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Application of folium perillae extracts in preparation of medicine for treating and/or preventing inflammatory bowel disease

A technology of perilla leaf extract and inflammatory bowel disease, which is applied in the field of perilla leaf extract, can solve problems such as water and sodium retention, aggravation of ulcer disease, and elevated blood sugar, and achieve easy standardized production, simple preparation process, and toxic and side effects low effect

Active Publication Date: 2020-12-15
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, aminosalicylic acid, glucocorticoids and immunosuppressants are used clinically for treatment, and these therapeutic drugs are often accompanied by adverse reactions such as exacerbation of ulcer disease, high blood pressure, elevated blood sugar, osteoporosis, water and sodium retention, and decreased blood potassium.
At present, there is no relevant research report on perilla leaf extract and its treatment of inflammatory bowel disease

Method used

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  • Application of folium perillae extracts in preparation of medicine for treating and/or preventing inflammatory bowel disease
  • Application of folium perillae extracts in preparation of medicine for treating and/or preventing inflammatory bowel disease
  • Application of folium perillae extracts in preparation of medicine for treating and/or preventing inflammatory bowel disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: the preparation of perilla leaf extract

[0042] Take 1.5 kg of dried perilla leaves, add 5 liters of 70% ethanol, heat to reflux for 1.5 hours, and filter while hot. The filter residue was treated once more in the same way; the filtrates were combined twice, and the ethanol was recovered under reduced pressure to obtain 65 g of extract. The extract was added with 300mL of water and stirred to form a suspension, which was extracted twice with 150mL of ethyl acetate respectively. The remaining water was dissolved by adding 1580 mL of 95% ethanol, precipitated at room temperature for 24 hours, filtered under reduced pressure, and the filter residue was dissolved in 50 mL of water, freeze-dried and crushed to obtain perilla leaf extract.

Embodiment 2

[0043] Embodiment 2: dosage form preparation

[0044] Capsules: Perilla leaf extract 100g, add dextrin 147.5g, magnesium stearate 2.5g, mix well, make 1000 capsules according to capsule preparation method, fill in 250mg capsules, each capsule contains perilla leaf extract 100mg , and its total amount is not less than 50%.

[0045] Tablets: Take 100g of perilla leaf extract, add 100g of starch, 80g of dextrin, and 15g of sucrose, stir well, make granules, dry below 60°C, add appropriate amount of magnesium stearate and talc, mix well, Compressed into 1000 tablets, coated with sugar or film, that is.

[0046] Granules: Take 100g of perilla leaf extract, add appropriate amount of sucrose and dextrin, make granules according to the granule preparation method, and dry to make 1000g.

[0047] Oral solution: Take perilla leaves, heat and reflux with 70% ethanol for 1.5 hours to extract twice, filter, combine the filtrate, recover ethanol under reduced pressure; add the same amount ...

experiment example 1

[0049] Experimental example 1: Inhibitory effect of perilla leaf extract on DSS-induced inflammatory bowel disease (IBD) in mice

[0050] Methods: SPF grade male C57BL / 6 mice, 18-20g, were randomly divided into normal control group, model control group, positive control group (sulfasalazine, 20mg / kg, intragastric administration), drug treatment group (purple Perilla leaf extract 50, 100mg / kg, intragastric administration), 10 rats in each group. Mice freely drank drinking water containing 2.8% (W / V) DSS for 7 days to prepare a mouse enteritis model. Animals were administered orally one day before model building, once a day for 10 consecutive days. Record the daily body weight, diarrhea, and blood in the stool. On the 11th day, the mice were killed, and the colon of the mice was dissected to measure the length and weight of the colon; the colon was routinely fixed and embedded for HE staining; the biochemical index MPO was measured after the colon was homogenized, and the supe...

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Abstract

The invention discloses application of folium perillae extracts in preparation of a medicine for treating and / or preventing the inflammatory bowel disease. The folium perillae extracts disclosed by the invention have a reverse effect on diarrhea, hematochezia, weight loss and the colon position pathological injuries of a mouse with inflammatory bowel disease induced by low molecular weight dextransulfate, can obviously inhibit secretion of model mouse colon tissue cell factors NO, IFN-gamma, IL-4, IL-10 and IL-17, and can obviously inhibit the intestinal tissue MPO level of the model mouse.

Description

technical field [0001] The invention relates to a perilla leaf extract, a preparation method thereof, and an application of the preparation in medicine or food for preventing and / or treating inflammatory bowel disease, belonging to the technical field of medicine or food. Background technique [0002] Inflammatory bowel disease (IBD) is a type of chronic non-specific intestinal inflammation, mainly including ulcerative colitis and Crohn's disease. Its etiology and pathogenesis have not yet been elucidated. It is a series of pathophysiological processes caused by the participation of inflammatory cells and the inflammatory mediators released by the body under the action of external factors, such as chemicals, infections, and antigens. The disease is characterized by intestinal inflammation and mucosal tissue damage, mainly manifested as diarrhea, abdominal pain, weight loss, and abdominal mass. Its pathological mechanism is closely related to the normal intestinal flora and a...

Claims

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Application Information

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IPC IPC(8): A61K36/535A61P1/00A61P29/00
CPCA61K36/535A61P1/00A61P29/00A61K2236/333
Inventor 林明宝马培姚春所侯琦张梓倩白金叶吉腾飞李姝仪袁继巧
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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