Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of indigestible branched glucan and its processing method

A processing method and indigestible technology, which are applied in the field of indigestible branched glucan and its processing, can solve the problems that the quality and quality cannot meet the individual needs of consumers, the production cost is high, the research start is late, and the like, and social benefits are achieved. and significant economic benefits, improving people's health, and increasing the value of agricultural and sideline products

Active Publication Date: 2022-07-05
JIANGNAN UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

European and American multinational companies such as Ryanair, Cargill, and Roquette have obtained new products such as resistant starch and resistant dextrin through chemical and physical modification, and have formed large-scale production and sales; Research started late, most of them are in the stage of laboratory research, only a few companies have started pilot production, but there is a big gap in product performance compared with foreign countries
The existing commercial production methods of branched glucan or resistant starch mainly include acid-heat pyrodextrinization reaction and starch sugar chain temperature-controlled crystallization method, but these methods have complex reaction process, low preparation efficiency, poor stability, Bottleneck problems such as poor nutritional quality and high production costs
Therefore, solving the problem that the quality of existing products is difficult to meet the national health industry strategy and the individual needs of consumers has become the top priority in the field of starch nutrition products.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of indigestible branched glucan and its processing method
  • A kind of indigestible branched glucan and its processing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] For the acquisition process of α-glucosidase, see the literature Microbial Starch-Converting Enzymes: Recent Insights and Perspectives, Comprehensive Reviews in Food Science and Food Safety, 2018, 17(5): 1238-1260, including enzyme gene mining, engineering bacteria construction, Fermentation and production of enzymes.

[0031] Specifically, the α-glucosidase gene derived from microorganisms such as Aspergillus niger, Acremonium, Paecilomyces or Paenibacillus is connected to the expression vector pET-15b(+) to obtain a recombinant plasmid. The recombinant plasmid was transformed into E.coil competent cells by chemical transformation, and 1 mL of LB medium was added, and cultured in a shaker at 37ºC, 200 r / min for 1-1.5 h; 100-200 μL of the above bacterial solution was taken , spread on LB plates containing ampicillin (Amp), and then cultured in a 37ºC incubator for 12 h. Successfully transformed single colonies were picked on LB plates and placed in LB liquid medium con...

Embodiment 2

[0033] Take by weighing 10g corn starch and be made into 10% starch milk with mass percent concentration, be placed in a boiling water bath and heated to complete gelatinization; Treat that the temperature is reduced to 60 ° C, add the α-amylase of 200 U / g starch and be incubated for 0.5 h, Subsequently, 100 U / g starch α-glucosidase was added to continue the reaction for 1 h; the enzyme was inactivated by heating, centrifuged, and the obtained supernatant was dried to obtain a branched glucan product. In this process, the DE value of α-amylase hydrolyzed starch chain was 7.3.

[0034] After analysis and determination, it can be seen that the average molecular weight of the prepared branched glucan is 4800 g / mol, the content of α-1,3 bonds is 8.2%, the content of α-1,6 bonds is 49%, and the proportion of indigestible nutrient fragments reaches 80%. %. figure 1 and figure 2 They are the structural schematic diagram and NMR chart of the prepared indigestible branched glucan, r...

Embodiment 3

[0036] Take by weighing 40g wheat starch and be made into 40% starch milk with mass percent concentration, be placed in boiling water bath and be heated to complete gelatinization; Treat that temperature is down to 30 DEG C, add the α-amylase of 100 U / g starch and be incubated for 1 h, Subsequently, 20 U / g starch α-glucosidase was added to continue the reaction for 12 hours; the enzyme was inactivated by heating, centrifuged, and the obtained supernatant was dried to obtain a branched glucan product. In this process, the DE value of α-amylase hydrolyzed starch chain was 5.5.

[0037] After analysis and determination, it can be seen that the average molecular weight of the prepared branched glucan is 6100 g / mol, the content of α-1,3 bonds is 7.5%, the content of α-1,6 bonds is 60%, and the proportion of indigestible nutrient fragments is 83%. .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an indigestible branched glucan and a processing method thereof, belonging to the technical field of health food processing. The invention includes: weighing a certain quality of starch to prepare starch milk with a concentration of 10-40wt%, placing it in a boiling water bath and heating to complete gelatinization; when the temperature drops to 30-60°C, adding 20-200U / g 淀粉 α-amylase and insulation reaction 0.1-3h, then add 20-200U / g 淀粉 The α-glucosidase was continuously reacted for 1-12 h; the enzyme was inactivated, the solid-liquid was separated, and the obtained supernatant was dried to obtain the indigestible branched glucan. The method of the invention not only has green technology, simple operation procedure and low production cost, but also has good probiotic physiological activity, and can be used as a new type of dietary fiber in many fields such as health food, beverage and medicine.

Description

technical field [0001] The invention relates to an indigestible branched glucan and a processing method thereof, belonging to the technical field of health food processing. Background technique [0002] Starch is widely distributed in the plant kingdom. It is the main energy storage material for seeds or fruits of food crops, and it is also the main raw material for modern food industry. As a typical component of the daily rice and noodles staple food of Chinese residents, starch is also the main source of energy intake by the body, accounting for 55%-70% of the total energy intake. Natural cereal starch is generally considered to be low-glycemic starch, but it is prone to gelatinization after thermal processing of food and almost completely converted into digestible starch, which is a high-glycemic food that releases energy quickly. European and American multinational companies such as Ruian, Cargill, Roquette, etc. have obtained new products such as resistant starch and r...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14C12P19/08A23L33/125A23L2/60
CPCC12P19/14C12P19/08A23L33/125A23L2/60A23V2002/00A23V2250/5034
Inventor 缪铭胡秀婷杨玉琪陈一枚孙纯锐邱立忠金征宇
Owner JIANGNAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com