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A strain of Trichoderma pseudocorning and its application in the control of litchi anthracnose

A technology for Trichoderma pseudocorningi and litchi anthracnose, applied in the fields of application, fungicides, fungi, etc., can solve the problems of pesticide residue environment, increase of drug resistance of pathogenic bacteria, even drug resistance, pollution, etc., and achieve wide adaptability and good Development and application prospects, the effect of little impact on the ecological environment

Active Publication Date: 2022-03-22
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the prevention and treatment of litchi anthracnose, chemical control is the main method. Although chemical control can achieve a certain effect in a short period of time, the continuous use of chemical agents will easily increase the drug resistance or even drug resistance of pathogenic bacteria. At the same time, it is accompanied by problems such as pesticide residues and environmental pollution.

Method used

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  • A strain of Trichoderma pseudocorning and its application in the control of litchi anthracnose
  • A strain of Trichoderma pseudocorning and its application in the control of litchi anthracnose
  • A strain of Trichoderma pseudocorning and its application in the control of litchi anthracnose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1: Isolation, purification and preservation of Trichoderma pseudokoningeni

[0026] (1) Preparation of PDA medium: first wash and peel the potatoes, then weigh 200g of potatoes, chop them into a pot, add 800mL of water to boil for 0.5h, filter with eight layers of gauze, then add 20g of glucose, add water Dilute to 1000mL, stir and mix with a glass rod, add 15g of agar, fully heat and dissolve, then dispense into 250mL Erlenmeyer flasks, sterilize with damp heat at 121°C for 20min, and store for later use.

[0027] (2) Trichoderma strain samples were isolated from the green mold layer on the litchi litchi leaves in the Litchi Base of the College of Horticulture, South China Agricultural University. The strains were isolated and purified with reference to the method of Fang Zhongda et al., and slightly modified: use inoculation Pick a little green mold layer on the PDA medium plate with a needle, place it in an incubator at 28°C and cultivate it. After the gre...

Embodiment 2

[0032] Embodiment 2: confrontation culture method measures the activity of Trichoderma pseudokoningeni

[0033] (1) The preparation of PDA medium is the same as in Example 1.

[0034] (2) Preparation of single colony and bacterial plate of Trichoderma pseudocornifolia: pick Trichoderma pseudocorniensis hyphae on a PDA medium plate with a sterilized toothpick, and culture at 28°C for 3-4 days to obtain a single colony; The edge of Trichoderma pseudoconingi colony is punched to obtain a round fungus disc.

[0035] (3) Cultivation of litchi anthracnose bacteria and preparation of bacteria plates: inoculate the litchi anthrax strains stored at -20°C on a PDA medium plate for activation, and punch them with a puncher with a diameter of 5 mm after the colonies cover the plate Take the edge of the colony to obtain a round disk.

[0036] (4) Activity measurement: on the PDA plate culture medium of 9cm, insert the Trichoderma pseudocornibacterium dish that step (2) prepares and the l...

Embodiment 3

[0038] Embodiment 3: growth rate method measures the activity of Trichoderma pseudokoningenii biological preparation

[0039] (1) The preparation of PDA medium is the same as Example 1; the preparation of PDB liquid medium is except adding agar, and other is identical with the formula of PDA solid medium, and after the medicament weighed is fully dissolved, be subpackaged in conical flasks (each Fill the bottle with 100mL culture solution), stopper, bandage, sterilize with damp heat at 121°C for 20min, cool and store for later use.

[0040] (2) Preparation of Trichoderma pseudocorningii aseptic fermentation filtrate (biological agent): take the Trichoderma pseudocorningii bacteria plate prepared in step (2) of Example 2 and insert into step (1) to prepare the PDB nutrient solution equipped with 100mL Place in a Erlenmeyer flask at 28°C with a shaker speed of 180rpm and shake in the dark for 5 days, then centrifuge at 5000rpm for 10min, absorb the supernatant, and filter out th...

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Abstract

The invention belongs to the technical field of biological control of plant diseases, and discloses a strain of Trichoderma pseudocorningum and its application in preventing and treating litchi anthracnose. The name of the strain is Trichoderma koningiopsis TJ5, and it was preserved in the Guangdong Microbial Culture Collection Center, referred to as GDMCC, on August 8, 2020. Address: 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou, Guangdong Provincial Institute of Microbiology, the preservation number is GDMCC No:61129. The bacterial strain of the present invention is an antagonistic bacteria screened from litchi. Because it coexists with litchi for a long time and coexists mutually, it forms a series of survival mechanisms similar to it. It is used to prevent and treat litchi diseases. For the edible and processed products of litchi It is safer and more reliable; the bacterial strain has excellent inhibitory effect on litchi anthracnose bacteria, has strong antibacterial ability, and has important practical significance for the prevention and treatment of litchi anthracnose.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a strain of Trichoderma pseudocorningum and its application in preventing and treating litchi anthracnose. Background technique [0002] Litchi (Litchi chinensis Sonn.) is a subtropical perennial evergreen plant and an important subtropical economic crop, belonging to Sapindaceae. my country's litchi was originally produced in the southern region, including Guangdong, Guangxi, Fujian, Hainan and other provinces. Litchi, as a good product among fruits, has juicy pulp, strong fragrance, tender and refreshing taste. It has long been known as "China's precious fruit" and has extremely high commercial value. However, since litchi fruit ripens mostly in high temperature and high humidity seasons, the fruit after harvesting is prone to browning and rot and deterioration, and litchi anthracnose is the main cause of litchi fruit rot and deteriorat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00C12R1/885
CPCC12N1/14A01N63/38C12R2001/885C12N1/145
Inventor 习平根朱洪辉袁玉花李敏慧孔广辉司徒俊键杨文晟姜子德
Owner SOUTH CHINA AGRI UNIV
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