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Methylotrophic bacillus bmf 04 water suspension and its preparation method and application

A methylotrophic and bacillus-based technology, applied in the direction of fungicides, microorganism-based methods, bacteria, etc., can solve the problems of late start of development and research, and achieve the effects of low cost, high active ingredients, and good fluidity

Active Publication Date: 2021-06-04
JIANGSU OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing microbial strains used for the development of microbial pesticides are mainly derived from terrestrial environments, and the development and research of microbial strains from the ocean started relatively late. Marine bacteria grow and reproduce quickly and have rich metabolites, which are important germplasm resources for screening resistant strains

Method used

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  • Methylotrophic bacillus bmf 04 water suspension and its preparation method and application
  • Methylotrophic bacillus bmf 04 water suspension and its preparation method and application
  • Methylotrophic bacillus bmf 04 water suspension and its preparation method and application

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Embodiment 1

[0043] Embodiment 1, methylotrophic bacillus (Bacillus methylotrophics) BMF 04 water suspension preparation experiment:

[0044] 1.1 Materials and methods

[0045] 1.1.1 Test strains

[0046] Methylotrophic Bacillus (Bacillus methylotrophics) BMF 04 was isolated, identified and preserved from Lianyungang sea area by our laboratory.

[0047] 1.1.2 Medium

[0048] Strain activation medium: PDA medium. Seed medium: PD medium. Fermentation medium: bran 1.0%, peanut powder 1.0%, CaCl2 1.0%, pH 7.5.

[0049] 1.1.3 Types of additives

[0050] Dispersant: sodium lignosulfonate, calcium dodecylbenzenesulfonate, sodium lauryl sulfate, calcium lignosulfonate, fatty alcohol polyoxyethylene ether 25, Tween 80; thickener: carboxymethyl Sodium cellulose, xanthan gum, soluble starch, polyvinyl alcohol, polyethylene glycol 6000; UV protector: ascorbic acid, sodium fluorescein, dextrin, humic acid, sodium alginate, urea; preservative: paraben Methyl Paraben, Ethyl Paraben, Sodium Benzoat...

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Abstract

The invention discloses a methylotrophic bacillus (Bacillus methylotrophicus) BMF 04 water suspension, the water suspension is composed of a methylotrophic bacillus BMF 04 fermentation liquid and an auxiliary agent, the type of the auxiliary agent and its The weight percent of the fermented liquid is: calcium lignosulfonate 3.0 percent; xanthan gum 0.20 percent; potassium sorbate 0.40 percent; sodium alginate 0.30 percent; soybean oil 0.40 percent. The invention also discloses its preparation method and application. The present invention is a water suspension concentrate developed by using Bacillus methylotrophicus BMF 04 strain. The water suspension concentrate has high active ingredients, simple preparation and processing technology, low loss, and low cost. The most preferred auxiliary agent is obtained through research. A liquid formulation with high suspension rate, good fluidity and strong stability obtained. It can be used for the treatment or prevention of fruit ring disease and / or anthracnose.

Description

technical field [0001] The present invention relates to a kind of bacterial agent, particularly a kind of Bacillus methylotrophicus (Bacillus methylotrophicus) BMF 04 water suspension; The present invention also relates to the preparation method and application of methylotrophic Bacillus (Bacillus methylotrophicus) BMF 04 water suspension . Background technique [0002] Biological control has the advantages of no pollution to the environment, safety to humans and other organisms, long-lasting control effect, and no residue in the product. It can make up for the shortcomings caused by chemical pesticides. It is particularly important to screen more excellent strains that have strong antibacterial effects and promote crop growth. important. The existing microbial strains used for the development of microbial pesticides are mainly derived from terrestrial environments. The development and research of microbial strains from the ocean started relatively late. Marine bacteria gro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/22A01N25/04A01P3/00C12N1/20C12R1/07
CPCA01N25/04A01N63/22C12N1/20
Inventor 马桂珍暴增海冷超凡万丹丹
Owner JIANGSU OCEAN UNIV
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