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Trichoderma fermentation medium for antagonizing tea colletotrichum bacteria and fermentation method

A technology of fermentation medium and anthracnose bacteria, which is applied to the fermentation medium and fermentation field of Trichoderma antagonizing tea anthracnose bacteria, and can solve the problems of low inhibition efficiency and high input cost of Trichoderma

Pending Publication Date: 2020-12-22
贵州省生物技术研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problem that the existing Trichoderma is used as a preparation for preventing and treating anthracnose, its inhibition efficiency is relatively low, and the input cost of Trichoderma is high. The purpose of this invention is to provide a Trichoderma fermentation medium and The fermentation method can increase the content of substances that inhibit the growth of tea anthracnose bacteria in the Trichoderma fermentation broth, save costs to a certain extent, and improve fermentation efficiency

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  • Trichoderma fermentation medium for antagonizing tea colletotrichum bacteria and fermentation method
  • Trichoderma fermentation medium for antagonizing tea colletotrichum bacteria and fermentation method
  • Trichoderma fermentation medium for antagonizing tea colletotrichum bacteria and fermentation method

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Experimental program
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Embodiment 1

[0033] Embodiment 1: a kind of Trichoderma fermentation culture medium that antagonizes tea anthracnose pathogen, this substratum is applied to have the Trichoderma fermentation culture that antagonizes tea anthracnose pathogen, fermentation medium is liquid fermentation medium, is made up of following composition: potato, glucose , yeast extract, dipotassium hydrogen phosphate, ferrous sulfate.

[0034] Preferably, the fermentation medium is composed of the following components: potato 200g / L, glucose 15-20g / L, yeast extract 1-2g / L, dipotassium hydrogen phosphate 0.8-1.2g / L, ferrous sulfate 0.5 -0.7g / L, the balance is water.

[0035] Preferably, the fermentation medium is composed of the following components: potato 200g / L, glucose 18-19g / L, yeast extract 1.5-2g / L, dipotassium hydrogen phosphate 0.8-1.0g / L, ferrous sulfate 0.6 -0.7g / L, the balance is water.

[0036] Preferably, the fermentation medium is composed of the following components: potato 200g / L, glucose 18.34g / L,...

Embodiment 2

[0037] Embodiment 2: a kind of trichoderma fermentation method that antagonizes tea anthracnose bacteria may further comprise the steps:

[0038] S101. Plate activation: Inoculate Trichoderma cake with a diameter of 5mm on PDA medium, culture at a constant temperature of 28°C for 7 days; PDA medium configuration: 200g potato, 20g glucose, 20g agar, 1000ml distilled water.

[0039]S102. Seed solution culture: put 100mL PDB medium in a 500mL Erlenmeyer flask, pick a colony from the plate and inoculate it in a shaker flask, culture at 28°C, shaker speed 120r / min, inoculate and ferment after 3 days of culture; PDB culture Base: potato 200g, glucose 20g, distilled water 1000m.

[0040] S103. Fermentation medium preparation: The fermentation medium according to any one of the first aspect is prepared as a liquid fermentation medium, and the prepared liquid fermentation medium is sterilized at 115° C. for 30 minutes before use.

[0041] S104. Fermentation growth and sporulation: put...

Embodiment 3

[0042] Embodiment 3: single factor experiment

[0043] 1. Screening and concentration determination of carbon source in Trichoderma fermentation broth

[0044] (1) Effects of different carbon sources on the mycelial growth and sporulation of Trichoderma

[0045] The basic medium was PDA medium, and 20 g / L glucose, maltose, sucrose, fructose, and lactose were added to replace the carbon source in the PDA medium, and no sugar was set as a control. Trichoderma cakes with a diameter of 5 mm were inoculated in the prepared center (three biological repetitions were set for each experiment) and cultured at a constant temperature of 28°C. After 48 hours, use the "ten" cross method to measure the growth diameter of the colony as an indicator of mycelial growth status. After 7 days of cultivation, use 10 mL of sterilized ddH2O to elute the colony spores to make a spore suspension, and use a hemocytometer to count the spore production. The formula is as follows:

[0046] Total number o...

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Abstract

The invention discloses a trichoderma fermentation medium for antagonizing tea colletotrichum bacteria and a fermentation method, and relates to the technical field of microbial fermentation. According to key points of the technical scheme, the diameter of a tea colletotrichum bacterial colony treated by trichoderma fermentation liquor prepared by the fermentation medium and the fermentation method designed by the invention is 2.98-6.50 cm; the diameter of the tea colletotrichum bacterial colony is generally lower than the diameter 7.0 cm of a tea colletotrichum bacterial colony treated by trichoderma fermentation liquor prepared by an existing trichoderma culture medium, wherein the optimal fermentation medium is prepared from 200 g / L of potatoes, 18.34 g / L of glucose, 1.88 g / L of yeast extract, 0.86 g / L of dipotassium hydrogen phosphate and 0.63 g / L of ferrous sulfate. Under the condition, the diameter of the tea colletotrichum bacterial colony treated by the trichoderma fermentationliquor is 3.01 cm and is reduced by 13% compared with the diameter of the bacterial colony before optimization. A liquid fermentation medium is designed for trichoderma with an effect of antagonizingthe tea colletotrichum bacteria, so that the content of substances for inhibiting the growth of the tea colletotrichum bacteria in the trichoderma fermentation liquor is increased, the cost is savedto a certain extent, and the fermentation efficiency is improved.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, more specifically, it relates to a Trichoderma fermentation medium and a fermentation method for antagonizing tea anthracnose bacteria. Background technique [0002] Tea anthracnose is a common leaf disease caused by fungi on tea trees. It occurs in various tea regions in my country, and usually occurs in Guizhou, Zhejiang, Fujian and other provinces with a lot of rain and high humidity. The disease is mainly caused by anthrax bacteria (Collectotrichums spp.). Most of the germs invade from the young leaves, and cause disease on the mature leaves of the tea tree, causing the leaves to scorch and fall, affecting the photosynthesis of the tea tree, resulting in a reduction in tea production. At present, the prevention and treatment of anthracnose mainly uses disease-resistant varieties, but when the disease occurs seriously, traditional chemical pesticide control methods are still u...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00A01N63/38A01P3/00C12R1/885
CPCC12N1/14C12N3/00A01N63/38Y02A50/30
Inventor 周罗娜周玉锋陈银翠赵兴丽刘辉罗林丽贺圣凌
Owner 贵州省生物技术研究所
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