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Large-scale preparation method of marine source type I collagen subunits

A technology of type I and collagen is applied in the field of large-scale preparation of marine source type I collagen subunits, which can solve problems such as inability to realize industrialized production, and achieve the effects of easy process control, clear amino acid sequence, and low production cost.

Active Publication Date: 2021-01-29
THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these preparation methods can only obtain milligram-level and microgram-level collagen subunit samples, and cannot achieve industrial production.

Method used

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  • Large-scale preparation method of marine source type I collagen subunits
  • Large-scale preparation method of marine source type I collagen subunits
  • Large-scale preparation method of marine source type I collagen subunits

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Experimental program
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Embodiment 1

[0034] Such as figure 1 Shown, a kind of large-scale preparation method of marine source type I collagen subunit, comprises the following steps:

[0035] (1) 100kg tilapia fish scales / fish skin / maw are dropped into the cleaning tank, add 1000L of sodium bicarbonate solution, the mass volume concentration of the solution is 3%, stir at room temperature for 5h, after washing with water, add in the reaction kettle, Add 0.5M acetic acid solution and stir for extraction. After the extract is decontaminated by a centrifuge at 10,000 rpm, the obtained supernatant is the type I collagen crude extract solution. Add 3% (W / V) sodium chloride to the type I collagen crude extract solution for salting out, and centrifuge with a centrifuge at 10,000 rpm to obtain a precipitate. After adding 0.5M acetic acid solution to dissolve the precipitate, use electrodialysis technology to remove acid and inorganic salts. The electrodialysis membrane is a polyethylene heterogeneous ion exchange membran...

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Abstract

The invention discloses a large-scale preparation method of marine-derived type I collagen subunits. The method comprises the following steps: using marine organism processing byproducts as raw materials, preparing type I collagen by using acetic acid extraction and a salting-out electrodialysis purification integrated technique; using the type I collagen as a raw material, and utilizing a collagenase enzymolysis and multistage membrane separation linkage technology to prepare a type I collagen subunit alpha1 and an alpha2 mixed solution; and on the basis, preparing the type I collagen subunits alpha1 and alpha2 by utilizing a salting-out synergistic electrodialysis technology. Aiming at the technical bottlenecks that only milligram-grade and microgram-grade samples can be prepared and large-scale production cannot be realized in an existing collagen subunit preparation process, a technical system integrating a collagenase enzymolysis synergistic multistage membrane separation linkagetechnology and a salting-out synergistic electrodialysis technology is constructed, and the type I collagen subunits alpha1 and alpha2 are prepared on a large scale.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a large-scale preparation method of marine source type I collagen subunits. Background technique [0002] Collagen is the most abundant protein in vertebrates, and it is the most important structural protein in animal skin and bone connective tissue, accounting for about 30% of the total protein. There are currently 29 types of collagen identified, including collagen types I-XXIX, and each type of collagen has a unique amino acid sequence, structure, and function. Among all collagen types, type I collagen has good biocompatibility, biodegradability and weak antigenicity, and can provide a good source of raw materials for industries such as food, medicine, biofunctional materials and tissue engineering. [0003] In recent years, due to the occurrence of infectious diseases such as avian influenza, mad cow disease, and foot-and-mouth disease, the global d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K14/78C07K1/36C07K1/34C07K1/30
CPCC12P21/06C07K14/78Y02A20/124
Inventor 陈俊德高然
Owner THIRD INST OF OCEANOGRAPHY MINIST OF NATURAL RESOURCES