Heavy metal ion rapid detection method based on DNA hydrogel

A technology for heavy metal ions and detection methods, which is applied in the field of rapid detection of heavy metal ions based on DNA hydrogel, can solve the problems of complex instruments and professionals in the center, and is not suitable for real-time testing, and achieves low biological toxicity and short preparation time. , The method is simple and effective

Pending Publication Date: 2022-02-15
上海市农产品质量安全中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, methods for detecting heavy metal ions include inductively coupled plasma mass spectrometry, X-ray fluorescence spectrometry, atomic absorption spectrometry, atomic fluorescence spectrometry, and anodic stripping voltammetry. Although these instruments can quantitatively detect heavy metal ions, most of these methods Requires sophisticated instrumentation and specialized staff at the center
These techniques are only suitable for centralized laboratories, not for real-time testing

Method used

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  • Heavy metal ion rapid detection method based on DNA hydrogel
  • Heavy metal ion rapid detection method based on DNA hydrogel
  • Heavy metal ion rapid detection method based on DNA hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Hydroxyamide-DNA strands including A strands and T strands (300-700 μm, dissolved in ultrapure water) were mixed with 5% acrylamide monomer aqueous solution, respectively. The mixed solution was placed in a vacuum desiccator at room temperature (≈15-35° C.) for 10-30 minutes to remove air, and then 1.4% polyammonium sulfate and tetramethylethylenediamine aqueous solution were added. The reaction tubes were immediately placed in a vacuum desiccator to remove air, and polymerized for 15 minutes to produce polyacrylamide-deoxyribonucleic acid conjugates P-SA and P-ST. After the reaction, the polymer product is purified by ultracentrifugal filters with a molecular mass of 50–100 kDa to remove unpolymerized monomers and other small molecules for further use.

[0027] To generate DNA hydrogels for the detection of heavy metal ions, a solution of P-SA oxygen ribozyme strands (100–500 μm in molar ratio) was mixed with an equal amount of substrate strands in triacetate buffer. ...

Embodiment 2

[0030] Hydroxyamide-DNA strands including A strands and T strands (300-700 μm, dissolved in ultrapure water) were mixed with 10% acrylamide monomer aqueous solution, respectively. The mixed solution was placed in a vacuum desiccator at room temperature (≈15-35° C.) for 10-30 minutes to remove air, and then 1.4% polyammonium sulfate and tetramethylethylenediamine aqueous solution were added. The reaction tubes were immediately placed in a vacuum desiccator to remove air, and polymerized for 15 minutes to produce polyacrylamide-deoxyribonucleic acid conjugates P-SA and P-ST. After the reaction, the polymer product is purified by ultracentrifugal filters with a molecular mass of 50–100 kDa to remove unpolymerized monomers and other small molecules for further use.

[0031] To generate DNA hydrogels for the detection of heavy metal ions, a solution of P-ST deoxyribozyme strands (100–500 μm molar ratio) was mixed with an equal amount of substrate strands in triacetate buffer. In o...

Embodiment 3

[0034] Hydroxyamide-DNA strands including A strands and T strands (300-700 μm, dissolved in ultrapure water) were mixed with 8% acrylamide monomer aqueous solution, respectively. The mixed solution was placed in a vacuum desiccator at room temperature (≈15-35° C.) for 10-30 minutes to remove air, and then 1.4% polyammonium sulfate and tetramethylethylenediamine aqueous solution were added. The reaction tubes were immediately placed in a vacuum desiccator to remove air, and polymerized for 15 minutes to produce polyacrylamide-deoxyribonucleic acid conjugates P-SA and P-ST. After the reaction, the polymer product is purified by ultracentrifugal filters with a molecular mass of 50–100 kDa to remove unpolymerized monomers and other small molecules for further use.

[0035]To generate DNA hydrogels for the detection of heavy metal ions, a solution of GR-5 deoxyribozyme strands (100–500 μm molar ratio) was mixed with an equal amount of substrate strands in triacetate buffer. In ord...

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Abstract

The invention discloses aheavy metal ion rapid detection method based on DNA hydrogel. Deoxyribonuclease based on metal ion dependence has high selectivity, specific binding capacity and good stability. The DNA enzyme is a functional nucleic acid and has a catalytic function of protease and a binding function of an antibody, and consists of a loop region for binding to a specific metal ion and hybridizing to its oligonucleotide substrate. After the deoxyribozyme is combined with the metal cofactor, the deoxyribozyme is activated, and the substrate can be divided into two chains. The fluorescent material combined on the chain is activated, the measured fluorescence signal is increased, the fluorescent material is used for sensitive lead quantification, and the detection limit is 1nM. Meanwhile, the detection method based on the DNA hydrogel also shows high selectivity to different metal ions, which is very important for analysis of complex samples.

Description

technical field [0001] The invention belongs to the technical field of detecting heavy metal pollution, and in particular relates to a rapid detection method for heavy metal ions based on DNA hydrogel. Background technique [0002] Heavy metal pollution is an increasingly challenging issue for water environment, soil and drinking water safety. Heavy metal ions are difficult to be degraded by microorganisms, accumulate and strongly interact with cellular proteins, leading to enzyme inactivation and chronic poisoning. For example, lead is one of the most toxic heavy metal ions, which is easy to accumulate in the environment and difficult to degrade. Trace lead exposure can cause serious harm, and long-term lead ions have adverse effects on humans, especially children. This includes severe effects on cardiovascular, reproductive and other metabolic organs and damage to the central nervous system. The maximum concentration of lead ions in drinking water is stipulated as 10μg / ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402
Inventor 邓波丰东升张维谊朱春燕马颖清宋宇迎周雨璊李珊珊杨秋夏
Owner 上海市农产品质量安全中心
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