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A kind of fermentation method of coenzyme q10

A fermentation method and coenzyme technology, applied in the field of microbial fermentation, can solve the problem of low fermentation units

Active Publication Date: 2022-03-08
NORTH CHINA PHARMA COMPANY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As described in a Chinese invention patent with the publication number CN102168115, a coenzyme Q10 industrial production method: by adding key storage factors, controlling the content of dissolved phosphorus, controlling the content of amino nitrogen, adding 3 times concentrated fermentation base material, etc. to increase coenzyme Q10 The fermentation unit reaches 3400mg / L; the Chinese invention patent with the publication number CN104561154 describes a coenzyme Q10 fermentation process and control strategy: the transition period of the bacterial form in the fermentation process is used as the time point for process parameter adjustment, and the fermentation process is adjusted. The ventilation rate of the tank, the pressure of the tank and the stirring speed and other methods increase the fermentation unit of coenzyme Q10, and the fermentation unit reaches 3500mg / L; the Chinese invention patent with the publication number of CN105483171 describes a production method for increasing the industrial output of coenzyme Q10: by controlling the seed The concentration of ferrous ions in the culture medium increases the fermentation unit of coenzyme Q10, and the fermentation unit reaches 3382mg / L. It can be seen that the fermentation technology of coenzyme Q10 is relatively mature, but the existing industrial production methods of coenzyme Q10 produced by microbial fermentation all have partial fermentation units. low problem

Method used

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  • A kind of fermentation method of coenzyme q10

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Seed culture: dilute the coenzyme Q10 production strain in the glycerol tube to an appropriate concentration and inoculate it on the eggplant bottle slant medium, and place the slant in an incubator with a temperature of 32±0.5°C and a relative humidity of 40%-60% Cultured for 6 days. After the eggplant bottle slant culture is completed, use an inoculation needle to pick 3 colonies and inoculate them into a 500mL triangular flask containing 60mL of medium, and place the mother bottle at a temperature of 32±0.5°C, a relative humidity of 40%-60%, After cultivating in a shaker at 220rpm for 30h, inoculate the primary seed tank as the mother bottle seed. The formula of eggplant bottle slant medium is: glucose 2g / L, yeast powder 1.5g / L, peptone 0.5g / L, sodium chloride 1.5g / L, agar powder 15g / L, pH6.85, sterilized at 121±0.5℃ 20min. Mother bottle medium formula is: glucose 5g / L, yeast powder 0.4g / L, peptone 0.3g / L, corn steep liquor 0.7g / L, ammonium sulfate 1.2g / L, monosodi...

Embodiment 2

[0039] Seed culture: dilute the coenzyme Q10 production strain in the glycerol tube to an appropriate concentration and inoculate it on the eggplant bottle slant medium, and place the slant in an incubator with a temperature of 32±0.5°C and a relative humidity of 40%-60% Cultured for 5 days. After the eggplant bottle slant culture is completed, use an inoculation needle to pick 4 colonies and inoculate them into a 500mL triangular flask containing 60mL of medium, and place the mother bottle at a temperature of 32±0.5°C, a relative humidity of 40%-60%, After cultivating in a shaker at 220rpm for 28h, inoculate the primary seed tank as the mother bottle seed. The formula of eggplant bottle slant medium is: glucose 3g / L, yeast powder 1.6g / L, peptone 0.4g / L, sodium chloride 1.5g / L, agar powder 15g / L, pH6.80, sterilized at 121±0.5℃ 20min. Mother bottle medium formula is: glucose 4g / L, yeast powder 0.5g / L, peptone 0.2g / L, corn steep liquor 0.6g / L, ammonium sulfate 1.3g / L, monosodi...

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Abstract

The invention discloses a fermentation method of coenzyme Q10, which belongs to the field of microbial fermentation. In the method, the concentration of ammonium ions is controlled periodically, lysine salt is added to the fermentation medium, and lysine salt is added again during the fermentation process. Sodium sulfate was added once. This fermentation control process significantly enhances the growth and metabolism of coenzyme Q10-producing bacteria, significantly increases the amount of bacteria, and the fermentation unit of coenzyme Q10 increases rapidly. Collected by filtration, the batch yield of coenzyme Q10 is greatly increased, which significantly reduces the fermentation cost of coenzyme Q10.

Description

technical field [0001] The invention relates to a fermentation method of coenzyme Q10, which belongs to the field of microbial fermentation. Background technique [0002] Coenzyme Q10 (Ubiquinone-10) is a naturally occurring fat-soluble quinone compound, also known as decenoquinone, ubiquinone-10, chemical name: 2,3-dimethoxy-5-methyl-6- Decyl-1,4-benzoquinone (2,3-dimethoxy-5-methyl-6-decaprenyl-1,4-benzoquinone), the molecular formula is: C 59 h 90 o 4 , the molecular weight is: 863.37, and the structural formula is: [0003] [0004] The compound is a yellow to orange crystalline powder, odorless, tasteless, soluble in benzene, acetone, and ether, almost insoluble in water and methanol, unstable under direct sunlight, coenzyme Q10 is widely distributed in nature, mainly in In the cells of yeast, plant leaves, seeds and animals' hearts, livers and kidneys, especially in the biofilms rich in mitochondria in mammals, the content is higher in the hearts, livers, kidney...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/66C12N1/38C12N1/20C12R1/01
CPCC12P7/66C12N1/38C12N1/20
Inventor 段志钢王平尹贵超王成王淑琳张军剪王云牛霄亮李景李默晗高雨宁丁宝娟
Owner NORTH CHINA PHARMA COMPANY
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