Application of plant lactobacillus to preservation of bacillus subtilis solid inoculant and method for extending preservation period of bacillus subtilis
A technology of Bacillus subtilis and plant lactic acid bacteria, which is applied in the field of microorganisms, can solve the problems such as the decrease of the number of viable bacteria of Bacillus subtilis, and achieve the effects of prolonging the preservation period and inhibiting the decrease of the number of viable bacteria.
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[0052]Preparation Example 1
[0053]This preparation example is used to illustrate the activation of Bacillus subtilis seeds
[0054]The Bacillus subtilis will be naturally thawed 0.5h in advance in the ultra-low temperature refrigerator. Then, an inoculum of 0.1% by volume was inoculated into a sterilized seed medium containing 0.5% by weight of yeast powder, 1% by weight of peptone, 0.5% by weight of glucose, and 1% by weight of sodium chloride to activate the seed culture. The culture condition is 30±2℃, the rotation speed is 100rppm, when the OD value reaches 2.6, the pH is about 6.0, the first-level seed shake flask ends, and the number of viable bacteria is 109cfu / ml.
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[0055]Preparation Example 2
[0056]This preparation example is used to illustrate the activation of plant lactic acid bacteria seeds
[0057]The Lactobacillus plantarum is naturally thawed 0.5h in advance in the ultra-low temperature refrigerator. After that, the seed was inoculated into a sterilized MRS broth medium with an inoculum amount of 0.2% by volume to activate the seeds. Cultivate at 35±2℃. When the OD value grows to 6-7, the pH is about 4.0, the first-level seed shake flask ends, and the number of viable bacteria is 1010cfu / ml.
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[0058]Example 1
[0059]This example is used to illustrate the method for extending the shelf life of Bacillus subtilis solid inoculum provided by the present invention
[0060](1) Fermentation culture of Bacillus subtilis
[0061]Culture medium: soybean meal 1.8% by weight, corn meal 3.4% by weight, high temperature amylase 0.2% by weight, yeast extract powder 0.8% by weight, glucose 0.5% by weight, manganese sulfate 0.2% by weight, natural pH, and sterilized at 121°C for 30 minutes for use.
[0062]The activated seed solution of Bacillus subtilis prepared in Preparation Example 1 was inoculated into the above medium with an inoculum amount of 0.1% by volume, cultured at 37±2°C at a rotation speed of 120rppm, and when the OD value reached about 9.5 and the pH was about 7.2, fermentation After shaking the flask, the number of viable bacteria is 1010cfu / ml.
[0063](2) Fermentation culture of Lactobacillus plantarum
[0064]Medium: yeast extract powder 3% by weight, sodium acetate 0.5% by weight, tria...
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