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Application of plant lactobacillus to preservation of bacillus subtilis solid inoculant and method for extending preservation period of bacillus subtilis

A technology of Bacillus subtilis and plant lactic acid bacteria, which is applied in the field of microorganisms, can solve the problems such as the decrease of the number of viable bacteria of Bacillus subtilis, and achieve the effects of prolonging the preservation period and inhibiting the decrease of the number of viable bacteria.

Active Publication Date: 2021-01-05
COFCO BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the process of preservation, the number of viable Bacillus subtilis still has a certain degree of decline, because it is imperative to develop a method that can slowly reduce the number of viable Bacillus subtilis under the condition of long-term preservation

Method used

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  • Application of plant lactobacillus to preservation of bacillus subtilis solid inoculant and method for extending preservation period of bacillus subtilis

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0053] This preparation is used to illustrate the activation of Bacillus subtilis seeds

[0054] The Bacillus subtilis stored in the ultra-low temperature refrigerator was naturally thawed 0.5h in advance. Thereafter, 0.1% by volume of the inoculum was inoculated into a sterilized seed medium containing 0.5% by weight of yeast powder, 1% by weight of peptone, 0.5% by weight of glucose, and 1% by weight of sodium chloride for seed activation. The culture condition is 30±2°C, the rotation speed is 100rppm, when the OD value grows to 2.6, the pH is about 6.0, the first-level seed shake flask is finished, and the number of viable bacteria is 10 9 cfu / ml.

preparation example 2

[0056] This preparation example is used to illustrate the activation of plant lactic acid bacteria seeds

[0057] The Lactobacillus plantarum stored in an ultra-low temperature refrigerator was naturally thawed 0.5h in advance. Thereafter, the seeds were inoculated into sterilized MRS broth medium at an inoculum size of 0.2% by volume to perform activation culture of the seeds. Incubate at 35±2°C. When the OD value grows to 6-7, the pH is about 4.0, the first-level seed shake flask is over, and the number of viable bacteria is 10 10 cfu / ml.

Embodiment 1

[0059] This embodiment is used to illustrate the method for prolonging the shelf life of Bacillus subtilis solid microbial agent provided by the present invention

[0060] (1) fermentation culture of Bacillus subtilis

[0061] Medium: 1.8% by weight of soybean meal, 3.4% by weight of corn flour, 0.2% by weight of high-temperature amylase, 0.8% by weight of yeast extract powder, 0.5% by weight of glucose, 0.2% by weight of manganese sulfate, natural pH, sterilized at 121°C for 30 minutes for later use.

[0062] The activated seed solution of Bacillus subtilis prepared in Preparation Example 1 was inoculated in the above-mentioned medium with an inoculation amount of 0.1% by volume, cultivated at 37 ± 2°C, and the rotation speed was 120rppm. After the OD value grew to about 9.5 and the pH was about 7.2, ferment The end of the shake flask, the number of viable bacteria is 10 10 cfu / ml.

[0063] (2) Fermentation and cultivation of Lactobacillus plantarum

[0064] Culture medium...

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Abstract

The invention relates to the field of microorganisms, in particular to application of plant lactobacillus to preservation of a bacillus subtilis solid inoculant and a method for extending the preservation period of bacillus subtilis. The method comprises the following steps: mixing plant lactobacillus thalli with bacillus subtilis thalli to obtain mixed thalli; mixing the mixed thalli with a frozen carrier, and freeze-drying an obtained mixture; the frozen carrier is selected from at least one of soluble starch, anhydrous sodium sulfite, halite and glucose. According to the plant lactobacillusand the method, the bacillus subtilis and the plant lactobacillus are mixed to prepare the solid inoculant, and thus, the decrease of the viable count of the bacillus subtilis in the preservation process can be effectively inhibited, and the preservation period is extended.

Description

technical field [0001] The invention relates to the field of microbes, in particular to the application of plant lactic acid bacteria in the preservation of bacillus subtilis solid microbial agents and a method for prolonging the preservation period of bacillus subtilis. Background technique [0002] Bacillus subtilis is an aerobic spore-forming bacterium that widely exists in nature. Bacillus subtilis has excellent stability in the spore state (exists in the spore state at the mature stage), has the characteristics of oxidation resistance, extrusion resistance, high temperature resistance, stagnant water resistance, acid resistance and bile salt resistance, and can secrete a variety of enzymes Substances and growth unknown factors. Some data show that Bacillus subtilis can withstand high temperature of 60°C for a long time, and Bacillus subtilis also has a high tolerance under storage and processing conditions; Bacillus subtilis has a higher survival rate in the range of p...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12N1/20C12R1/125C12R1/25
CPCC12N1/04C12N1/20
Inventor 佟毅刘利利邴狄祥张琳卢宗梅金渭武郑晓卫
Owner COFCO BIOTECHNOLOGY CO LTD
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