Pharmaceutical preparation for treating lung diseases, and preparation method thereof

A technology for lung diseases and pharmaceutical preparations, applied in the direction of drug combinations, pharmaceutical formulas, respiratory diseases, etc., to achieve the effect of controllable preparation process and simple preparation process

Active Publication Date: 2021-03-09
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no case of using polyphenol networks for cell drug delivery in existing studies

Method used

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  • Pharmaceutical preparation for treating lung diseases, and preparation method thereof
  • Pharmaceutical preparation for treating lung diseases, and preparation method thereof
  • Pharmaceutical preparation for treating lung diseases, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059]

[0060]

[0061] Preparation Process:

[0062] Weigh 10 mg of bovine serum albumin, dissolve in 5 mL of deionized water; weigh 1.5 mg of FITC, and dissolve in 4 mL of ethanol. The two were added to a round bottom flask and mixed, and reacted in an ice-water bath at 4°C for 8h. The reaction solution was divided into dialysis bags with a molecular weight cut-off of 3500 Da, and dialyzed in deionized water environment to remove free FITC. The fluid in the dialysis bag was collected to obtain FITC-labeled bovine serum albumin for later use. The isolated and purified red blood cells were diluted to 20% with PBS buffer. Weigh 40 mg of tannic acid and dissolve it in 1 mL of deionized water to prepare a tannic acid mother liquor. Accurately weigh 8.9 mg of lanthanum trichloride heptahydrate, and dissolve it in 1 mL of deionized water to prepare a mother liquor of lanthanum trichloride. Add 100 μL of red blood cells to 400 μL of PBS buffer, and mix by inverting up and...

Embodiment 2

[0065]

[0066]

[0067] Preparation Process:

[0068] Weigh 10 mg of bovine serum albumin, dissolve in 5 mL of deionized water; weigh 1.5 mg of FITC, and dissolve in 4 mL of ethanol. The two were added into a round-bottomed flask and mixed, and reacted for 8 hours at 4°C in an ice-water bath. The reaction solution was divided into dialysis bags with a molecular weight cut-off of 3500 Da, and dialyzed in deionized water environment to remove free FITC. The fluid in the dialysis bag was collected to obtain FITC-labeled bovine serum albumin for later use. The isolated and purified red blood cells were diluted to 20% with PBS. Weigh 40 mg of tannic acid and dissolve it in 1 mL of deionized water to prepare a tannic acid mother liquor. Weigh 3.9 mg of ferric chloride hexahydrate and dissolve it in 1 mL of deionized water to prepare ferric chloride hexahydrate mother liquor. Add 100 μL of red blood cells to 400 μL of PBS and mix by inverting up and down. Add 5 μL of tann...

Embodiment 3

[0071]

[0072]

[0073] Preparation Process:

[0074] Weigh 10 mg of bovine serum albumin, dissolve in 5 mL of deionized water; weigh 1.5 mg of FITC, and dissolve in 4 mL of ethanol. The two were added into a round-bottomed flask and mixed, and reacted for 8 hours at 4°C in an ice-water bath. The reaction solution was divided into dialysis bags with a molecular weight cut-off of 3500 Da, and dialyzed in deionized water environment to remove free FITC. The liquid in the dialysis bag was collected to obtain FITC-bovine serum albumin for later use. Dilute RAW264.7 cells with PBS to 2 × 10 6 ~4×10 6 individual / mL. Weigh 40 mg of tannic acid and dissolve it in 1 mL of deionized water to prepare a tannic acid mother liquor. Accurately weigh 8.9 mg of lanthanum trichloride heptahydrate, and dissolve it in 1 mL of deionized water to prepare a mother liquor of lanthanum trichloride. Add 100 μL of RAW264.7 cells to 400 μL of PBS, and mix by inverting up and down. Add 5 μL ...

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Abstract

The invention belongs to the field of drug delivery and the like, and particularly relates to a pharmaceutical preparation for treating lung diseases and a preparation method thereof. The pharmaceutical preparation is characterized by comprising cells, a metal-polyphenol network and drugs wrapped between the cells and the metal-polyphenol network; or comprising cells, polyphenols and drugs wrappedbetween the cells and the polyphenols. The preparation prepared by the invention meets the requirements of a cell preparation; the cell activity, the form and the surface structure are kept unchanged; meanwhile, the targeting and fixed-point release functions are also realized; and meanwhile, a lung long-acting drug delivery system in the invention has the advantages of simple preparation process, controllable preparation process, capability of quickly delivering to the lung and the like.

Description

technical field [0001] The invention belongs to the fields of pharmaceutical preparations, drug delivery and the like, and in particular relates to a pharmaceutical preparation for treating lung diseases and a preparation method thereof. Background technique [0002] According to the World Health Organization (WHO), chronic obstructive pulmonary disease (COPD), lower respiratory tract infections and lung cancer are the third, fourth and sixth causes of death globally, respectively, reflecting the limitations of available treatments. In addition, effective treatments are urgently needed for many other respiratory diseases. Short half-life, poor targeting, and simple traditional dosage forms can no longer meet the needs of many diseases. By designing the drug delivery system, the effectiveness of the drug can be improved while reducing side effects, so that the drug can accumulate in the lesion, increase the targeting of the system, and endow the system with long-term drug re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/06A61K35/18A61K33/244A61K38/38A61K33/26A61K31/7024A61P11/00A61P9/12A61P31/00A61P35/00
CPCA61K45/06A61K35/18A61K33/244A61K38/38A61K33/26A61K31/7024A61P11/00A61P9/12A61P31/00A61P35/00A61K2300/00Y02A50/30
Inventor 何伟肖青青李晓彤
Owner CHINA PHARM UNIV
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