53 results about "Molecule formation" patented technology

The invention relates to the technical field of single-molecule devices, in particular to a single-molecule device for detecting small-molecule gas based on supramolecules and a preparation method of the single-molecule device. The monomolecular device comprises a functional molecule and a graphene electrode pair, wherein the functional molecule is connected between the graphene electrode pair through an amido bond. A cucurbituril hydrophobic cavity structure in the functional molecular structural formula can contain and capture target small molecular gas, gas molecules of different volumes are selectively included through multiple weak interaction, a dynamically-adjustable subject-object compound structure is formed, and then switching type sensing detection on the small molecular gas is achieved. The amino terminal of the end part of the main bridging unit in the functional molecule can be connected with the carboxyl terminal of the graphene point electrode in a covalent bond manner, and amide condensation molecular chain connection is formed at the two ends of the graphene point electrode, so that the stability of a monomolecular device is improved. The preparation method provided by the invention is simple in process, mild and controllable in reaction condition and beneficial to large-scale production.

A titanium dioxide (TiO2) composition and methods of use, the composition containing surface-modified doped TiO2 nanoparticles (sm-TiO2 NPs) disposed in a polymer matrix material, wherein each sm-TiO2 NP has an outer surface having a plurality of bifunctional linker molecules attached thereto and a plurality of protein molecules linked to the sm-TiO2 NP via the bifunctional linker molecules; and wherein the polymer matrix comprises a polymer precursor component.

The application discloses an HLA-A*11:01 renal cell carcinoma tumor antigen short peptide derived from a long-chain non-coding RNA SLC12A5-AS1 and application thereof. The application provides a tumor antigen peptide, and an amino acid sequence of the tumor antigen peptide is shown as SEQ ID No. 1. It is found by the application that a complex formed by the short peptide and a corresponding HLA molecule (HLA-A*11:01) can be recognized by T cells, and an immune response is activated. The application provides a potential target for renal cell carcinoma treatment, and provides a new target screening direction for subsequent tumor antigen treatment of renal cell carcinoma.

The application discloses a perovskite material, a perovskite thin film, an optoelectronic device and a preparation method thereof, and the chemical general formula of the perovskite material is A'(A x B 1‑x )Pb n X 3n+1 , wherein A' is at least one of a diamino organic molecule, A is methylamine CH3NH3 + , formamidine CH(NH2)2 + , B is Rb + , X is a halogen ion, x is less than 1, and n is a positive integer. The perovskite material is formed by doping Rb + and a long-chain diamino organic molecule to form an organic-inorganic doped perovskite material, and can be applied to the preparation of a perovskite light-emitting layer in an optoelectronic device such as a light-emitting diode. Based on the introduction of Rb + and the diamino organic molecule in the perovskite material, the working life and stability under a current working state can be effectively prolonged.

The invention discloses a PD-L1 monoclonal antibody delivery system as well as a preparation method and application thereof, and belongs to the technical field of medicines. The preparation method comprises the following steps: coupling amino groups of BPA-PEG9 and a PD-L1 monoclonal antibody through NHS (N-hydroxysuccinimide) esterification to form BPA6-aPDL1; the preparation method comprises the following steps: grafting a glucose derivative maltomimetic acid MA of o-benzene dihydroxyl to a lipid-like amphiphilic molecule PEG (Polyethylene Glycol) (1.5 k-DSPE) through an amido bond to form MA4-PEG (1.5 k-DSPE), namely MA4-PD; then, the BPA6-aPDL1 reacts with the MA4-PD through a boric acid ester bond, and finally, the delivery system MB-aPDL1 is formed. The delivery system not only can successfully deliver the PD-L1 to a tumor site across a blood brain barrier, but also can accurately release the PD-L1 monoclonal antibody, so that the PD-L1 monoclonal antibody is only enriched in a tumor region, the residence time of the antibody is prolonged, the tumor anchoring capability of the antibody is improved, and the occurrence of immune-related adverse events is remarkably reduced.

The present invention relates to molecularly imprinted polymers (MIPs), especially those formed around template molecules with magnetic supports (magnetically supported template molecules). The invention allows magnetically supported template molecules to be recycled and reused in further rounds of MIP production thus significantly increasing MIP yields per unit mass of template molecule. Inventive methodologies for forming the magnetically supported template molecules, in particular their functionalised magnetic precursors, facilitates recycling under very mild conditions which leave the recycled protein template molecules intact, thereby maintaining the quality of MIPs formed therefrom.

The invention provides a supramolecular polymer composition capable of co-assembly to maintain a three dimensional (3-D) macrostructural form after 3-D printing, made of a solvent, a template molecule; and a reactive component. The reactive component can be at least one monomer that is capable of hydrogen bonding with the template molecule to form a 1D supramolecular structure. The template may be an amphiphilic polymer. The monomer has at least two pendant groups capable of covalent crosslinking. The invention also includes a 3-D structure formed by crosslinking a 3-D printed supramolecular polymer composition, which optionally has a mesoporous structure. Also included is a method of manufacturing a 3-D structure by delivering a supramolecular polymer composition onto a surface of a substrate to form the 3-D structure.

The application relates to the field of nanomaterial structures and analysis sensing technology, and discloses a core-shell structure Fe3O4@PDA@ZIF-67 peroxidase, a preparation method and application thereof. The core-shell structure Fe3O4 nanoparticle is used as a core, the surface of the Fe3O4 nanoparticle is modified by dopamine to form a polydopamine layer, then ZIF-67 is modified on the surface of the Fe3O4@PDA as a shell, and finally the prepared Fe3O4@PDA@ZIF-67 is heat-treated, so that a stable structure is obtained. In the Fe3O4@PDA@ZIF-67 composite material, the ZIF-67 as the shell can adsorb a signal molecule TMB to form a confined reaction, reduce the loss of active oxygen in the catalytic process, improve the sensitivity of the peroxidase-like enzyme applied to hydrogen peroxide sensing analysis, and has certain guiding significance for the structure design of the peroxidase-like enzyme material.

The invention provides a tissue adhesive material as well as a preparation method and application thereof. The tissue bonding material is prepared from the following raw materials: a polymer monomer, wherein the polymer monomer comprises an acrylic monomer and an acrylamide monomer; the modified chitosan compound is obtained by modifying a chitosan compound through a polyphenol compound, and the polyphenol compound has two or more phenolic hydroxyl groups; and an initiator, wherein the initiator comprises a photoinitiator and a radical initiator. When the tissue bonding material is in contact with a wet or wet tissue surface, interface liquid existing between the bonding material and the wet tissue surface can be quickly absorbed and removed, and carboxyl, amido and catechol groups of the tissue bonding material can form a large number of molecular bonds with molecules on the wet tissue surface, so that instant bonding on the wet tissue surface is formed; and high adhesiveness can be maintained for a long time.

The present application relates to the technical field of organic compound reagent, specifically relates to a silicon-based rhodamine compound and a preparation method thereof, fluorescent modified nucleotide and kit, wherein the fluorescent modified nucleotide can be applied to the nucleic acid sequencing reaction of sequencing by synthesis. The present application creatively improves -COR 11 The connection structure between the fluorescent compound dye core structure adopts a heteroalkyl structure containing dioxygen -O-(CH2) m -O-(CH2) n -COR 11 As the connection structure, the connecting group -COR The silicon-based rhodamine compound is connected to the fluorescent compound core structure, and the fluorescence stability, fluorescence intensity and incorporation efficiency of the modified nucleotide formed by the silicon-based rhodamine compound as a modified molecule used in the nucleic acid sequencing reaction are improved.

The invention discloses an electrochemiluminescence perovskite quantum dot probe with a stable water phase and a preparation method and application thereof. Aiming at the technical bottleneck that the existing perovskite quantum dots are unstable in a water phase and are difficult to be directly used for liquid phase detection, the invention provides a method for preparing an oleylamine / trifluoropropionic acid (OAm / TFPro) double-ligand protected perovskite quantum dot (PQDs) probe by adopting a water phase co-deposition method. The TFPro ligand and water molecules form a hydrogen bond interface through a fluorine-containing group of the TFPro ligand, and a protective layer is constructed on the surfaces of the PQDs, so that the PQDs can be stably dispersed in a water phase for a long time, and efficient electrochemical luminescence (ECL) activity is maintained. An ECL sensing platform constructed based on the probe realizes high-sensitivity and high-selectivity detection of tea polyphenol in tea leaves, and has the advantages of wide linear range, low detection limit, strong anti-interference capability and the like. The method is simple in process and low in cost, and a novel efficient detection tool is provided for the field of food quality safety.

The embodiment of the invention provides a hierarchical structure-property alignment method for molecule generation and editing, and belongs to the technical field of computational chemistry, and the hierarchical structure-property alignment method specifically comprises the following steps: obtaining SMILES representation and property representation; the SMILES representation is extracted into three levels, the property representation is extracted into two levels, and InfoNCE contrast loss is calculated; based on the potential variables and the property characterization, obtaining fusion characterization through a bidirectional cross attention mechanism; constructing an example set based on a molecular skeleton clustering method, screening difficult example molecules to form a challenge set by using reconstruction loss of an auxiliary variational auto-encoder, and gradually introducing challenge set samples by adopting a progressive sampling strategy; constructing a joint training target, and training the model from end to end; after model training is completed, diversified molecular candidates are generated or molecular property modification is carried out. Through the scheme disclosed by the invention, the accuracy and the stability of multi-property molecule editing are improved.

The invention discloses a preparation method and application of a multistage rocket structure nanometer material in the technical field of biomedical materials. Mesoporous silicon dioxide (MSNs) serves as a core, gemcitabine (GEM) is wrapped in the core, the outer layer of the core is coated with calcium carbonate to form a middle layer, halofuginone (HF) is loaded on the middle layer, and the outermost layer is connected with targeting molecules to form a core-shell structure; the nano material has pH and GSH dual responsiveness, calcium carbonate on the outer layer is disintegrated in a subacid environment with the pH being 6.2-6.5, and mesoporous silica (MSNs) on the inner layer is further disintegrated in a 10mM GSH environment; the synthesis method provided by the invention is simple and easy to operate, and the nanoparticles have the advantages of large specific surface area, uniform particle size, good biocompatibility and the like.

The invention discloses functional bioactive glass as well as a preparation method and application thereof, and relates to the technical field of regenerative medical materials.The functional bioactive glass provided by the invention takes silicon, calcium, phosphorus and sodium as basic components, a porous structure is constructed by introducing nano zinc oxide (ZnO), and hydrophilicity and dispersity are improved by utilizing short amylose surface grafting. The nano zinc oxide is used as a pore-forming template and is selectively dissolved out in the subsequent steps to form sphere-like pores with the pore diameter of 1-30nm and the porosity of 30-70%, so that physical support is provided for cell adhesion and bone tissue ingrowth. The short amylose is grafted on the surface of the glass through a covalent bond of an amino silane coupling agent, and hydroxyl (-OH) on a molecular chain of the short amylose and water molecules form a hydrogen bond, so that the contact angle of the material is obviously reduced, and particle aggregation is effectively inhibited.

To provide a computer program, an information processing device, an information processing method, and a learning model generation method capable of performing calculation of interaction between molecules faster than quantum chemical calculation.SOLUTION: The computer program causes a computer to execute a process of acquiring a feature value of a complex and a molecule contained in the complex, acquiring a molecular formation energy of the complex and the molecule contained in the complex output by a learning model by inputting the acquired feature value to the learning model that outputs the molecular formation energy of the complex and the molecule contained in the complex when the feature value of the complex and the molecule contained in the complex is input, and estimating an interaction energy between the molecules contained in the complex based on the acquired molecular formation energy.SELECTED DRAWING: Figure 1

This invention relates to the application of compound A and its derivatives in the preparation of radiopharmaceuticals. The inventors discovered that compound A forms a chelated ligand compound with a target molecule via a linker, which then interacts with the radionuclide. 9 Zr、 47 Sc、 55 Co、 60 Cu、 61 Cu、 62 Cu、 64 Cu、 67 Cu、 66 Ga、 67 Ga、 68 Ga、 82 Rb、 86 Y、 87 Y、 90 Y、 97 Ru、 105 Rh、 109 Pd, 111 In、 117m Sn、 149 Pm, 52 Mn, 149 Tb, 152 Tb, 161 Tb, 99m Tc, 153 Sm、 177 Lu、 186 Re、 188 Re、 199 Au、 201 Tl、 203 Pb, 210 Pb, 212 Pb, 212 Bi、 213 Bi、 225 Ac、 223 Ra and 227 Th can form stable chelates labeled with radioactive metal nuclides under conditions without heating or low temperature. This chelation reaction is highly efficient, and the purity of the chelated product is greater than 95%. Furthermore, the resulting nuclide chelate derivatives exhibit low toxicity and good metabolic properties in vivo, indicating high clinical pharmaceutical potential.

The application discloses an HLA-A*11:01 renal cell carcinoma tumor antigen short peptide derived from a long-chain non-coding RNA LINC02717. The application provides a tumor antigen peptide, and the amino acid sequence of the tumor antigen peptide is shown as SEQ ID No. 1. The application finds that the complex formed by the short peptide and a corresponding HLA molecule (HLA-A*11:01) can be recognized by T cells, and an immune response is activated. The application provides a potential target for renal cell carcinoma treatment, and provides a new target screening direction for subsequent tumor antigen treatment of renal cell carcinoma.

The invention provides a tissue phase homogenizing agent and a preparation method and application thereof, and belongs to the technical field of biological tissue engineering, the homogenizing agent is composed of hydrophilic matrixes (glucose, polyglycerol, poloxamer F127 and polyvinyl alcohol), functional additives (mannose, N-acetylcysteine, sodium hyaluronate and polyethylene glycol) and ultrapure water, and the pH value is 7.0 + / -0.2. Hydrophilic groups and water molecules form a hydrogen bond network, a continuous hydration layer is constructed on a phase interface, phase domain fusion, refractive index homogenization and molecular ordering are achieved, the problem of biological tissue phase homogenization is solved, tissue light transmittance can be improved, the swelling ratio can be stabilized, and the hydrogel has cell proliferation promoting activity and is suitable for tissue transplantation operation and bioengineering tissue preservation.

The invention discloses a nucleic acid molecule delivery carrier as well as a preparation method and application of a freeze-drying preparation of the nucleic acid molecule delivery carrier. Wherein the delivery vehicle comprises a nucleic acid molecule encoding interleukin 12 and an ionizable lipid compound. According to the invention, the mRNA of the interleukin 12 with an optimized sequence is used, the mRNA is more stable, the protein expression is increased, and the misfolding rate is reduced. Meanwhile, the interleukin 12 is anchored through a transmembrane sequence, so that the possible immune storm risk is reduced. Besides, on the basis of newly synthesized, degradable and novel ionizable lipid molecules, a high-efficiency and low-toxicity freeze-drying preparation delivery system based on lipid is formed, and the freeze-drying preparation has potential application value in the fields of preventive vaccines and therapeutic drugs.

A label for analyzing a biological sample includes a first label part comprising a first nucleic acid backbone, and a second label part comprising a second nucleic acid backbone. The first nucleic acid backbone and the second nucleic acid backbone are configured to hybridise at least partially to each other. The label further includes at least one first labelling moiety and at least one second labelling moiety, and at least one guest molecule configured to form a complex with a host molecule.

A thawing solution has 0.1-50 g of a biomimetic material for controlling ice, 0-1.0 mol L−1 of a water-soluble sugar, and balance of a buffer solution. The biomimetic material for controlling ice is a material for controlling ice having an ice-philic group and a hydrophilic group. The hydrophilic group is a functional group that can form a non-covalent interaction with water molecules, and the ice-philic group is a functional group that can form a non-covalent interaction with ice. Using the thawing solution and the thawing reagent prepared from the biomimetic material for controlling ice, which material has ice-philic and hydrophilic properties, the growth and recrystallization of ice crystals can be effectively controlled, and damage to cells or tissue caused by uncontrolled growth of the ice crystals during recovery can be significantly ameliorated.

The present invention relates to a method of screening for a molecule of interest, comprising: (1) a step of preparing a linked construct by linking a plurality of kinds of target molecules to each other, or linking one or more kinds of target molecules and a candidate molecule-nucleic acid conjugate (linking step); and (2) a step of contacting the linked construct in which the plurality of kinds of target molecules are linked to each other with a candidate molecule-nucleic acid conjugate, or contacting the linked construct in which the one or more kinds of target molecules and the candidate molecule-nucleic acid conjugate are linked with a target molecule that is not used in the linking step (contacting step).

This invention discloses a supramolecularly assembled nanophotosensitive agent, its preparation method, and its applications, belonging to the field of organic conjugated molecular assembly technology. Addressing the current technical challenge of single photosensitizers simultaneously achieving high-brightness NIR-II fluorescence, efficient type I ROS generation, and high photothermal conversion efficiency, this invention provides nanoparticles formed by supramolecular self-assembly of a type I photosensitizer and a multi-arm polyethylene glycol derivative, 8-arm-PEG-OH. The multi-arm structure of 8-arm-PEG-OH forms a tightly ordered nano-assembly with DBD molecules through hydrogen bonding, synergistically enhancing the photophysical properties of DBD. The resulting 8P@DBD nanoparticles exhibit a high fluorescence quantum yield of 2.97% in the near-infrared II (NIR-II) region, significantly improved type I reactive oxygen species (ROS) generation efficiency, and a photothermal conversion efficiency of 68.33%. This invention provides a universal strategy for developing high-performance NIR-II type I phototherapeutic reagents and has broad application prospects in biomedicine and other fields.

The present invention relates to a method for screening a target molecule, the method comprising: (1) a step in which a plurality of types of target molecules are bound to each other, or one or more types of target molecules are bound to a candidate molecule-nucleic acid complex, to prepare a conjugate (binding step); and (2) wherein the candidate molecule-nucleic acid complex is brought into contact with a conjugate formed by binding the plurality of types of target molecules, or a step (contacting step) of bringing the target molecules not used in the binding step into contact with a conjugate formed by binding the one or more types of target molecules to a candidate molecule-nucleic acid complex.

A method for detecting the presence and / or amount of a target nucleic acid molecule in a biological sample and a related kit. The method comprises: (a) contacting the biological sample with: i) Cas12b protein, ii) a gRNA targeting a target sequence of the target nucleic acid molecule, and iii) a single-stranded DNA reporter molecule generating a detectable signal after being cleaved to form a reaction mixture; and (b) detecting the presence and / or level of the detectable signal generated in the reaction mixture.

Described herein are immuno-stimulatory RNA nanostructures (which comprises a single-stranded RNA (ssRNA) molecule, wherein the ssRNA molecule forms at least one paranemic cohesion crossover), as well as compositions and methods of use thereof.

The present invention provides a method for manufacturing a dielectric material for capacitors, a method for manufacturing a capacitor, and a capacitor. [Solution] The method comprises generating a polymer chain from monomers by polymerization and functionalizing several repeating units in the polymer chain with reactive groups. The reactive groups branch off from each polymer chain. Furthermore, the reactive groups are suitable for reacting with linker molecules. The reactive groups are therefore suitable for forming covalent bonds with linker molecules. The method also comprises adding linker molecules to the polymer chain. Each linker molecule has at least two chemical linkage points, each of which is suitable for forming covalent bonds with reactive groups. The method further comprises achieving crosslinking of the polymer chain by the reaction of linker molecules having two reactive groups.

The invention discloses a corilagin molecular imprinting electrochemical sensor as well as a preparation method and application thereof. The sensor comprises a glassy carbon electrode substrate, a boric acid functionalized Mxene conductive affinity composite material layer covering the glassy carbon electrode substrate and a corilagin molecularly imprinted polymer layer located on the surface of the boric acid functionalized Mxene conductive affinity composite material layer. The preparation method comprises the following steps: preparing a boric acid functionalized Mxene composite material and preparing a modification liquid; dispensing the modified liquid on the pretreated glassy carbon electrode to prepare a modified electrode; immobilizing corilagin template molecules through boric acid affinity reaction; carrying out surface molecular imprinting polymerization; and eluting the template molecules to form an imprinting cavity, thereby obtaining the sensor. The sensor disclosed by the invention has excellent conductivity, high selectivity and high sensitivity by constructing an integrated structure of a conductive nano skeleton and a dual recognition layer, and is wide in corilagin detection linear range, low in detection limit and good in stability.