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Method for extracting yeast polypeptide by using deep eutectic solvent

A technology of deep eutectic solvent and yeast polypeptide, which is applied in the field of food processing, can solve the problems of three wastes discharge, inconvenient operation, and low efficiency, and achieve the effects of high-efficiency extraction, mild extraction conditions, and simple preparation and operation methods

Inactive Publication Date: 2020-12-25
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problems to be solved by the present invention are: the existing methods for extracting yeast active polypeptides are inefficient, inconvenient to operate, and prone to discharge of three wastes, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0031] The preparation method of the above-mentioned deep eutectic solvent comprises: mixing the hydrogen bond acceptor, hydrogen bond donor and deionized water according to the molar ratio of 1:(1~3):(20~80), stirring at 80°C until Clarity yielded a deep eutectic solvent.

[0032] A method for extracting yeast polypeptides using a deep eutectic solvent, the steps are as follows:

[0033] 1. Mix the yeast raw material and deep eutectic solvent according to (1~5)g: (10~50)mL, ultrasonicate at 200~1000W for 20~120min, extract in a constant temperature shaker at 80℃, 150r / min for 4h , through centrifugation, to obtain a yeast polypeptide extract;

[0034] 2. Add ethanol by volume to the yeast polypeptide extract, the volume ratio of yeast polypeptide extract to ethanol is 1: (1-4) ethanol, after mixing, standing, and centrifuging, take the precipitate and wash , centrifuging, and freeze-drying to obtain the yeast polypeptide. Reducing the amount of deep eutectic solvent not on...

Embodiment 1

[0036] A method utilizing a deep eutectic solvent to extract polypeptides in Candida utilis:

[0037](1) Mix choline chloride, oxalic acid and deionized water in a molar ratio of 1:1:20, stir at 80°C until clear, and obtain a deep eutectic solvent;

[0038] (2) Weigh 1.00 g of Candida utilis slime and place it in a beaker, add 10 mL of deep eutectic solvent to it, extract under ultrasonic conditions with a power of 200 W for 100 min, and place it in a constant temperature shaker at 80 ° C and 150 r / min After extracting for 4 hours, the extract was centrifuged at 8000 rpm for 10 minutes to obtain yeast precipitate and yeast peptide extract.

[0039] Use the biuret method to determine the content of polypeptides in the yeast peptide extract, and the formula for calculating the extraction rate of polypeptides is as follows:

[0040] Peptide extraction rate (%) = sample (TCA) polypeptide concentration × sample solution volume / protein content in raw materials × 100

[0041] (No...

Embodiment 2

[0044] A method utilizing a deep eutectic solvent to extract polypeptides in Candida utilis:

[0045] (1) Mix choline chloride, oxalic acid and deionized water in a molar ratio of 1:2:20, stir at 80°C until clear, and obtain a deep eutectic solvent;

[0046] (2) Weigh 2.00 g of Candida utilis slime and place it in a beaker, add 30 mL of deep eutectic solvent to it, extract under ultrasonic conditions with a power of 400 W for 80 min, and place it on a constant temperature shaker at 80 ° C and 150 r / min 4h, centrifuge the extract at 8000rpm for 10min to obtain yeast precipitate and yeast peptide extract.

[0047] Use the biuret method to determine the content of polypeptides in the yeast peptide extract, and the formula for calculating the extraction rate of polypeptides is as follows:

[0048] Peptide extraction rate (%) = sample (TCA) polypeptide concentration × sample solution volume / protein content in raw materials × 100

[0049] (Note: The peptide extraction rate is th...

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PUM

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Abstract

The invention discloses a method for extracting yeast polypeptide by using a deep eutectic solvent. The method is characterized by comprising the following steps of mixing a yeast raw material with the deep eutectic solvent, and carrying out ultrasonic treatment; then, extracting the mixture in a constant-temperature shaking table, and performing centrifuging to obtain a yeast polypeptide leachingsolution; adding a solvent into the yeast polypeptide leaching solution, performing uniformly mixing, standing, centrifuging, taking precipitate, washing, centrifuging and freeze-drying to obtain theyeast polypeptide, wherein the deep eutectic solvent comprises a hydrogen bond donor, a hydrogen bond receptor and deionized water, the hydrogen bond donor is oxalic acid, and the hydrogen bond receptor is choline chloride. The polypeptide is extracted by a solvent method, and the solvent synthesis method comprises the following steps that choline chloride and oxalic acid are heated and bonded together by hydrogen bonds to form the deep eutectic solvent, and the deep eutectic solvent is used as a solvent for extracting polypeptide protein in candida utilis, so that the polypeptide can be wellextracted from the candida utilis, and the obtained product has relatively high purity.

Description

technical field [0001] The invention relates to a preparation method of yeast polypeptide, which belongs to the technical field of food processing. Background technique [0002] Candida utilis, as a safe microorganism, has been certified by the U.S. Food and Drug Administration (FDA) as a yeast that can be used as a food additive, and is also a fungal strain that can be used in health food as stipulated by the Ministry of Health of my country. The cell protein of Candida utilis accounts for about 32% to 75% of the dry matter of the cell, and its content varies greatly due to different strains and culture conditions. The cell wall of Candida utilis accounts for about 20% of the whole weight, and the main components are 57% of β-glucan in the inner wall, 6.6% of mannan oligosaccharide in the outer wall, and 22% of glycoprotein. Among them, the main physiological function of β-glucan is to maintain the structure of the cell wall and maintain the normal physiological shape of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
CPCC07K1/145
Inventor 马霞王灵灵何艳
Owner SHANGHAI INST OF TECH