26 results about "Molecule formation" patented technology

A titanium dioxide (TiO2) composition and methods of use, the composition containing surface-modified doped TiO2 nanoparticles (sm-TiO2 NPs) disposed in a polymer matrix material, wherein each sm-TiO2 NP has an outer surface having a plurality of bifunctional linker molecules attached thereto and a plurality of protein molecules linked to the sm-TiO2 NP via the bifunctional linker molecules; and wherein the polymer matrix comprises a polymer precursor component.

The invention provides a supramolecular polymer composition capable of co-assembly to maintain a three dimensional (3-D) macrostructural form after 3-D printing, made of a solvent, a template molecule; and a reactive component. The reactive component can be at least one monomer that is capable of hydrogen bonding with the template molecule to form a 1D supramolecular structure. The template may be an amphiphilic polymer. The monomer has at least two pendant groups capable of covalent crosslinking. The invention also includes a 3-D structure formed by crosslinking a 3-D printed supramolecular polymer composition, which optionally has a mesoporous structure. Also included is a method of manufacturing a 3-D structure by delivering a supramolecular polymer composition onto a surface of a substrate to form the 3-D structure.

The present application relates to the technical field of organic compound reagent, specifically relates to a silicon-based rhodamine compound and a preparation method thereof, fluorescent modified nucleotide and kit, wherein the fluorescent modified nucleotide can be applied to the nucleic acid sequencing reaction of sequencing by synthesis. The present application creatively improves -COR 11 The connection structure between the fluorescent compound dye core structure adopts a heteroalkyl structure containing dioxygen -O-(CH2) m -O-(CH2) n -COR 11 As the connection structure, the connecting group -COR The silicon-based rhodamine compound is connected to the fluorescent compound core structure, and the fluorescence stability, fluorescence intensity and incorporation efficiency of the modified nucleotide formed by the silicon-based rhodamine compound as a modified molecule used in the nucleic acid sequencing reaction are improved.

The invention discloses an electrochemiluminescence perovskite quantum dot probe with a stable water phase and a preparation method and application thereof. Aiming at the technical bottleneck that the existing perovskite quantum dots are unstable in a water phase and are difficult to be directly used for liquid phase detection, the invention provides a method for preparing an oleylamine / trifluoropropionic acid (OAm / TFPro) double-ligand protected perovskite quantum dot (PQDs) probe by adopting a water phase co-deposition method. The TFPro ligand and water molecules form a hydrogen bond interface through a fluorine-containing group of the TFPro ligand, and a protective layer is constructed on the surfaces of the PQDs, so that the PQDs can be stably dispersed in a water phase for a long time, and efficient electrochemical luminescence (ECL) activity is maintained. An ECL sensing platform constructed based on the probe realizes high-sensitivity and high-selectivity detection of tea polyphenol in tea leaves, and has the advantages of wide linear range, low detection limit, strong anti-interference capability and the like. The method is simple in process and low in cost, and a novel efficient detection tool is provided for the field of food quality safety.

The embodiment of the invention provides a hierarchical structure-property alignment method for molecule generation and editing, and belongs to the technical field of computational chemistry, and the hierarchical structure-property alignment method specifically comprises the following steps: obtaining SMILES representation and property representation; the SMILES representation is extracted into three levels, the property representation is extracted into two levels, and InfoNCE contrast loss is calculated; based on the potential variables and the property characterization, obtaining fusion characterization through a bidirectional cross attention mechanism; constructing an example set based on a molecular skeleton clustering method, screening difficult example molecules to form a challenge set by using reconstruction loss of an auxiliary variational auto-encoder, and gradually introducing challenge set samples by adopting a progressive sampling strategy; constructing a joint training target, and training the model from end to end; after model training is completed, diversified molecular candidates are generated or molecular property modification is carried out. Through the scheme disclosed by the invention, the accuracy and the stability of multi-property molecule editing are improved.

The invention discloses a preparation method and application of a multistage rocket structure nanometer material in the technical field of biomedical materials. Mesoporous silicon dioxide (MSNs) serves as a core, gemcitabine (GEM) is wrapped in the core, the outer layer of the core is coated with calcium carbonate to form a middle layer, halofuginone (HF) is loaded on the middle layer, and the outermost layer is connected with targeting molecules to form a core-shell structure; the nano material has pH and GSH dual responsiveness, calcium carbonate on the outer layer is disintegrated in a subacid environment with the pH being 6.2-6.5, and mesoporous silica (MSNs) on the inner layer is further disintegrated in a 10mM GSH environment; the synthesis method provided by the invention is simple and easy to operate, and the nanoparticles have the advantages of large specific surface area, uniform particle size, good biocompatibility and the like.

This invention relates to the application of compound A and its derivatives in the preparation of radiopharmaceuticals. The inventors discovered that compound A forms a chelated ligand compound with a target molecule via a linker, which then interacts with the radionuclide. 9 Zr、 47 Sc、 55 Co、 60 Cu、 61 Cu、 62 Cu、 64 Cu、 67 Cu、 66 Ga、 67 Ga、 68 Ga、 82 Rb、 86 Y、 87 Y、 90 Y、 97 Ru、 105 Rh、 109 Pd, 111 In、 117m Sn、 149 Pm, 52 Mn, 149 Tb, 152 Tb, 161 Tb, 99m Tc, 153 Sm、 177 Lu、 186 Re、 188 Re、 199 Au、 201 Tl、 203 Pb, 210 Pb, 212 Pb, 212 Bi、 213 Bi、 225 Ac、 223 Ra and 227 Th can form stable chelates labeled with radioactive metal nuclides under conditions without heating or low temperature. This chelation reaction is highly efficient, and the purity of the chelated product is greater than 95%. Furthermore, the resulting nuclide chelate derivatives exhibit low toxicity and good metabolic properties in vivo, indicating high clinical pharmaceutical potential.

A thawing solution has 0.1-50 g of a biomimetic material for controlling ice, 0-1.0 mol L−1 of a water-soluble sugar, and balance of a buffer solution. The biomimetic material for controlling ice is a material for controlling ice having an ice-philic group and a hydrophilic group. The hydrophilic group is a functional group that can form a non-covalent interaction with water molecules, and the ice-philic group is a functional group that can form a non-covalent interaction with ice. Using the thawing solution and the thawing reagent prepared from the biomimetic material for controlling ice, which material has ice-philic and hydrophilic properties, the growth and recrystallization of ice crystals can be effectively controlled, and damage to cells or tissue caused by uncontrolled growth of the ice crystals during recovery can be significantly ameliorated.

This invention discloses a supramolecularly assembled nanophotosensitive agent, its preparation method, and its applications, belonging to the field of organic conjugated molecular assembly technology. Addressing the current technical challenge of single photosensitizers simultaneously achieving high-brightness NIR-II fluorescence, efficient type I ROS generation, and high photothermal conversion efficiency, this invention provides nanoparticles formed by supramolecular self-assembly of a type I photosensitizer and a multi-arm polyethylene glycol derivative, 8-arm-PEG-OH. The multi-arm structure of 8-arm-PEG-OH forms a tightly ordered nano-assembly with DBD molecules through hydrogen bonding, synergistically enhancing the photophysical properties of DBD. The resulting 8P@DBD nanoparticles exhibit a high fluorescence quantum yield of 2.97% in the near-infrared II (NIR-II) region, significantly improved type I reactive oxygen species (ROS) generation efficiency, and a photothermal conversion efficiency of 68.33%. This invention provides a universal strategy for developing high-performance NIR-II type I phototherapeutic reagents and has broad application prospects in biomedicine and other fields.

A method for detecting the presence and / or amount of a target nucleic acid molecule in a biological sample and a related kit. The method comprises: (a) contacting the biological sample with: i) Cas12b protein, ii) a gRNA targeting a target sequence of the target nucleic acid molecule, and iii) a single-stranded DNA reporter molecule generating a detectable signal after being cleaved to form a reaction mixture; and (b) detecting the presence and / or level of the detectable signal generated in the reaction mixture.

The present invention provides a method for manufacturing a dielectric material for capacitors, a method for manufacturing a capacitor, and a capacitor. [Solution] The method comprises generating a polymer chain from monomers by polymerization and functionalizing several repeating units in the polymer chain with reactive groups. The reactive groups branch off from each polymer chain. Furthermore, the reactive groups are suitable for reacting with linker molecules. The reactive groups are therefore suitable for forming covalent bonds with linker molecules. The method also comprises adding linker molecules to the polymer chain. Each linker molecule has at least two chemical linkage points, each of which is suitable for forming covalent bonds with reactive groups. The method further comprises achieving crosslinking of the polymer chain by the reaction of linker molecules having two reactive groups.

The invention discloses a corilagin molecular imprinting electrochemical sensor as well as a preparation method and application thereof. The sensor comprises a glassy carbon electrode substrate, a boric acid functionalized Mxene conductive affinity composite material layer covering the glassy carbon electrode substrate and a corilagin molecularly imprinted polymer layer located on the surface of the boric acid functionalized Mxene conductive affinity composite material layer. The preparation method comprises the following steps: preparing a boric acid functionalized Mxene composite material and preparing a modification liquid; dispensing the modified liquid on the pretreated glassy carbon electrode to prepare a modified electrode; immobilizing corilagin template molecules through boric acid affinity reaction; carrying out surface molecular imprinting polymerization; and eluting the template molecules to form an imprinting cavity, thereby obtaining the sensor. The sensor disclosed by the invention has excellent conductivity, high selectivity and high sensitivity by constructing an integrated structure of a conductive nano skeleton and a dual recognition layer, and is wide in corilagin detection linear range, low in detection limit and good in stability.

The application provides a kind of nano antibacterial peptide and its preparation method and application, belong to biotechnology field, amino acid sequence is as shown in SEQ ID No.1.The application selects arginine to provide positive charge, selects phenylalanine and tryptophan to provide hydrophobicity;Phenylalanine is selected to construct intermolecular pi-pi bond to provide hydrophobic force driven polypeptide self-assembly;Center selects tryptophan zipper to stabilize the overall intramolecular structure, terminal amidation forms hydrogen bond with water molecules, arginine is located at 2, 3, 8 and 9 to form cation-pi bond with tryptophan to promote the process of molecular self-assembly;Symmetrical structure is used to reduce the cytotoxicity of peptide molecules.The application of nano antibacterial peptide in the preparation of drug for treating infectious diseases of gram-negative bacteria or / and gram-positive bacteria.The antibacterial peptide of the application has broad-spectrum inhibitory effect on pathogenic bacteria, and has low hemolytic activity, the therapeutic index reaches 50.79, and has strong stability in physiological environment.

The application belongs to the technical field of biological medicine, and relates to a liver-targeting compound, a conjugate and application. The liver-targeting compound has a structure shown in formula I, T is a targeting group, B is a branch structure, L1 is a connecting group, L2 is a connecting part between the targeting group and the branch structure, and X is selected from integers between 2 and 6. The liver-targeting compound and the conjugate have clear structures, multiple galactose, galactosamine or galactosamine derivative molecules are linked into a three-cluster or four-cluster molecule to form a molecular cluster form, the affinity to ASGPR is obviously higher than the affinity of a single sugar, the drug liver-targeting delivery capacity can be improved, the delivery efficiency is high, and a long-acting inhibitory effect can be maintained; and the synthesis route is clear, and the preparation process is simple.

The invention relates to a preparation method of a Ca-based metal coordination polymer derived carbon material and SO2 adsorption separation application of the Ca-based metal coordination polymer derived carbon material. The preparation method comprises the following steps: uniformly mixing and grinding a nitrogen-containing ligand and a metal salt through a solvent-free mechanical grinding method, performing prepolymerization to form a metal coordination polymer, performing high-temperature carbonization, acid etching and purification to obtain a nitrogen-doped hierarchical pore carbon carrier, and performing Ca loading to obtain the Ca-based metal coordination polymer derived carbon material with high specific surface area, rich nitrogen sites and a large number of Ca active sites. A rich micropore-mesopore structure is constructed in situ through acid etching, so that diffusion and mass transfer of SO2 molecules are enhanced; rich basic nitrogen sites and SO2 molecules form a strong adsorption effect through a Lewis acid-base effect; the Ca-based active component is formed by decomposing calcium salt in the roasting process and is highly dispersed on the surface or in pore channels of the carrier, agglomeration of the Ca component can be effectively inhibited due to the existence of mesopores, and the adsorption capacity and the cycling stability of the material to acid gases such as SO2 are improved due to the synergism of the three components.

A method of quantifying a concentration of a chemical entity in a matrix includes combining an aqueous buffer; a reporter reagent including at least one reporter molecule selectively bindable to the chemical entity; an organic, non-polar extraction solvent including an internal standard entity; a wash buffer; and the matrix to thereby prepare a liquid precursor. The method includes solvent extracting a sample from the liquid precursor. The sample includes at least one of the internal standard entity and a plurality of complexes formed from the chemical entity and the at least one reporter molecule. The method also includes scanning the sample with a surface-enhanced Raman spectrometer to produce a dataset including at least one of a plurality of target spectra of the chemical entity and a plurality of reference spectra of the internal standard entity. The method includes processing the dataset to thereby quantify the concentration of the chemical entity.

This invention relates to the field of functional materials technology, and discloses an anti-Kasha organic long-afterglow material, its preparation method, and its applications. The anti-Kasha organic long-afterglow material provided by this invention precisely reconstructs the excited-state energy levels of guest molecules through a sulfur oxidation strategy. Using melamine-formaldehyde resin as a rigid matrix, a pure organic long-afterglow material system with both high quantum yield and anti-Kasha type multiple phosphorescence emission is successfully constructed. Compared with the unoxidized system, the singlet-triple bandgap of the oxidized molecules is significantly reduced from 1.264 eV to 0.944 eV, significantly enhancing the intersystem crossing efficiency. At the optimal doping concentration, a phosphorescence quantum yield of up to 63.1% and a total quantum yield of 88.5% are achieved. Furthermore, the oxidized molecules form a dense multi-triple energy level layout, effectively suppressing the internal conversion from the high-energy triplet state to the lowest triplet state in the rigid matrix. For the first time in this type of system, anti-Kasha type multiple room-temperature phosphorescence emission precisely tunable by the excitation wavelength is achieved.

The present disclosure provides a method of removing a target substance from blood of a patient, the method comprising steps of: providing a complexing agent, especially a supra-molecular compound or core particle, adapted for selectively binding a target molecule or target entity in the blood of the patient in a complex, e.g. a supra-molecular complex; administering the complexing agent into the patient's blood, preferably into an extracorporeal blood flow pathway, for binding with the target molecule or the target entity; conveying the blood having the complexing agent through a treatment zone of an extracorporeal blood flow pathway for a predetermined period of time to bind or incorporate the target molecule or target entity within the blood in a complex, such as a supra-molecular complex; and removing the complex (e.g. supra-molecular complex) from the blood by haemodialysis, which preferably includes one or more of filtration, ultrafiltration, convection, or adsorption. The disclosure thus also provides a system (1) for removing a target substance from blood of a patient, the system (1) comprising: an extracorporeal blood flow pathway (2) for connection to a patient and for guiding or conveying a flow of blood from the patient along the pathway; a treatment zone (5) arranged in the extracorporeal blood flow pathway (2) for mixing a complexing agent (C) with the blood adapted to bind a target molecule (M) in a complex (X), especially a supra-molecular complex or core particle complex, as the blood flows through the treatment zone (5); and a haemodialysis unit (4) for separating the complex (X) from the blood via one or more of filtration, ultra-filtration, convection, and membrane adsorption, with or without magnetic assistance.

The application provides a binary high-energy-efficiency molecular communication method based on an imperfect transmitting end, and steps are as follows: in the first step, the transmitting end collects a mixture of two kinds of molecules from the environment as information molecules, and places the two kinds of molecules in two containers with L and H, the two containers constitute an important part of the transmitting end, and the number and concentration of the molecules in the two containers are the same in the initial state; in the second step, the molecules in the containers are moved by consuming energy, so that the concentration distribution of the molecules in the two containers changes, and an imperfect transmitting end is created; according to the concentration changes caused by moving different types of molecules, the judgment mode of the receiving end is given respectively; in the third step, according to the statistical characteristics of the molecules released by the transmitting end, the specific performance of the transmitting end formed by moving different types of molecules is determined in combination with the judgment mode, and the molecular moving mode that makes the energy efficiency of the communication system higher is obtained according to the performance difference. Through the method, a high-efficiency and reliable molecular communication system is realized.

The present invention relates to a vector for delivery of nucleic acids. Specifically, the invention provides a GalNAc molecule, and the GalNAc molecule has a structure as shown in a formula I, or an optical isomer or a raceme of the GalNAc molecule. According to the present invention, the GalNAc molecule is coupled with the small nucleic acid molecule siRNA through the linker to form the GalNAc-siRNA conjugate, and the GalNAc-siRNA conjugate can achieve the excellent delivery efficiency and the excellent gene silencing efficiency of the small nucleic acid molecule siRNA so as to improve the nucleic acid drug treatment effect.

The application provides a preparation method of an engineered DEAR nucleic acid manipulation system, and the preparation method comprises at least one selected from the following: lengthening a substrate recognition region in a RNA molecule of an original DEAR nucleic acid manipulation system to have a length of 7-14 nucleotides; adding a recruitment sequence to a 3' end of a RNA molecule of the original DEAR nucleic acid manipulation system; respectively arranging a first dimerization motif and a second dimerization motif in a III domain in a first RNA molecule and a second RNA molecule from the original DEAR nucleic acid manipulation system, so that the first RNA molecule and the second RNA molecule form a heterodimer, and the like. The preparation method of the engineered DEAR nucleic acid manipulation system provided by the application further improves the specificity and cleavage activity of the DEAR nucleic acid manipulation system based on an RNA ribozyme.

The invention relates to the technical field of biomedical nano-materials and pharmaceutical preparations, and discloses a bionic nano-enzyme medicine for treating gouty arthritis as well as a preparation method and application of the bionic nano-enzyme medicine. The bionic nano-enzyme drug is a nano-particle with a core-shell structure, and sequentially comprises the following components from the inner layer to the outer layer: (1) a catalytic and antioxidant inner core which is formed by compounding carbon dots and manganese dioxide; (2) a molecularly imprinted polymer layer coated outside the inner core, wherein a molecularly imprinted polymer is formed by taking uric acid as a template molecule; and (3) a platelet cell membrane layer coated outside the molecularly imprinted polymer layer. According to the bionic nano-enzyme drug for treating gouty arthritis as well as the preparation method and application thereof, the drug adopts a multi-stage bionic assembly strategy, carbon dots (CDs) are integrated to enhance oxidation resistance, molecular imprinting (MIP) endows uric acid selectivity, and platelet membrane (PLM) camouflage improves targeting and compatibility, so that the drug can be used for treating gouty arthritis. Therefore, safe and efficient synergistic treatment on gouty arthritis is realized.

This invention provides a method for designing molecular circuits that can maintain a constant concentration of molecules that produce an output value. [Solution] A method for designing a molecular circuit that takes molecule D as input and molecule I as output, wherein a simplified model of the molecular circuit uses molecule Ba, molecule Bb, molecule I, molecule M, and molecule D to form the following three reaction systems: TIFF2026069922000011.tif3665 A method comprising a reaction system represented by , wherein the initial concentrations of molecules Ba, Bb, I, M, and D are [Ba]0, [Bb]0, [I]0, [M]0, and [D]0, respectively, and the parameters of Tb and Ti are set such that all of the following conditions are satisfied: (A1) Tb > 0, (A2) Ti > 0, (A3) [Ba]0 + [Bb]0 = Tb, (A4) [I]0 + [M]0 + [D]0 = Ti, and (A5) Ti / Tb << 1.

The present invention relates to, e.g., a method for screening molecules of interest that form complexes with a plurality of types of target molecules, the method comprising: (1) a step for preparing a linked body in which a first target molecule and a second target molecule among the plurality of types of target molecules are linked via a linking part; (2) a step (contact step) for bringing a candidate molecule or a candidate molecule-nucleic acid conjugate into contact with the linked body to form a complex; and (3) a step (cleavage step) for cleaving the linking part after step (2).