Preparation method of ampelopsis grossedentata liquid medicine and method for preventing and treating rana spinosa rotten skin disease
A technology for showing toothed snake grapes and skin rot, which is applied in the fields of medical formula, drug delivery, anti-infective drugs, etc., can solve the problems of farmers' economic losses, water pollution, drug residues, etc., and achieve the effect of removing parasites on the body
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Embodiment 1
[0014] The preparation method of the bacterial suspension of the pathogenic strain of Rana spinosa rot skin disease:
[0015] ①Choose healthy, disease-free, active, and 40-100g adult frogs as experimental materials;
[0016] ②Isolate the strain from the muscle, liver and other organs of the diseased frog suffering from spinosa rotting disease, and then infect the healthy frog with this strain; when the healthy frog has rot skin disease, isolate the strain as The pathogenic strain was finally identified as Aeromonas hydrophila (Aeromonas hydrophila) by biochemical assay and DNA molecular detection.
[0017] ③ Inoculate the pathogenic strains in LB liquid medium and culture them on a shaker at 29-32°C and 150rpm for 24 hours; then centrifuge the bacterial solution at 6000rpm for 10min to collect the strains, and then elute with 0.68% sterile saline to obtain bacteria solution; finally use the McFarland turbidimetric tube as a reference, dilute with sterile saline, and make 3*10...
Embodiment 2
[0028] The purpose of this embodiment is to actually test the effect of the 0.03-0.032g / ml of the snake grape medicinal liquid on the prevention and treatment of spinosa spinosa rot skin disease:
[0029] The first batch of experiments were skin scratch infection experiments, including experimental group, blank group, scratch non-infection group, and infection control group; the second batch of experiments were injection-induced infection experiments, including experimental group and infection control group. For each batch of experiments, healthy spiny frogs with similar size and activity were used as the experimental objects, and the concentration was 3*10 8 The suspension of Aeromonas hydrophila bacteria per ml is the pathogenic bacterium for infection, and the medicinal liquid of 0.03-0.032 g / ml is used as the experimental medicinal liquid.
[0030] First batch of experiments:
[0031] test group:
[0032] ⑴. Experiment site: The whole experiment is carried out in the lab...
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