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Multifunctional composite microbial agent and preparation method and application thereof

A compound microbial bacterial agent and multi-functional technology, applied in the field of multifunctional compound microbial bacterial agent and its preparation, can solve the problem of single function and achieve the effects of improving utilization rate, inhibiting growth, and promoting digestion

Pending Publication Date: 2021-02-05
中诚国联(河南)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing microbial agents have been applied to aquaculture, but the functions of existing microbial agents are relatively single and have limitations

Method used

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  • Multifunctional composite microbial agent and preparation method and application thereof
  • Multifunctional composite microbial agent and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Inoculate 20 parts of Pediococcus lactis, 20 parts of Bacillus licheniformis, 15 parts of Rhodopseudomonas marsh, 10 parts of Saccharomyces cerevisiae, 10 parts of leech vibrio and 10 parts of Streptococcus faecalis to the respective solid culture Activated culture in medium.

[0027] (2) Then inoculate the activated Pediococcus lactis, Bacillus licheniformis, Rhodopseudomonas marsh, Saccharomyces cerevisiae, Vibrio leech and Streptococcus faecalis into the corresponding liquid medium for expansion culture.

[0028] (3) Then the expanded Pediococcus lactis, Bacillus licheniformis, Rhodopseudomonas marsh, Saccharomyces cerevisiae, Hirudovibrio and Streptococcus lactis were mixed and inoculated into the fermentation medium, and 2×10 4 portion of water, and cultured for 6 days to obtain a microbial inoculum.

[0029] The solid medium in step 1) includes: peptone 1.2g / 100mL, 2.8g / 100mL gelatin, glucose 1.9g / 100mL, dipotassium hydrogen phosphate 0.05g / 100mL, and agar 1....

Embodiment 2

[0036] (1) 25 parts of Pediococcus lactis, 25 parts of Bacillus licheniformis, 20 parts of Rhodopseudomonas marsh, 15 parts of Saccharomyces cerevisiae, 15 parts of leech vibrio and 15 parts of Streptococcus faecalis were inoculated into respective solid culture Activated culture in medium.

[0037] (2) Then inoculate the activated Pediococcus lactis, Bacillus licheniformis, Rhodopseudomonas marsh, Saccharomyces cerevisiae, Vibrio leech and Streptococcus faecalis into the corresponding liquid medium for expansion culture.

[0038] (3) Then the expanded Pediococcus lactis, Bacillus licheniformis, Rhodopseudomonas marsh, Saccharomyces cerevisiae, Hirudovibrio and Streptococcus lactis were mixed and inoculated into the fermentation medium, and 2×10 4 portion of water, and cultured for 6 days to obtain a microbial inoculum.

[0039] The solid medium in step 1) includes: peptone 1.2g / 100mL, 2.8g / 100mL gelatin, glucose 1.9g / 100mL, dipotassium hydrogen phosphate 0.05g / 100mL, and aga...

Embodiment 3

[0046] (1) Inoculate 30 parts of Pediococcus lactis, 30 parts of Bacillus licheniformis, 25 parts of Rhodopseudomonas swamp, 20 parts of Saccharomyces cerevisiae, 20 parts of leech vibrio and 20 parts of Streptococcus faecalis to respective solid culture Activated culture in medium.

[0047] (2) Then inoculate the activated Pediococcus lactis, Bacillus licheniformis, Rhodopseudomonas marsh, Saccharomyces cerevisiae, Vibrio leech and Streptococcus faecalis into the corresponding liquid medium for expansion culture.

[0048] (3) Then the expanded Pediococcus lactis, Bacillus licheniformis, Rhodopseudomonas marsh, Saccharomyces cerevisiae, Hirudovibrio and Streptococcus lactis were mixed and inoculated into the fermentation medium, and 2×10 4 portion of water, and cultured for 6 days to obtain a microbial inoculum.

[0049] The solid medium in step 1) includes: peptone 1.2g / 100mL, 2.8g / 100mL gelatin, glucose 1.9g / 100mL, dipotassium hydrogen phosphate 0.05g / 100mL, and agar 1.5g / 1...

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Abstract

The invention is applicable to the technical field of microbial agents, and provides a multifunctional composite microbial agent which comprises the following components in parts by weight: 20-40 parts of pediococcus acidilactici, 20-40 parts of bacillus licheniformis, 15-35 parts of rhodopseudomonas palustris, 10-30 parts of saccharomyces cerevisiae, 10-30 parts of bdellovibrio bacteriovorus and10-30 parts of lactic streptococcus faecalis. Through combined use of the pediococcus acidilactici, the bacillus licheniformis and the rhodopseudomonas palustris, the multifunctional composite microbial agent can increase the utilization rate of feed remarkably, promote digestion of aquatic animals and inhibit growth of harmful bacteria effectively so as to promote growth of the aquatic animals and prevent outbreak of diseases; and the bdellovibrio bacteriovorus has an obvious removal effect on escherichia coli and other pathogenic bacteria in water, and has a removal effect on ammonia nitrogen, nitrite and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial inoculants, and in particular relates to a multifunctional composite microbial inoculum and its preparation method and application, as well as its preparation method and application. Background technique [0002] Aquatic products are the general term for aquatic animal and plant products and their processed products produced by marine and freshwater fisheries. Including: fish, shrimp, crab, shellfish, algae, sea animals and other fresh products produced by fishing and aquaculture; processed products that have been frozen, pickled, dried, smoked, cooked, canned and comprehensively utilized. Aquatic food is rich in nutrients and has different flavors. Fish meal and concentrated fish juice made from low-value fish and processed waste are important protein feeds. Protein aquatic products made from aquatic animals and plants, such as oils, glues, vitamins, hormones and other products, are chemical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A23K10/18A01N63/20A01P1/00C02F3/34C02F101/16C02F103/20C12R1/01C12R1/10C12R1/865C12R1/46
CPCC12N1/20C12N1/16A23K10/18A01N63/20C02F3/34C02F2101/16C02F2101/166C02F2103/20A23V2400/225A23V2400/413
Inventor 祝春华朱永杰石朋飞陶俊杰
Owner 中诚国联(河南)生物科技有限公司
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