The invention discloses a preparation method of a bonding agent for a paper tube. The preparation method comprises the following steps of 1, placing deionized water, polyvinyl alcohol and the like in a reaction kettle, performing stirring and temperature rise, adding sodium dodecyl benzene sulfonate and the like, and performing stirring, 2, adding alkylphenol polyvinylether carboxymethyl sodium and the like, and continuing to perform stirring, 3, adding a konjak powder solution and the like, and continuing to perform stirring, 4, adding compound starch and the like, and performing stirring and temperature rise, and 5, adding an enzymatic hydrolysate and the like, and performing stirring and filtering, wherein a preparation method of the konjak powder solution comprises the concrete steps of uniformly mixing konjak powder, cetyl trimethyl ammonium bromide and the like, adding a chloroauric acid solution, performing stirring until a brilliant yellow color becomes clarified, adding a concentrated HCl solution, performing stirring and standing, adding a chitosan solution, performing mixing, and performing ultrasonic treatment and centrifugation to take a supernate. The prepared bonding agent has the benefits that the bonding agent is short in paper fiber breaking time, high in bonding strength, good in flowability and good in storage stability.