Collagen microfiber hemostatic material and preparation method and application method thereof

Abstract

The invention relates to the field of biological materials, and particularly relates to a collagen microfiber hemostatic material and a preparation method and an application method thereof. The preparation method comprises the following steps of: performing high-speed shearing on a solid collagen material to obtain collagen microfibers, dispersing the collagen microfibers in a water phase, modifying procoagulant active ingredients on the surfaces of the collagen microfibers through connecting molecules, performing freeze-drying treatment, and performing high-speed shearing treatment again to obtain the collagen microfiber hemostatic material. The collagen microfiber hemostatic material can be prepared simply, conveniently and efficiently through high-speed shearing, and the biological activity of the collagen material is kept as much as possible; the length range of the microfibers is 10-1000 microns, and the microfibers have high dispersibility and are favorable for spraying treatment; and the connecting molecules can efficiently and quantitatively load the procoagulant active ingredients on the surfaces of the collagen microfibers, so that the hemostatic effect of the collagen microfibers is enhanced. The collagen microfiber hemostatic material prepared by the preparation method is used for hemostasis of a bleeding wound surface in a spraying manner, such as hemostasis in a brain surgery or liver surgery process, and the effect of non-contact fixed-point hemostasis can be achieved.

Description

technical field [0001] The invention relates to a preparation method of a hemostatic material and a use method thereof, in particular to a collagen microfiber hemostatic material, a preparation method and a use method thereof. Background technique [0002] As a commonly used local hemostatic material in clinical practice, solid collagen material has good hemostatic performance. By combining with platelets, it promotes platelet aggregation, directly activates the endogenous coagulation pathway, and produces coagulation. At the same time, blood cells adhere to form thrombus clots, effectively preventing blood Flush the wound. In addition, collagen can promote the formation of granulation tissue and accelerate wound healing. A large number of studies have shown that the hemostatic activity of collagen materials is significantly better than that of other polysaccharide hemostatic materials, and it can also have a strong affinity with cells and growth factors in the wound healin...

AI Technical Summary

Problems solved by technology

However, due to the large size of the collagen fibers in the raw material collagen silk thread, up to centimeters, the collagen fibers will eventually aggregate into agglomerates without dispersibility, and cannot be used for hemostasis on bleeding wounds by spraying

[0005] For the collagen hemostatic powder material, "CN105597144A" relates to a collagen hemostatic powder, the collagen solution is spray-dried to prepare collagen particles with a particle size range of 0.1-10mm, but the spray-drying temperature is 100-130°C, this temperature The collagen material under treatment has been denatured, which has a great impact on the biological activity and hemostatic effect of collagen

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