Micro-fluidic chip, kit and application method of kit

A technology of microfluidic chips and storage chambers, which is applied in chemical instruments and methods, biochemical equipment and methods, and laboratory containers, etc., and can solve problems such as complex operations, low accuracy, and low stability

Pending Publication Date: 2020-12-01
深圳市刚竹医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Therefore, the traditional detection method has problems such as complex operation, long time, low accuracy and low st

Method used

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  • Micro-fluidic chip, kit and application method of kit
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  • Micro-fluidic chip, kit and application method of kit

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0033] Example 1

[0034] Primer probe combination shown in Table 2 below:

[0035] FAM channel HEX channel ROX channel 1 reaction chamber salmonella Shigella Internal standard No. 2 reaction chamber Staphylococcus aureus Single plus Listeria Internal standard No. 3 reaction chamber Crowth arc O1 group Monocho O139 group Internal standard No. 4 reaction chamber Vibrosisolysis Invigorate Internal standard No. 5 reaction chamber Monopstool Cellula Internal standard No. 6 reaction chamber Campylobacter jejuni Colon curved bacteria Internal standard No. 7 reaction chamber E. coli O157 Escherichia coli O104 Internal standard No. 8 reaction chamber Pseudonic Small intestinal colitis Yerssen Internal standard No. 9 reaction chamber Welding Gas Single Gas capsia Internal standard 10th reaction chamber Housuma Bacillus wax Internal standard

[0036] Table 2

[0037] The kit of operation and results of th...

Example Embodiment

[0042] Example 2

[0043] Detecting samples with 21 micro-fluidic chip, including food-borne pathogens 20 positive samples and one negative samples. Sample No. 1 Salmonella positive samples, sample No. 2 as Shigella positive samples, sample No. 3 Staphylococcus aureus positive samples, 4 samples Listeria monocytogenes positive samples, 5 samples Vibrio cholerae O1 positive sample group, sample No. 6 as Vibrio cholerae O139 serogroup positive samples, 7 samples positive samples Vibrio parahaemolyticus, Vibrio vulnificus 8 samples positive samples, the sample No. 9 is mimicus positive samples, sample No. 10 Vibrio alginolyticus is positive samples, 11 samples jejuni positive samples, 12 samples Campylobacter positive samples, 13 samples of E. coli O157 positive samples, 14 samples of E. coli O104 positive samples No. 15 Yersinia pseudotuberculosis samples positive samples, 16 samples Yersinia enterocolitica positive samples, 17 samples Aeromonas hydrophila positive samples, 18 sampl...

Example Embodiment

[0048] Example 3

[0049] Microfluidic chip using the mixed sample detection of foodborne pathogens plasmid the 20, serially diluted samples of plasmid 105copies / ml, 104copies / ml, 103copies / ml, 102copies / ml, 101copies / ml in a certain concentration gradient five concentrations, the sensitivity test. Detecting operational flow according to Example 1 of the embodiment, the detection results are shown in Table 4.

[0050]

[0051]

[0052] Table 4

[0053] The detection results show that the microfluidic kit of 20 kinds of foodborne pathogenic bacteria is highly sensitive to all 20 kinds of food-derived pathogenic bacteria, and it has reached 100 copies / ml.

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Abstract

The invention relates to a micro-fluidic chip, a kit and an application method of the kit. A base body of the micro-fluidic chip is provided with a positioning groove, a sample cavity, a lysis solution storage cavity, a sample lysis cavity, a cleaning solution storage cavity, an eluent storage cavity, a buffer cavity, a nucleic acid cavity, a waste liquid cavity, a distribution pipeline and at least two PCR reaction cavities; and the base body is further provided with a protease solution storage cavity, a mixing cavity, a filtering area and a selection cavity. The number of PCR reaction cavities can be set when the chip is applied to detection; a fluorescent PCR detection method is combined with a micro-fluidic technology, the advantages of the two technologies are complementary, the defects of the two technologies are avoided, the possibility of leakage of samples or chemical reagents is avoided, the possibility of nucleic acid pollution is greatly reduced, related pathogenic bacteriain the samples can be quickly and accurately identified, the kit is particularly suitable for realizing multiple detection, and the experimental time and cost are saved; and the requirements on operation environment, personnel skills and the like are low, the automation degree is high, the consumed time is short, and the performance indexes such as specificity and sensitivity of detection can beconsidered.

Description

technical field [0001] This application relates to the field of germ detection, in particular to a microfluidic chip, a kit and an application method thereof. Background technique [0002] Pathogenic bacteria refer to microorganisms that can cause human diseases, also known as pathogenic microorganisms. Generally speaking, pathogenic bacteria refer to bacteria in pathogenic microorganisms. Food-borne pathogenic bacteria refer to pathogenic bacteria introduced during food processing and distribution, and are bacteria that are transmitted through food. These pathogenic bacteria survive in food, grow and metabolize food and cause deterioration and destruction of food. At the same time, some pathogenic bacteria secrete toxic substances. It can cause common infectious diseases and food poisoning in humans or animals. Common pathogenic bacteria in food include Salmonella, Enterobacter sakazakii, Shigella, Campylobacter jejuni, Staphylococcus aureus, Vibrio parahaemolyticus, Liste...

Claims

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Application Information

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IPC IPC(8): C12M1/34C12M1/00
CPCB01L3/5027Y02A50/30
Inventor 游腾飞
Owner 深圳市刚竹医疗科技有限公司
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