Micro-fluidic chip, kit and application method of kit

Abstract

The invention relates to a micro-fluidic chip, a kit and an application method of the kit. A base body of the micro-fluidic chip is provided with a positioning groove, a sample cavity, a lysis solution storage cavity, a sample lysis cavity, a cleaning solution storage cavity, an eluent storage cavity, a buffer cavity, a nucleic acid cavity, a waste liquid cavity, a distribution pipeline and at least two PCR reaction cavities; and the base body is further provided with a protease solution storage cavity, a mixing cavity, a filtering area and a selection cavity. The number of PCR reaction cavities can be set when the chip is applied to detection; a fluorescent PCR detection method is combined with a micro-fluidic technology, the advantages of the two technologies are complementary, the defects of the two technologies are avoided, the possibility of leakage of samples or chemical reagents is avoided, the possibility of nucleic acid pollution is greatly reduced, related pathogenic bacteriain the samples can be quickly and accurately identified, the kit is particularly suitable for realizing multiple detection, and the experimental time and cost are saved; and the requirements on operation environment, personnel skills and the like are low, the automation degree is high, the consumed time is short, and the performance indexes such as specificity and sensitivity of detection can beconsidered.

Description

technical field [0001] This application relates to the field of germ detection, in particular to a microfluidic chip, a kit and an application method thereof. Background technique [0002] Pathogenic bacteria refer to microorganisms that can cause human diseases, also known as pathogenic microorganisms. Generally speaking, pathogenic bacteria refer to bacteria in pathogenic microorganisms. Food-borne pathogenic bacteria refer to pathogenic bacteria introduced during food processing and distribution, and are bacteria that are transmitted through food. These pathogenic bacteria survive in food, grow and metabolize food and cause deterioration and destruction of food. At the same time, some pathogenic bacteria secrete toxic substances. It can cause common infectious diseases and food poisoning in humans or animals. Common pathogenic bacteria in food include Salmonella, Enterobacter sakazakii, Shigella, Campylobacter jejuni, Staphylococcus aureus, Vibrio parahaemolyticus, Liste...

AI Technical Summary

Problems solved by technology

[0005] Therefore, the traditional detection method has problems such as complex operation, long time, low accuracy and low stability. In addition, there is no microfluidic chip on the market that can simultaneously detect multiple foodborne pathogens

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