, combine the extracts, concentrate under reduced pressure, and then use petroleum ether ( 60~90℃), chloroform, ethyl acetate, and water-saturated n-butanol for extraction;

[0114] 2) with embodiment 2;

[0115] 3) with embodiment 2;

[0116] 4) with embodiment 2;

[0117] 5) Same as Example 2, finally separate and obtain the glycoside compound; wherein

[0118] The components of the aqueous solution of step 1) are specifically shown in Table 2.

[0119] The alcohol extraction solution composition in table 2, embodiment 2~12

[0120]

[0121] Statistical embodiment 2~12...

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Abstract

The invention relates to the technical field of glycoside compounds, and particularly relates to a glycoside compound derived from raw medicinal materials, an extraction method and application thereof. The glycoside compound is a carbohydrate derivative containing a carbon ring, and the structural formula of the glycoside compound is shown as a formula (I). The preparation method comprises the steps that the raw medicinal materials are decocted with water, and liquid medicine is filtered and concentrated to obtain extract; the extract is decocted and concentrated to obtain a dry extract semi-finished product, and the dry extract semi-finished product is taken out after standing and dried to obtain raw dry extract; and the raw dry extract is extracted with an alcohol solvent, and separationis conducted through macroporous resin, reversed phase column chromatography and preparative high performance liquid chromatography in sequence to obtain the glycoside compound. The glycoside compound derived from the raw medicinal materials has a significant uric acid reducing effect, the high blood uric acid level of an animal model mouse can be restored to a normal level only through a low dosage, proliferation of hepatoma carcinoma cells can be effectively inhibited, and therefore the glycoside compound can be applied to a pharmaceutical preparation for inhibiting proliferation of the hepatoma carcinoma cells.

Description

technical field [0001] The invention relates to the technical field of glycoside compounds, in particular to a glycoside compound derived from raw materials, an extraction method and its application. Background technique [0002] Sheng etc. is generally understood as the dry xylem (called core material) of the stem of Rhamnella gilgitica Mansf. et Melch., a plant of the genus Rhamnella gilgitica Mansf. et Melch. , "Shengdeng", "Sangdang" and so on. The original plant grows in mountainous shrubs or sparse forests at an altitude of 2600-2900m, and is mainly distributed in southeastern Tibet, northwestern Yunnan, and southwestern Sichuan. Can cool blood, hold back dry yellow water, reduce swelling. Indications for rheumatoid arthritis, leprosy, alpine plethora, external application of boiled ointment can reduce swelling and treat sores. "Tara's Materia Medica": can restrain yellow water; "Four Medical Classics": cool blood, restrain yellow water; Arthritis, yellow water dis...

Claims

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Application Information

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IPC IPC(8): C07H15/203C07H1/08A61K31/7034A61K36/72A61P19/06A61P35/00
CPCA61K36/72A61K2236/331A61K2236/51A61P19/06A61P35/00C07H1/08C07H15/203
Inventor 周凤旦增扎西平措南加达瓦加布尼玛曲珍
Owner 西藏甘露藏药股份有限公司
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