Fluorescence-radioactivity combined in-vitro targeted screening method

Abstract

The invention provides a fluorescence-radioactivity combined in-vitro targeted screening method. The method comprises the following steps of: binding a drug to be detected to a solid phase carrier, and adding radionuclide labeled target molecules and fluorescent probe labeled same target molecules; after forming competitive bindings, synchronously detecting fluorescence and radioactive signals,obtaining two groups of data results which can be mutually verified and used for more accurately analyzing the binding capacity of the drug and the target molecules, thereby realizing targeted drug screening. The method provided by the invention can realize simultaneous detection of fluorescence and radioactive binding signal intensity of a plurality of to-be-detected drugs through a single test, andrapidly and efficiently screens out drugs with high specific binding capacity to target molecules. The method can effectively reduce the false positive probability, and has the characteristics of high flux, high accuracy, simple operation and the like. With the method adopted, antibody drugs specifically binding to target molecules can be rapidly screened out, pre-target antibodies can be developed for expanding indications of target radiopharmaceuticals in radioactive targeted therapy.

Description

technical field [0001] The invention belongs to the technical field of in vitro targeted screening by biological markers, and in particular relates to an in vitro targeted screening method using fluorescence-radiation combination. Background technique [0002] At present, the commonly used in vitro targeting activity detection methods for specific binding of targeted drugs (such as monoclonal antibodies or peptides, etc.) are mainly based on signal means such as chemiluminescence or fluorescence, and use antigen-antibody binding to perform qualitative and quantitative analysis of immune reactions to achieve in vitro screening. The purpose of producing drugs with target-targeting activity. [0003] In the research of radioactive targeted drugs, chemiluminescent or fluorescent signals are replaced by radioactive signals produced by radionuclide labels. However, each method has certain advantages and disadvantages. The sensitivities of chemiluminescence and fluorescence method...

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Problems solved by technology

However, each method has certain advantages and disadvantages. The sensitivities of chemiluminescence and fluorescence methods are not consistent with radioactive signals, and the first two methods need to be modified with luminescent groups or fluorescent groups on the drug, and the properties of the modified derivatives may be Inconsistent with radiolabeled ligand-modified targeted drugs, so the results cannot be directly applied to radiopharmaceutical targeting

However, radiolabeled screening technology is limited by the nature of nuclides and resource supply status, and requires high hardware requirements for experiments, making it difficult to carry out high-throughput rapid screening research.

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