Preparation method and application of Chinese breast milk triglyceride substitute fat
A technology of triglyceride and palmitic acid triglyceride, applied in the field of synthetic human milk substitutes, can solve problems such as large differences, and achieve the effects of abundant sources, increased yield and high nutritional value
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[0086] A kind of preparation method of Chinese breast milk triglyceride substitute fat of the present invention comprises the following steps:
[0087] S1. Palm stearin is catalyzed by sodium methoxide, neutralized by citric acid, washed with warm water, bleached with clay and washed with ethanol to obtain 52° palm stearin;
[0088] S2. get sunflower oil, high oleic sunflower oil and perilla seed oil by proportioning to prepare free fatty acid, make mixed oil after mixing with lauric acid, myristic acid and step S1 gained 52 ° of palm stearin, mix to Add directional lipase enzymatic hydrolysis catalysis to oil to obtain Chinese breast milk triglyceride substitute fat;
[0089] S3. Detect and evaluate the Chinese breast milk triglyceride substitute fat obtained in step S2, and obtain the similarity score MD between the sn-2 fatty acid composition and triglyceride configuration in the substitute fat and Chinese breast milk.
[0090] The process of adding directional lipase enzy...
Embodiment 1
[0112]Weigh 10gsn-2 palmitic acid triglyceride in a reaction bottle, and add free fatty acids, lauric acid, and myristic acid from sunflower oil, high oleic sunflower oil, perilla seed oil, and mix with 1:6 molar ratio, 8 wt% of Novozyme 435 lipase (based on the total mass of the substrate) was added thereto, the reaction flask was filled with nitrogen and sealed, and reacted in a water bath at 60° C. at a stirring rate of 200 rpm for 8 h, and repeated three times. The triglyceride fatty acid composition, distribution and triglyceride configuration of the obtained product, as well as the model comparison score with Chinese breast milk, the specific results are shown in Table 4 below.
[0113] The obtained product is mixed with 65% of desalted whey powder, 20% of maltodextrin, 0.5% of vitamins and 0.5% of minerals, and spray-dried to prepare a dietary food for infants and young children.
[0114] Table 4 Fatty acid composition and distribution and triglyceride configuration of ...
Embodiment 2
[0118] Weigh 400g sn-2 palmitic acid triglyceride in a reaction flask, and add free fatty acids, lauric acid, and myristic acid from sunflower oil, high oleic sunflower oil, perilla seed oil, and mix in a molar ratio of 1:6 , 8 wt% of Lipozyme RM IM lipase (based on the total mass of the substrate) was added thereto, the reaction flask was filled with nitrogen and sealed, and reacted at a stirring rate of 200 rpm for 8 h in a water bath at 60 °C, and repeated three times. The triglyceride fatty acid composition, distribution and triglyceride configuration of the obtained product, as well as the model comparison score with the Chinese breast milk, the specific results are shown in Table 5 below.
[0119] The obtained product is mixed with 65% desalted whey powder, 20% maltodextrin, 0.8% fructo-oligosaccharide, 1% galacto-oligosaccharide, 0.5% vitamin and 0.5% mineral, and spray-dried to prepare a diet for infants and young children Food, used to eliminate infant constipation, i...
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