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Black phosphorus colorimetric/photo-thermal dual-mode visual immunochromatographic detection method for rapid detection of norfloxacin

A technique of fluorescence immunochromatography and norfloxacin, which is applied in the measurement of color/spectral properties, thermal analysis of materials, fluorescence/phosphorescence, etc., can solve the problems of being easily affected by the external environment and the detection accuracy needs to be improved, and achieves the improvement of The effect of accuracy

Pending Publication Date: 2020-12-04
INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current fluorescence immunochromatography is mostly a single fluorescence reading, which is easily affected by the external environment, and the detection accuracy needs to be improved

Method used

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  • Black phosphorus colorimetric/photo-thermal dual-mode visual immunochromatographic detection method for rapid detection of norfloxacin
  • Black phosphorus colorimetric/photo-thermal dual-mode visual immunochromatographic detection method for rapid detection of norfloxacin
  • Black phosphorus colorimetric/photo-thermal dual-mode visual immunochromatographic detection method for rapid detection of norfloxacin

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preparation example Construction

[0036] In the specific implementation process of the present invention, the black phosphorus nanosheets do not need to be coated in advance, and the black phosphorus nanosheets are added 10 minutes after the dropwise sample reaction; the preparation method of the black phosphorus nanosheets preferably includes:

[0037] Mix the oxygen-free N-methylpyrrolidone aqueous solution and the black phosphorus crystal powder, after the black phosphorus crystal is stripped by ultrasonic liquid phase, centrifuge for the first time with 3500~4500rpm, collect the supernatant (remove the unstripped BP crystal residue in the precipitation), and use The supernatant is centrifuged at 15000-18000 rpm for the second time, and the precipitate is collected to obtain black phosphorus nanosheets.

[0038] In the present invention, the concentration of N-methylpyrrolidone in the oxygen-free N-methylpyrrolidone aqueous solution is preferably 3-5mg / 8ml, more preferably 1mg / ml; the solvent of the oxygen-f...

Embodiment 1

[0055] 1. Referring to the core-shell-shell magnetic upconversion composite microspheres provided by CN 109507419 A, prepare magnetic upconversion nanoparticles Fe@Fe 3 o 4 @UCP.

[0056] 2. Magnetic upconversion nanoparticle surface carboxylation modification of Fe@Fe 3 o 4 @UCP

[0057] 10 mL of absolute ethanol with a mass fraction of 1% PAA and 5 mL of 1% Fe@Fe 3 o 4 The chloroform dispersion of @UCP was mixed in a 50 mL one-necked flask. The mixed solution was stirred overnight at room temperature, magnetically separated, and washed three times with absolute ethanol to obtain carboxyl-modified Fe@Fe 3 o 4 @UCP. Carboxyl-modified Fe@Fe will be obtained 3 o 4 @UCP was dispersed in PBS to a final concentration of 0.5 mg / ml.

[0058] 3. Characterization of Magnetic Upconversion Nanoparticles

[0059] The synthesized particles have good dispersibility in cyclohexane. The morphology, elements and carboxyl modification of the particles were characterized by transmiss...

Embodiment 2

[0063] 1. Preparation of BP nanosheets by liquid phase exfoliation method. Dissolve 20 mg of NMP in 40 mL of ultrapure water, and blow with argon for 15 min to fully remove oxygen. Then accurately weighed 20 mg of BP crystal powder was added thereto. The mixed solution was ultrasonicated for 24h (ultrasonic power is 500W) to exfoliate BP crystals in liquid phase. During the ultrasonic process, the water temperature was kept below 25°C. After the stripping is completed, centrifuge at 4000r / min for 10min to remove unstripped BP crystal residues in the precipitate. Then the supernatant was centrifuged at 16000r / min for 10min, and the supernatant was removed to retain the precipitate. The pellet was washed three times with ultrapure water to remove excess NMP on the surface. Finally, the obtained hydrophilic few-layer BP nanosheets were dispersed in ultrapure water and stored at 4°C for future use.

[0064] 2. Observe the morphology of the prepared BP by transmission electron...

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Abstract

The invention provides a black phosphorus colorimetric / photo-thermal dual-mode visual immunochromatographic detection method for rapid detection of norfloxacin, and belongs to the technical field of immunochromatographic test strip detection. A test strip adopted by the detection method comprises a bottom plate, and a sample adding pad, a combination pad, a nitrocellulose membrane and a water absorption pad which are sequentially adhered to the bottom plate, wherein the nitrocellulose membrane comprises a detection area and a quality control area; and black phosphorus nanosheets are dropwise added into the sample adding pad. The BP nanosheet dropwise added into the sample adding pad is used as a colorimetric / photo-thermal display agent. Colorimetric / photo-thermal data can be obtained by collecting and analyzing heat at the T line, and the detection accuracy is improved through the colorimetric / photo-thermal double-reading method.

Description

technical field [0001] The invention relates to the technical field of immunochromatographic test strip detection, in particular to a black phosphorus colorimetric / photothermal dual-mode visual immunochromatographic detection method for rapid detection of norfloxacin. Background technique [0002] Fluorescence immunochromatography is a new type of membrane detection technology based on antigen-antibody specific immune response. In this technology, the strip-shaped fiber chromatography material immobilized with detection lines (coated antibodies or coated antigens) and quality control lines (anti-antibodies) is used as the stationary phase, the test solution is used as the mobile phase, and fluorescently labeled antibodies or antigens are fixed on the connection pads. , to move the analyte across the strip by capillary action. The current fluorescence immunochromatography is mostly a single fluorescence reading, which is easily affected by the external environment, and the d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/577G01N33/558G01N25/48G01N21/78G01N21/25
CPCG01N33/558G01N33/9446G01N21/64G01N25/4833G01N33/549G01N33/533G01N21/78G01N33/5302G01N33/54388G01N33/54387G01N33/582G01N33/577G01N21/25G01N25/48G01N2021/7759
Inventor 周焕英任舒悦高志贤王永辉李双白家磊
Owner INST OF ENVIRONMENTAL MEDICINE & OCCUPATIONAL MEDICINE ACAD OF MILITARY MEDICINE ACAD OF MILITARY SCI
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