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Preparation method and application of microfluidic chip constructed based on silver microsphere monolithic column

A microfluidic chip, monolithic column technology, applied in chemical instruments and methods, laboratory containers, measuring devices, etc., can solve the problems of limited detection sensitivity, uneven distribution of silver microspheres, and complicated preparation process. , to achieve the effect of improved detection accuracy and sensitivity, low production cost and good reproducibility

Active Publication Date: 2020-12-18
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The preparation process of this technology is cumbersome and complicated, and at the same time it brings a lot of organic interference, the distribution of silver microspheres is not uniform, and the content is limited, the detection sensitivity is greatly limited

Method used

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  • Preparation method and application of microfluidic chip constructed based on silver microsphere monolithic column
  • Preparation method and application of microfluidic chip constructed based on silver microsphere monolithic column
  • Preparation method and application of microfluidic chip constructed based on silver microsphere monolithic column

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Effect test

Embodiment 1

[0047] This embodiment provides a preparation method for constructing a microfluidic chip based on a monolithic column of silver microspheres, including:

[0048] S1: Prepare a silver microsphere solution. The surface of the silver microspheres in the silver microsphere solution has nano-silver particles and a nano-pore structure, which can provide Raman detection hotspots.

[0049] S2: Prepare a chip with a microfluidic channel, the microfluidic channel has a Raman detection area for filling the silver microsphere solution, and also serves as an enrichment area and a detection area for the sample to be tested;

[0050] S3: The silver microsphere solution is injected into the Raman detection area of ​​the microfluidic channel chip, and the monolithic column is obtained through natural deposition. Silver microspheres are used instead of molecular sieves as the filler of the monolithic column, and an integrated system with collection and detection functions is obtained. Detectio...

Embodiment 2

[0053] The present embodiment provides a kind of preparation method of silver microsphere, comprises the following steps:

[0054] S10: Using bovine serum albumin BSA as a template and ascorbic acid as a reducing agent, silver nitrate is reduced to prepare silver microspheres.

[0055] Prepare 1 mL of AgNO with a concentration of 150umol / L 3 The solution, the ascorbic acid (Ascorbic Acid, AA) solution, and the bovine serum albumin (BSA) solution with a concentration of 0.5 mg / mL were all deionized water as solvents and were freshly prepared before the reaction.

[0056] S11: Add 17.625mL of deionized water and 375uL of BSA solution to the reaction flask, place the reaction flask on a magnetic stirrer heated to 30°C, set the stirring speed at 600rpm, and put it into the reaction flask after 5 minutes of reaction Add 1mL of prepared AgNO 3 solution, keeping the temperature and stirring speed unchanged for 30 minutes, then adjust the speed to 700rpm in advance, and quickly add ...

Embodiment 3

[0060] This embodiment provides a method for preparing a chip with a microfluidic channel, designing and using PDMS to prepare a monolithic column chip, including the following steps:

[0061] S20: Use CAD to draw a chip sketch, process it into a mask according to the sketch, and use photolithography to obtain a chip template, pour a layer of PDMS colloid on the chip template, place it in an oven at 75 degrees Celsius and bake for 1-2 hours, and wait for the PDMS to solidify for backup use.

[0062] S21: Put the solidified PDMS and the purchased quartz chip into the plasma cleaning machine for 2 minutes, then quickly take them out, and glue them together to form a microfluidic channel, and obtain a chip with a microfluidic channel. refer to Figure 4a As shown, it is a design drawing of a chip with a microfluidic channel. It can be seen from the figure that a curved microfluidic channel 1 is formed in the chip, and the microfluidic channel 1 is provided with a sample injectio...

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Abstract

The invention provides a preparation method and application of a microfluidic chip constructed based on a silver microsphere monolithic column; the preparation method comprises the following steps: preparing a silver microsphere solution, the silver microsphere surface of the silver microsphere solution having nano-silver particles and a nano-pore structure; preparing a chip with a microfluidic channel provided with a Raman detection area used for being filled with a silver microsphere solution and also serving as an enrichment area and a detection area of a to-be-detected sample; and injecting the silver microsphere solution into a Raman detection area of the microfluidic channel chip, carrying out natural deposition to obtain a monolithic column, and replacing a molecular sieve with silver microspheres as a filler of the monolithic column to obtain the integrated detection chip with collection and detection functions. The chip prepared in the invention realizes rapid quantitative detection of organic components like environmental gas, breathing gas and pesticide residues, has the advantages of environmental protection, simple operation, low cost, high sensitivity and high flux, and has a good application prospect in the fields like environmental monitoring, pesticide residue monitoring and biomedicine.

Description

technical field [0001] The present invention relates to the cross-application fields of material science, micro-nano processing, food safety, environment and biomedicine, and specifically relates to a preparation method and application of a microfluidic chip based on a monolithic column of silver microspheres. Background technique [0002] Food safety, air pollution, and early prevention and screening of major diseases are closely related to the quality of life and health of modern people, and are also major issues that endanger life safety. A large number of recent studies have shown that the increase in PM2.5 in smog is one of the important reasons for the increasing incidence of lung cancer year by year. The atmosphere contains a large amount of nitrogen-containing organic particles derived from automobile exhaust, chemical waste gas, oil fume, etc. Under the irradiation of strong sunlight and ultraviolet rays, an irritating organic compound will be produced, which is har...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65B01L3/00
CPCG01N21/658B01L3/502707
Inventor 沈广霞贺婕李红霞丁显廷
Owner SHANGHAI JIAO TONG UNIV
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