Milk immunofluorescence detection test strip and application

Abstract

The invention provides a milk immunofluorescence detection test strip and application, and relates to the technical field of detection test strips. The immunofluorescence detection test strip comprises a sample pad, a conjugate pad, a nitrocellulose membrane and absorbent paper which are sequentially assembled and adhered to a PVC bottom plate; the conjugate pad is coated with a fluorescent latexmicrosphere labeled mixed antibody, the nitrocellulose membrane is sequentially coated with an anti-casein antibody (T1 line), an anti-beta lactoglobulin antibody (T2 line), an anti-alpha whey proteinantibody (T3 line), an anti-lactoferrin / bovine serum albumin antibody (T4 line) and a rabbit anti-mouse IgG antibody (C line), and the T1 line, T2 line, T3 line and T4 line are quality control lines.The method can be used for accurately and quantitatively detecting the contents of casein, anti-beta lactoglobulin, anti-alpha whey protein and anti-lactoferrin / bovine serum albumin in food, and is simple and convenient to operate, high in accuracy and high in sensitivity.

Description

technical field [0001] The invention belongs to the technical field of detection test strips, in particular to a milk immunofluorescence detection test strip and its application. Background technique [0002] Food allergens refer to the antigenic substances present in food that can selectively activate human cells, induce specific antibody responses, and cause allergic reactions. Generally speaking, food allergens are proteins or glycoproteins with a molecular weight between 10,000 and 70,000. Common food allergens include milk, beans, eggs, cereals, tree fruits, shellfish, etc. According to the World Health Organization report, among the factors that threaten human health, food allergy ranks No. 4. In recent years, among domestic and foreign food-related warning notifications, the number of allergen issues ranks second, second only to microbial contamination, and has become a hot spot in the industry that is widely concerned internationally. The issue of food allergens h...

AI Technical Summary

Problems solved by technology

In 2012, the promulgation of GB7718-2011 "General Rules for the Labeling of Prepackaged Foods" officially included the labeling of allergens in food into the scope of labeling management for the first time, but no specific regulations have been given for the labeling of allergens. The allergic reaction triggered by this has not attracted enough attention

At present, the methods for detecting milk mainly include enzyme-linked immunoassay and enzyme immunoblotting, which require a long reaction time and are easily affected by changes in enzyme activity, resulting in inaccurate results.

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