Method for quickly breaking dormant buds of fraxinus mandshurica tissue culture seedlings and successfully achieving in-vitro propagation

Abstract

The invention relates to a method for quickly breaking dormant buds of fraxinus mandshurica tissue culture seedlings and successfully achieving in-vitro propagation. According to the method, fraxinusmandshurica tissue culture seedlings with dormant bud points or stems with dormant buds are used as materials, WPM is used as a basic culture medium, TDZ with different concentrations is added, two illumination conditions (light and dark) and two culture manners (solids and liquid) are adopted, the temperature is 25 DEG C, culture is carried out within 7 d, the dormant state of the dormant buds offraxinus mandshurica is relieved, the dormant buds start to germinate and are cultured for 24 d, the germination rate of solid culture reaches 57.14% at most, the germination rate of liquid culture reaches 100% at most, in strong seedling and multiplication culture, culture is carried out for 30 d, the survival rate of an A6 culture medium is 100%, the average seedling length is 1.68 cm, the survival rate of a Z1 culture medium is 75%, and the average seedling height is 2.65 cm. According to the method, the dormant buds of fraxinus mandshurica can be quickly broken to achieve in-vitro propagation, the method can be cyclically used and is a sustainable circulating culture production method, the method is single in hormone, low in concentration and simple in process, the method has the characteristics of controllability and low cost, the method is suitable for different production conditions, and technical support is provided for large-scale propagation of fraxinus mandshurica seedlings.

Description

technical field [0001] The invention belongs to the technical field of rapid propagation of improved plant varieties, in particular to a method for quickly breaking dormant buds of Mandshurica mandshurica tissue culture seedlings and successfully in vitro propagation. Background technique [0002] Fraxinus mandshurica Rupr. is a deciduous tree belonging to the family Oleaceae. It is an important and precious broad-leaved tree species in the eastern region, with a wide distribution range. It is mainly distributed in the Xiaoxing'an Mountains, Changbai Mountains, eastern mountains of Liaoning, Japan's Hokkaido, Honshu, and the Korean Peninsula. It grows rapidly, has excellent material, moderate strength, and high wood utilization value. It is a famous military material and high-grade furniture material. However, the resources of this tree species have always been very poor, coupled with long-term irrational over-cutting and utilization, its resources have been exhausted. As...

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Problems solved by technology

At the same time, seed propagation has the disadvantages of being affected by the size of the year of fruiting; while the regeneration of sprouting tillers is restricted by factors such as the presence or absence of felled roots, quantity, and harvesting season. Cutting propagation shows great differences due to the age of the plucked mother tree. Therefore, Researchers began to study the expansion of Mandshurica mandshurica from the perspective of tissue culture

[0003] At present, there are some reports on the tissue culture of Mandshurica mandshurica, but no matter which material (mature embryo, somatic embryo, callus, and axillary bud of annual branch, etc.) is used as the explant, the obtained tissue culture plantlet , there will be dormancy of terminal buds and axillary buds during the cultivation process. With the increase of subculture, the branches of tissue culture seedlings will be lignified, the bud points will be dormant, and the leaves will gradually age and fall off (such as figure 1 ), which has seriously affected the approach of using tissue culture technology to propagate Mandshurica mandshurica plants, which is also a bottleneck that current Mandshurica mandshurica tissue culture propagation cannot be industrialized

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