Application method of chitosan/glucan-containing moss sporophyte suspension to bare land greening

An application method and suspension technology, applied in the directions of glucan adhesive, application, horticultural method, etc., can solve the problems of failure to cover green, poor adhesion of moss, irritating odor, etc., and achieve high coverage and stickiness. The effect of good bonding effect, improving adhesion and air permeability

Active Publication Date: 2021-02-09
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the development of moss greening projects, the cultivation and processing technology of moss materials is particularly important, especially in the cultivation of moss sporophytes, because the moss sporophytes will fail to cover green due to lack of nutrition when they are bon

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] A method for applying a moss sporophyte suspension containing chitosan / dextran to cover green on bare ground, comprising the following steps:

[0029] Step 1. Collect the spore capsules of Sagittarius moss. When the spore caps of the spore capsules begin to fall off, cut the spore capsules, collect the spores, and wash out the remaining spores of the spore capsules with distilled water; add 3% w / w peroxide to the spores Hydrogen solution, after shaking for 6 minutes, obtain a mixed solution of sterilized spores and hydrogen peroxide; the volume ratio of the spores to 3% w / w hydrogen peroxide solution is 1:10;

[0030] Step 2, inoculate the mixed solution of 2 mL of sterilized spores and hydrogen peroxide into 10 mL of spore germination induction medium, carry out spore germination induction culture for 30 days, and obtain a suspension containing spores and protonema; the spore germination induction culture conditions are: Temperature 22 ℃, light intensity 2500lx, the li...

Embodiment 2

[0038] A method for applying a moss sporophyte suspension containing chitosan / dextran to cover green on bare ground, comprising the following steps:

[0039] Step 1. Collect the spore capsules of Sagittarius moss. When the spore caps of the spore capsules begin to fall off, cut the spore capsules, collect the spores, and wash out the remaining spores of the spore capsules with distilled water; add 4% w / w peroxide to the spores Hydrogen solution, after shaking for 10 minutes, a mixture of sterilized spores and hydrogen peroxide was obtained; the volume ratio of the spores to 4% w / w hydrogen peroxide solution was 1:10;

[0040] Step 2, inoculate the mixed solution of 2mL sterilized spores and hydrogen peroxide into 10mL spore germination induction medium, carry out spore germination induction culture for 50 days, obtain the suspension containing spores and protonema; the spore germination induction culture conditions are: The temperature is 28°C, the light intensity is 3000lx, t...

Embodiment 3

[0048] A method for applying a moss sporophyte suspension containing chitosan / dextran to cover green on bare ground, comprising the following steps:

[0049] Step 1. Collect the spore capsules of Sagittarius moss. When the spore caps of the spore capsules begin to fall off, cut the spore capsules, collect the spores, and wash out the remaining spores of the spore capsules with distilled water; add 3.5% w / w peroxide to the spores Hydrogen solution, after shaking for 8 minutes, obtain a mixed solution of sterilized spores and hydrogen peroxide; the volume ratio of the spores to 3.5% w / w hydrogen peroxide solution is 1:10;

[0050] Step 2, inoculate the mixed solution of 2 mL of sterilized spores and hydrogen peroxide into 10 mL of spore germination induction medium, carry out spore germination induction culture for 40 days, and obtain a suspension containing spores and protonema; the spore germination induction culture conditions are: Temperature 25 ℃, light intensity 2800lx, th...

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Abstract

The invention discloses an application method of a chitosan/glucan-containing moss sporophyte suspension to bare land greening. The application method comprises the following steps of collecting mossspores, and adding a hydrogen peroxide solution into the spores to obtain a mixed solution of disinfected spores and hydrogen peroxide; inoculating the mixed solution of the disinfected spores and thehydrogen peroxide into a spore germination induction culture medium for spore germination induction culture, adding into a solid subculture multiplication culture medium for subculture multiplicationculture after culture, mixing with a liquid culture medium after culture, and carrying out shake culture to obtain a dark green suspension; adding chitosan, glucan, ferric ammonium citrate, phenoxyacetic acid, zinc sulfate and potassium nitrate into the dark green suspension to obtain the moss sporophyte suspension; uniformly smearing a moss adhesive on bare land, then uniformly spraying the mosssporophyte suspension on the surface of the moss adhesive, using a sunshade net for covering, and carrying out spray irrigation in time. The moss sporophyte suspension is used for bare land greening,and the greening covering degree is high after growth.

Description

technical field [0001] The invention relates to the technical field of bare ground greening, in particular to a method for applying chitosan / glucan-containing moss sporophyte suspension to bare ground greening. Background technique [0002] At present, bryophytes are low-level higher plants, which are widely distributed in nature. Bryophytes have the habit of clustering and dormancy, have strong water absorption capacity, fast growth speed and strong cold resistance, and are not easy to get sick and cause insect pests. It is a pioneer plant that can rapidly divide and reproduce in a suitable environment. Absorb and accumulate dust particles and other substances in the air to form soil. At the same time, moss can also change the humidity, pH and other indicators of the surrounding environment to form a soil suitable for the survival of other higher plants. Improve the environment where vegetation cannot grow. [0003] With the development of the economy, land greening and b...

Claims

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Application Information

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IPC IPC(8): A01G22/30A01H4/00C09J105/02C09J105/08C09J189/00C09J123/08C09J11/04C09J11/06C09J11/08
CPCA01G22/30A01H4/001A01H4/008A01H4/005C09J105/02C09J11/04C09J11/06C09J11/08C08L2205/035C08L5/08C08L89/00C08L23/0853C08K13/02C08K5/09C08K3/26C08K5/34922C08K5/21C08K7/26C09J105/00C08H1/06C05G5/27C05C3/00C08L89/06C05C5/02C05C9/00C05D5/00C05D9/02C04B7/345C05F11/00
Inventor 孙庚类延宝陈珂夏红霞朱大林任惠
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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