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Calophyllum inophyllum aqueous extract powder as well as preparation, immunity enhancing effect and application thereof

A technology of water extract and crabapple fruit, applied in the preparation of immune-enhancing drugs, in the field of preparation of fresh crabapple fruit water extract powder, can solve the problem of ignorance of biological activity

Inactive Publication Date: 2021-01-15
北京恒润泰生医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the biological activity of a single component of fresh water extract powder has been reported, the combined biological activity of all components is unknown

Method used

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  • Calophyllum inophyllum aqueous extract powder as well as preparation, immunity enhancing effect and application thereof
  • Calophyllum inophyllum aqueous extract powder as well as preparation, immunity enhancing effect and application thereof
  • Calophyllum inophyllum aqueous extract powder as well as preparation, immunity enhancing effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 fresh crabapple water extract powder

[0037] Take fresh crabapple fruit, wash it with tap water, take 100g of pulp and chop it, heat it to boiling in 150mL of distilled water for 4h, stir (220rpm / min) until the pulp becomes puree, cool to room temperature, filter, the filtrate is viscous, and the filter cake Wash twice with distilled water, each 150mL distillation, then discard the filter cake, combine the collected filtrate, and concentrate under reduced pressure to obtain 6 g of deep red powder with good resolubility, which is fresh crabapple water extract powder.

Embodiment 2

[0038] Embodiment 2 measures the chromatogram and mass spectrometry ion flow spectrum of fresh crabapple water extract powder

[0039] 2-1. Preparation of sample solution (10mg / mL)

[0040] Weigh 26.7mg fresh crabapple water extract powder and dissolve the powder in 2.67mL ultrapure water. The obtained solution was ultrasonically shaken for 1 minute, and then centrifuged at 13000r / min for 10 minutes. Take the supernatant, pass through a 0.22μm filter membrane, and place it in a sample bottle for chromatography and mass spectrometry.

[0041] 2-2. Chromatographic conditions

[0042] Chromatographic conditions

[0043] Column: Waters, Acquity HSS T3 column (2.1×100mm i.d., 1.7μm);

[0044] Injection volume: 2μL;

[0045] Mobile phase: water (0.1% formic acid), acetonitrile;

[0046] Wash the column with the mobile phase according to the gradient in Table 1.

[0047] Table 1 Mobile phase gradient table

[0048]

[0049] 2-3. Measurement chromatogram

[0050] Measur...

Embodiment 3

[0053] Embodiment 3 specifies the structure of 25 components in the fresh crabapple water extract powder

[0054] The UPLC chromatography of Example 2 was coupled with mass spectrometry, and the UPLC-mass spectrometry of fresh crabapple water extract powder was determined. The mass spectrometry conditions are two modes of electrospray ionization, positive ion and negative ion. Ion mode parameters: capillary voltage is 1000V, desolvation gas flow rate is 800L / h, temperature is 450°C, source temperature is 120°C, cone gas flow rate is 50L / h, spray pressure is 6bar, fragmentation voltage is 20-45V, The sampling cone voltage is 6V, the acquisition mode is MSE continuum resolution mode, the data acquisition range of the mass number to charge number ratio (m / z) of charged particles is 100-1500, the Trap fragmentation voltage of the low energy channel is 6V, and the high energy The channel Trap fragmentation voltage selection gradient voltage is 20-60V, and LE (leucine enkephalin) i...

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Abstract

The invention discloses fresh calophyllum inophyllum aqueous extract powder, a preparation method of the fresh calophyllum inophyllum aqueous extract powder and an application of the fresh calophylluminophyllum aqueous extract powder in enhancing immunity. The fresh calophyllum inophyllum aqueous extract powder prepared by the preparation method disclosed by the invention contains 18 quinic acidcompounds and 7 quercetin compounds. Experiments prove that the fresh calophyllum inophyllum aqueous extract powder has a good immunity enhancing effect. Therefore, the invention provides an effectivetechnical means for immunological enhancement treatment.

Description

technical field [0001] The invention relates to a method for preparing water extract powder of fresh crabapple fruit, and relates to the application of the water extract powder of fresh crabapple fruit prepared by the method in preparing immune-enhancing medicine. The invention belongs to the field of biomedicine. Background technique [0002] Immunocompromise can have various consequences, such as the induction of infectious diseases (various influenzas) and skin diseases (condyloma acuminatum, flat warts, psoriasis, genital herpes, allergic purpura and chronic idiopathic thrombocytopenic purpura ). Patients with various tumors (lung cancer, liver cancer, ovarian cancer, kidney cancer, breast cancer, cervical cancer, osteosarcoma cancer, digestive system tumors and blood system tumors) are faced with low immune function and are prone to secondary infectious diseases. Although there are a variety of non-specific immune enhancers (BCG, nuclear casein injection, levamisole, ...

Claims

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Application Information

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IPC IPC(8): A61K36/73A61K31/222A61K31/225A61K31/7048A61P7/02A61P9/10A61K131/00
CPCA61K31/222A61K31/225A61K31/7048A61K36/73A61K2236/331A61P7/02A61P9/10
Inventor 彭师奇赵明黄飞
Owner 北京恒润泰生医药科技有限公司
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