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Production method of trehalose

A production method and trehalose technology, applied in the field of sugar engineering, can solve the problems of low trehalose yield, large trehalose loss, large loss, etc., improve the purity and yield of trehalose, improve separation and purification effect, and content loss reduced effect

Active Publication Date: 2020-11-17
JIANGSU OGO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Activated carbon column chromatography can separate the disaccharides and monosaccharides in the enzymatic hydrolysis solution, but cannot effectively remove the oligosaccharides, and this method has a large loss of trehalose, and requires a large amount of organic solvents, which is costly. Not suitable for industrial production
However, the recovery rate of trehalose in the ion exchange resin method is only about 50%, the yield of trehalose is low, and the loss is large

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] This embodiment provides a method for producing trehalose, comprising the following steps:

[0043] Stir water at 50-60°C and add starch, adjust the pH to 6.0-6.5, and control the solid content at 20%-35%; use 40-50u / 1g of α-amylase to catalyze the hydrolysis and liquefaction reaction with the product of step 1), The reaction temperature is 85-98°C, and the reaction time is 10-60min; then the obtained liquefied starch solution is sprayed and liquefied, and the liquefaction temperature is 125°C; the liquefied reaction solution is heated to 80-90°C, kept for 30min, and cooled to 55°C -65°C, then add glucoamylase for enzymolysis reaction, the amount of glucoamylase added is 20U / ml, adjust the pH to 4.0-6.0, control the saccharification temperature at 55-65°C, enzymolysis time is 2h, add maltooligosaccharide-based seaweed Sugar synthase, maltooligosaccharide-based trehalose hydrolase and trehalose synthase were enzymatically transformed for 12 hours to obtain saccharificati...

Embodiment 2

[0046] This embodiment provides a method for producing trehalose, which includes the following steps: stir water at 50-60°C and add starch, adjust the pH to 6.0-6.5, and control the solid content at 20%-35%; use 40-50u / 1g α-amylase and the product of step 1) carry out catalytic hydrolysis liquefaction reaction, the reaction temperature is 85-98°C, and the reaction time is 10-60min; then the obtained liquefied starch solution is sprayed and liquefied, and the liquefaction temperature is 125°C; the liquefied Heat the reaction liquid to 80-90°C, keep it warm for 25min, cool down to 55-65°C, then add glucoamylase for enzymolysis reaction, the amount of glucoamylase added is 15U / ml, adjust the pH to 4.0-6.0, and control the saccharification temperature at 55 -65°C, enzymatic hydrolysis time is 2.5h, add maltooligosaccharide-based trehalose synthase, maltooligosaccharide-based trehalose hydrolase and trehalose synthase for enzymatic conversion for 12h, and obtain saccharification cru...

Embodiment 3

[0049]This embodiment provides a method for producing trehalose, which includes the following steps: stir water at 50-60°C and add starch, adjust the pH to 6.0-6.5, and control the solid content at 20%-35%; use 40-50u / 1g α-amylase and the product of step 1) carry out catalytic hydrolysis liquefaction reaction, the reaction temperature is 85-98°C, and the reaction time is 10-60min; then the obtained liquefied starch solution is sprayed and liquefied, and the liquefaction temperature is 125°C; the liquefied Heat the reaction liquid to 80-90°C, keep it warm for 30min, cool down to 55-65°C, then add glucoamylase for enzymolysis reaction, the amount of glucoamylase added is 20U / ml, adjust the pH to 4.0-6.0, and control the saccharification temperature at 55 -65°C, enzymatic hydrolysis time is 3h, add maltooligosaccharide-based trehalose synthase, maltooligosaccharide-based trehalose hydrolase and trehalose synthase for enzymatic conversion for 12h, and obtain saccharified crude liqu...

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Abstract

The invention provides a production method of trehalose. The production method comprises the steps of starch gelatinization, liquefaction, saccharification, enzyme deactivation, protein removal, decoloration, primary membrane separation, chromatographic separation, hydrogenation reaction, secondary membrane separation, concentration and crystallization, recrystallization, drying, crushing, screening, packaging and warehousing. According to the production method of trehalose disclosed by the invention, various enzymes are matched, part of maltose generated after enzymolysis of glucoamylase is converted into trehalose, and glucose, oligosaccharide and trehalose in sugar liquid are separated step by step; and by carrying out hydrogenation reaction on the separated oligosaccharide liquid containing a large amount of oligosaccharide and a small amount of trehalose, and trehalose sugar liquid containing a large amount of trehalose and a small amount of oligosaccharide respectively, the oligosaccharide liquid and oligosaccharide in the trehalose liquid are subjected to hydrogenation reaction to generate sugar alcohol; and the sugar alcohol and the trehalose are separated respectively, then the sugar alcohol and the trehalose are concentrated and crystallized, and finally recrystallization and purification are performed respectively, so that the content loss of the trehalose is reduced, the yield is increased, and the separation and purification effects are improved.

Description

technical field [0001] The invention relates to the technical field of sugar engineering, in particular to a method for producing trehalose. Background technique [0002] Trehalose is a non-reducing disaccharide formed by combining two glucose molecules through α, α-1,1 bonds. It widely exists in bacteria, fungi, algae, lower plants and insects. It has been found through research that this sugar has unique biological functions, such as protecting biological macromolecules, protecting cell membranes, and protecting proteins from damage caused by freezing, drying, and changes in osmotic pressure. It is widely used in food, medicine, cosmetics, agriculture, etc. application. [0003] At present, the production methods of trehalose mainly include microbial extraction, fermentation, enzyme conversion and genetic engineering. Among them, the enzymatic conversion method has broad application prospects because of its low cost, simple process, and high conversion rate. However, i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12C12P19/18C12P19/14C07H1/06C07H3/04
CPCC12P19/12C12P19/18C12P19/14C07H1/06C07H3/04
Inventor 楼志华于令君李莉
Owner JIANGSU OGO BIOTECH
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