Acid-resistant salt-tolerant microbial agent for degrading kitchen garbage and preparation method thereof

A microbial agent and kitchen waste technology, applied in the field of microorganisms, to achieve excellent acid resistance and salt resistance

Inactive Publication Date: 2021-01-01
深圳市嘉佳环保技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, kitchen waste in my country has the characteristics of high salinity, high organic matter content and high water content. Among them, excessively high salt content will inhibit microorganisms from performing their fermentation functions, and organic small molecular acids (such as lactic acid and propionic acid) produced during the fermentation process , acetic acid, etc.) will also inhibit the fermentation function of microorganisms to a certain extent

Method used

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  • Acid-resistant salt-tolerant microbial agent for degrading kitchen garbage and preparation method thereof
  • Acid-resistant salt-tolerant microbial agent for degrading kitchen garbage and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation method of the acid-resistant and salt-resistant microbial bacterial agent for degrading kitchen waste provided by this embodiment includes the following steps:

[0025] (1) Bacillus thuringiensis bacterial classification, Bacillus coagulans bacterial classification, Bacillus amyloliquefaciens bacterial classification, Bacillus subtilis bacterial classification are respectively inoculated into the shaking flask that is equipped with the activation medium of 25% by volume ratio, respectively at temperature Cultivate for 18 hours at 35-40°C and shaker flask rotation speed 180r / min. The activated medium is beef extract peptone liquid medium; the activated Bacillus thuringiensis strains, Bacillus coagulans strains, Bacteria and Bacillus subtilis were subjected to two gradient cultures respectively. The conditions for each gradient culture were: temperature 28-32°C, stirring speed 150r / min, culture time 12h, and the pH value of the first gradient medium was 5.5...

Embodiment 2

[0029] Repeat step (1) to step (2) of Example 1, take the following raw materials by weight: 7 parts of Bacillus thuringiensis bacterium liquid, 9 parts of Bacillus coagulans bacterium liquid, 2 parts of Bacillus amyloliquefaciens bacterium liquid part, 8 parts of Bacillus subtilis bacterium liquid, 0.5 part of saccharomyces bacterium liquid, 0.5 part of Aspergillus oryzae bacterium liquid; The yeast liquid and the Aspergillus oryzae liquid are inoculated in the fermentation medium, and fermented and cultivated for 36 hours at a temperature of 25-35°C and a stirring speed of 240r / min to obtain an acid-resistant and salt-tolerant microbial bacterial agent, wherein the fermentation culture The base is: molasses 4%, corn steep liquor powder 8%, sodium chloride 4%, water balance, and the pH value is 4.

Embodiment 3

[0031]Repeat step (1) to step (2) of Example 1, and take the following raw materials in parts by weight: 9 parts of Bacillus thuringiensis bacterium liquid, 8 parts of Bacillus coagulans bacterium liquid, 4 parts of Bacillus amyloliquefaciens bacterium liquid Parts, 6 parts of Bacillus subtilis bacterial liquid, 1 part of saccharomyces bacterial liquid, 1 part of Aspergillus oryzae bacterial liquid; The yeast liquid and the Aspergillus oryzae liquid are inoculated in the fermentation medium, and fermented and cultivated for 36 hours at a temperature of 25-35°C and a stirring speed of 240r / min to obtain an acid-resistant and salt-tolerant microbial bacterial agent, wherein the fermentation culture The base is: molasses 4%, corn steep liquor powder 8%, sodium chloride 4%, water balance, and the pH value is 4.

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Abstract

The invention discloses an acid-resistant salt-tolerant microbial agent for degrading kitchen garbage and a preparation method thereof. The acid-resistant salt-resistant microbial agent is prepared from the following raw materials in parts by weight of 4 to 10 parts of bacillus thuringiensis bacterial liquid, 4 to 10 parts of bacillus coagulans bacterial liquid, 1 to 5 parts of bacillus amyloliquefaciens bacterial liquid, 5 to 10 parts of bacillus subtilis bacterial liquid, 0.5 to 2 parts of saccharomycetes bacterial liquid and 0.5 to 2 parts of aspergillus oryzae bacterial liquid. The bacillus thuringiensis bacterial liquid, the bacillus coagulans bacterial liquid, the bacillus amyloliquefaciens bacterial liquid, the bacillus subtilis bacterial liquid, the saccharomycetes bacterial liquidand the aspergillus oryzae bacterial liquid are mixed and fermented to prepare the microbial agent, and the microbial agent can effectively degrade cellulose, protein, starch, grease and other components in the kitchen garbage; and the kitchen garbage is sufficiently fermented, and the acid-resistant salt-resistant microbial agent has excellent acid resistance and salt resistance, and can normally exert the functions under high salt content and acidic conditions.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to an acid-resistant and salt-resistant microbial bacterial agent for degrading kitchen waste and a preparation method thereof. Background technique [0002] Kitchen waste refers to the waste generated in the daily life of residents and activities such as food processing, catering services, and unit meals, including discarded vegetable leaves, leftovers, leftovers, fruit peels, eggshells, tea dregs, bones, etc. The main sources are home kitchens, restaurants, restaurants, canteens, markets and other industries related to food processing. [0003] At present, the treatment methods of kitchen waste in my country are mainly as follows: (1) fermented to make organic fertilizer or soil conditioner; (2) directly used as animal feed after recycling; (3) oil separation and oil recovery; (4) ) incineration or landfill; Among them, the treatment method of using microorganisms to fermen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14B09B3/00C12R1/07C12R1/125C12R1/69
CPCC12N1/20C12N1/16C12N1/14B09B3/00
Inventor 黄河唐勇
Owner 深圳市嘉佳环保技术有限公司
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