Oligonucleotide, method and kit for detecting ETV6-RUNX1 fusion gene in sample

A technology of ETV6-RUNX1 and ETV6-F, applied in the fields of life science and biology, can solve the problems of high cost and inferior specificity, and achieve the effect of simple operation, prediction of prognosis, and prevention of clinical recurrence.
CN112430648APending Publication Date: 2021-03-02FUZHOU ADICON CLINICAL LAB INC
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUZHOU ADICON CLINICAL LAB INC
Publication Date
2021-03-02

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Abstract

The invention relates to an oligonucleotide, method and kit for detecting ETV6-RUNX1 fusion gene in a sample. The kit mainly comprises an upstream primer ETV6-F, a downstream primer RUNX1-R and a probe ETV6-Probe for detecting the ETV6-RUNX1 fusion gene, and an upstream primer ABL-F, a downstream primer ABL-R and a probe ABL-Probe for detecting ABI reference gene. The kit is used for detecting thecondition of the ETV6-RUNX1 fusion gene in-vivo acute B lymphocytic leukemia patients, and can conveniently carry out related auxiliary diagnosis and treatment.
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Description

technical field

[0001] The invention belongs to the field of life science and biotechnology, and is specially designed to provide oligonucleotides, methods and kits for detecting ETV6-RUNX1 fusion genes in samples, and uses probe real-time fluorescent PCR technology to detect ETV6-RUNX1 in human B-ALL. RUNX1 fusion gene was detected. Background technique

[0002] The occurrence of leukemia is a complex and multi-step process. Chromosomal mutations can form fusion genes with tumor characteristics, and can also cause uncontrolled expression of some genes that play an important role in the regulation of cell growth and apoptosis, thereby interfering with cell proliferation and differentiation. The normal regulation pathway of , maturation and apoptosis, ultimately leading to leukemogenesis. Leukemia is the most common malignant tumor disease in childhood, with an incidence rate of 3-5 / 100,000, and it is also a disease that seriously threatens children's health. Acute lymphobl...

Claims

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