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Preparation method of selenium nano double-emulsified micro-preparation

A selenium nano- and double-emulsion technology, which is applied in the direction of anti-toxic agents, pharmaceutical formulas, emulsion delivery, etc., can solve the problems of inability to accurately control the absorption and release properties, and achieve the effects of enriching nutrition, inducing apoptosis, and facilitating preservation

Inactive Publication Date: 2021-02-05
CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims at the problems that chitosan-selenium preparations are applied to oral delivery systems, and the absorption and release properties cannot be precisely controlled. The present invention adopts whey protein with a natural cavity structure, compounded with electronegativity food-grade gum arabic, through To simulate the gastrointestinal fluid conditions in vivo, explore the stability and precise release characteristics of the double-emulsion micro-nanocapsule system with the gum arabic-whey protein complex as the wall material and the chitosan-selenium nano-preparation as the aqueous core; Meanwhile, the in vivo and in vitro antitumor properties of the chitosan-selenium system were evaluated

Method used

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  • Preparation method of selenium nano double-emulsified micro-preparation
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  • Preparation method of selenium nano double-emulsified micro-preparation

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preparation example Construction

[0040] A method for preparing a selenium nanometer double-emulsion micropreparation with sustained-release properties, nutritional supplementation and cancer cell inhibition functions, comprising the following steps:

[0041] 1) using chitosan as a template to prepare the W1 phase of the chitosan-selenium nano-preparation;

[0042] 2) Add the chitosan-selenium nano-preparation W1 phase obtained in step (1) into the O phase container containing soybean oil-vitamin E, carry out high-pressure homogenization, and form the colostrum of W1 / O;

[0043] 3) Add the W1 / O colostrum obtained in step (2) to the W2 composite solution of the polysaccharide-whey protein stock solution after adjusting the pH to prepare the W1 / O / W2 double emulsion.

Embodiment 1

[0044] The preparation of embodiment 1 chitosan-selenium (W1 phase) of the present invention

[0045] In situ reduction of H using Vc 2 SeO 3 Dissolve 0.01g~10g chitosan gel in 50mL ultrapure water, stir with a magnetic stirrer at a speed of 100~1000r / min, after stirring for 0~48h, slowly add 1~50mL, 0.1~50mM / L Selenous acid solution, after reacting for 1 hour, add 1-50mL, 1-100mmol / L Vc solution, stir well until the solution turns bright orange-red. Then dilute the orange-red solution to 100-500mL and continue stirring thoroughly for 1-36 hours, then store it in a refrigerator at -20-10°C.

Embodiment 2

[0046] Embodiment 2 Preparation of selenium micro-nano double emulsion preparation of the present invention

[0047] 1. Prepare a whey protein stock solution with a concentration of 1% to 30% (w / w): dissolve the whey protein powder in water, stir it magnetically for 2 hours at room temperature, and then let it stand overnight at 4°C to ensure complete Dissolves hydration. The next day, it was equilibrated to room temperature, and heated in a water bath at 80°C for 30 minutes to fully denature it. A gum arabic solution with a concentration of 0.5% to 5% (w / w) is prepared. The concentrations of whey protein isolate and pectin stock solutions were prepared to be 10% and 2% respectively.

[0048] 2. Preparation of polysaccharide and protein mixed system: mix polysaccharide and whey protein isolate solution according to a certain volume ratio and continuously and gently stir magnetically, wherein the concentration of whey protein isolate in the final mixed system is fixed at the ...

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Abstract

The invention discloses a preparation method of a selenium nano double-emulsified micro-preparation with gastrointestinal tract slow release, nutritional supplement and cancer cell inhibition functions. The preparation method comprises the following steps of 1) preparing a W1 phase of a chitosan-selenium nano-preparation by using chitosan as a template; (2) adding the chitosan-selenium nano preparation W1 phase obtained in the step (1) into an O-phase container containing soybean oil-vitamin E, and carrying out high-pressure homogenization to form a primary emulsion of W1 / O; and (3) adjustingthe pH value of the W1 / O primary emulsion prepared in the step (2), and adding the W1 / O primary emulsion into a W2 composite solution of a polysaccharide-whey protein stock solution to prepare the double emulsion. According to the method, a double-emulsification technology is adopted to prepare a double-emulsification oral delivery system with a core-shell structure. The selenium nano double-emulsified micro-preparation prepared by the method disclosed by the invention can effectively induce HepG2 apoptosis in vitro and inhibit the dosage of tumor growth in vivo.

Description

technical field [0001] The invention relates to a preparation method of a selenium nanometer double-emulsion micropreparation with the functions of sustained release, nutritional supplement and inhibition of cancer cells, and the release control of the micropreparation in gastrointestinal tract fluid and the evaluation of its effect of inhibiting cancer cells. Background technique [0002] Selenium (Selenium) is an essential micronutrient element for the human body, which has anti-oxidation, anti-cancer, anti-bacterial and other effects. Most areas in my country are in a state of selenium deficiency. Due to the narrow range of effective dose and toxic dose of selenium, surface modification of selenium by micro-nano technology is a commonly used method at present. Traditional non-surface-modified nano-selenium particles have poor stability, which affects the controllability of their dosage and release properties. [0003] At present, the stability and oxidation resistance of...

Claims

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Application Information

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IPC IPC(8): A61K9/113A61K33/04A61K47/22A61K47/36A61K47/42A61K47/44A61K47/61A61P31/04A61P33/02A61P35/00A61P39/06
CPCA61K9/1075A61K9/113A61K33/04A61K47/22A61K47/36A61K47/42A61K47/44A61K47/61A61P31/04A61P33/02A61P35/00A61P39/06
Inventor 冷小京宋萧萧陈钰莹孙红波王博览柴玉
Owner CHINA AGRI UNIV
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