Cadmium ion detection fluorescent probe based on purine parent as well as preparation method and application thereof
A fluorescent probe and cadmium ion technology, applied in the field of fluorescent probes, can solve the problems of expensive testing, long measurement time, and complicated sample pretreatment, and achieve the effects of strong cell penetration, short response time, and low toxicity
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Embodiment 1
[0047] Fluorescent probes for detecting cadmium ions based on purine precursors were prepared by the following method:
[0048] (1) Preparation of intermediate II (6-chloro-N4-(naphthalene-1-yl)pyrimidine-4,5-diamine)
[0049] In a 100 mL round bottom flask, add 5-amino-4,6-dichloropyrimidine (5.00 g, 30 mmol) and 1-naphthylamine (8.58 g, 60 mmol) and dissolve in 50 mL of methanol, then add 5 mL of 12 mol / L HCl . The mixture was stirred at reflux for 5 days at 65°C. After the reaction solution was cooled, the organic solvent was removed by distillation under reduced pressure. Then all the obtained crude products were dissolved in 50 mL of 1 mol / L NaOH solution, and the mixture was extracted 3 times with ethyl acetate, and the ethyl acetate was removed by distillation under reduced pressure. The organic phase was washed with 1.2 mol / L HCl, then dehydrated with saturated brine and dried to obtain a crude product. Crude product with CH 3 OH / H 2 O (v / v, 1:5) recrystallized a...
Embodiment 2
[0067] The detection cadmium ion fluorescence probe that embodiment 1 makes is configured into the probe stock solution of 1mM with EtOH, and each metal ion is configured into the metal ion stock solution of 3mM with deionized water, to the blank solution EtOH-H of 3mL 2 Add 30 μL of probe stock solution and 50 μL of metal ion stock solution to O (v / v=9:1) and detect with a fluorescence spectrometer and an ultraviolet spectrophotometer. The test shows that the maximum excitation wavelength of the fluorescent probe is 396 nm, the maximum The emission wavelength is 532nm, and the specific test results are as follows:
[0068] Take two cuvettes, add 3mL blank solution EtOH-H 2 O (v / v=9:1) to neutralize 30 μL of the probe stock solution, add 50 μL of the cadmium ion stock solution to one of the cuvettes, and not add the cadmium ion stock solution to the other cuvette for UV spectrum testing . Experimental results such as figure 1 As shown, the fluorescent probe compound itself ...
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