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Preparation method of ampelopsis grossedentata liquid medicine and method for preventing and treating rana spinosa rotten skin disease

A technology for showing toothed snake grapes and skin rot, which is applied in the fields of medical formula, drug delivery, anti-infective drugs, etc., can solve the problems of farmers' economic losses, water pollution, drug residues, etc., and achieve the effect of removing parasites on the body

Inactive Publication Date: 2021-01-22
HUAIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although there are a large number of artificial breeding from the north to the south of the country, only a few of them have achieved success and produced significant economic benefits. The key factor for complete failure; skin rotting disease is the most common infectious disease in the breeding of spiny-breasted frogs. Its main symptoms are: white spots appear on the snout and back of the head in the early stage of the disease, and then the skin of the spots rots to expose the muscles and spread further If it spreads to the trunk and limbs, it can die in a short period of time. The disease has a long epidemic time, and it occurs from mid-spring to late autumn, especially in June-July. Once it occurs, it can spread rapidly and cause a large number of deaths. heavy economic losses; at present, the prevention and treatment methods of rot skin disease are mainly based on prevention, and the strategy of preventive disinfection combined with isolation medicinal bath is adopted. Preventive disinfection is to regularly disinfect new ponds and breeding ponds. Potassium manganate, povidone-iodine, trichloroisocyanuric acid, etc. are prepared in a certain concentration and splashed in the whole pool; the isolation dipping is to isolate the affected individual or conduct dipping in the original pool, and the drugs used are mainly antibiotics, such as: More than ten kinds of antibiotics such as kanamycin, gentamicin, chloramphenicol, cephalosporin, and ceftazidime, and other antibacterial drugs such as: compound sulfadimethoxine, florfenicol, levofloxacin, etc.; antibiotics and antibacterial drugs The prevention and treatment of rotting skin disease is effective in the short term, but at the same time, it will inevitably cause water pollution and drug residues in commercial frogs. Long-term use will also lead to the emergence of drug resistance of pathogenic bacteria, which runs counter to the concept of pursuing green, environmental protection and safety. Therefore, seeking efficient, safe and non-polluting Chinese herbal medicine control technology has become a new topic for the cultivation of spiny-breasted frogs and other aquaculture

Method used

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  • Preparation method of ampelopsis grossedentata liquid medicine and method for preventing and treating rana spinosa rotten skin disease
  • Preparation method of ampelopsis grossedentata liquid medicine and method for preventing and treating rana spinosa rotten skin disease

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Experimental program
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Effect test

Embodiment 1

[0014] The preparation method of the bacterial suspension of the pathogenic strain of Rana spinosa rot skin disease:

[0015] ①Choose healthy, disease-free, active, and 40-100g adult frogs as experimental materials;

[0016] ②Isolate the strain from the muscle, liver and other organs of the diseased frog suffering from spinosa rotting disease, and then infect the healthy frog with this strain; when the healthy frog has rot skin disease, isolate the strain as The pathogenic strain was finally identified as Aeromonas hydrophila (Aeromonas hydrophila) by biochemical assay and DNA molecular detection.

[0017] ③ Inoculate the pathogenic strains in LB liquid medium and culture them on a shaker at 29-32°C and 150rpm for 24 hours; then centrifuge the bacterial solution at 6000rpm for 10min to collect the strains, and then elute with 0.68% sterile saline to obtain bacteria solution; finally use the McFarland turbidimetric tube as a reference, dilute with sterile saline, and make 3*10...

Embodiment 2

[0028] The purpose of this embodiment is to actually test the effect of the 0.03-0.032g / ml of the snake grape medicinal liquid on the prevention and treatment of spinosa spinosa rot skin disease:

[0029] The first batch of experiments were skin scratch infection experiments, including experimental group, blank group, scratch non-infection group, and infection control group; the second batch of experiments were injection-induced infection experiments, including experimental group and infection control group. For each batch of experiments, healthy spiny frogs with similar size and activity were used as the experimental objects, and the concentration was 3*10 8 The suspension of Aeromonas hydrophila bacteria per ml is the pathogenic bacterium for infection, and the medicinal liquid of 0.03-0.032 g / ml is used as the experimental medicinal liquid.

[0030] First batch of experiments:

[0031] test group:

[0032] ⑴. Experiment site: The whole experiment is carried out in the lab...

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Abstract

The invention provides a preparation method of ampelopsis grossedentata liquid medicine. The preparation method comprises the following steps: collecting ampelopsis grossedentata and extracting liquidmedicine to obtain the ampelopsis grossedentata liquid medicine. The method for preventing and treating the rana spinosa rotten skin disease by using the liquid medicine comprises the following steps: performing medicated bath on adult rana spinosa by using 0.03-0.032 g / ml of the ampelopsis grossedentata liquid medicine once every month for 7-10 h from the beginning of April to the end of autumnwhen the rotten skin disease occurs, so that a rana spinosa body can just submerge the back. When some frogs in an adult frog culture pond have rotten skin disease symptoms, the sick frogs are caughtand independently placed in plastic basins or other containers to be subjected to isolation medicated bath, medicated bath is conducted on the frogs with the liquid medicine concentration of 0.03-0.032 g / ml for 7-10 h once and once every 3 days till frog bodies recover, and meanwhile preventive medicated bath is conducted on the culture pond where the sick frogs occur once every 10 days and 2-3 times according to situations. The ampelopsis grossedentata is used for preventing and treating the rotten skin disease, can efficiently prevent diseases and remove ectoparasites, is non-toxic, pollution-free, residue-free, simple and easy to implement, and is very suitable for cultivation of the rana spinosa.

Description

Technical field: [0001] The invention relates to the technical field of aquaculture, in particular to a method for preparing a medicinal liquid of octopus japonica and a method for preventing and treating rotting skin disease of spiny frog. Background technique: [0002] Spinostrophes, also known as stone frogs and stone sticks, belong to the class Amphibians, Ranaidae, and Rana genus. They are mainly distributed in the Yangtze River Basin, South China, and Southwest China. Because the frogs are delicious, nutritious, and have certain Because of its medicinal value, it has always been a popular game treasure; since the initial success of artificial breeding in Fujian in the early 1980s in my country, after nearly 40 years of development, the breeding scale of spiny-breasted frogs has expanded rapidly, basically covering the Yangtze River The natural distribution area in the basin and its south, including Zhejiang, Jiangxi, Fujian, Anhui, Jiangsu, Hunan, Hubei, Guizhou, Sichuan...

Claims

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Application Information

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IPC IPC(8): A61K36/87A61P31/02A61P33/14
CPCA61K9/0014A61K36/87A61K2236/331A61P31/02A61P33/14
Inventor 刘胜贵向孙军
Owner HUAIHUA UNIV
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